SIX2 Polyklonaler Antikörper

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• KD/KO Validated

SIX2 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (6)

Anwendung

WB, IP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

251

Rezensionen (1)

Kat-Nr. : 11562-1-AP

Synonyme

Homeobox protein SIX2, Sine oculis homeobox homolog 2, SIX homeobox 2, SIX2

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Galerie der Validierungsdaten

• 

  WB analysis using 11562-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11562-1-AP (SIX2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 11562-1-AP

  IP result of anti-SIX2 (IP:11562-1-AP, 4ug; Detection:11562-1-AP 1:1000) with HEK-293 cells lysate 1320 ug.

• 

  IF Staining of mouse embryonic kidney using 11562-1-AP

  IF result of SIX2 antibody (11562-1-AP, 1:200) with mouse embryonic kidney rudiment dissected at E13.5 and cultured for 2 days by Dr. Aleksandra Rak-Raszewska. SIX2 positive cells (red) incondense metanephric mesenchyme surrounding the ureteric bud tip.

• 

  IF Staining of heritable frontonasal dysplasia and renal hypoplas using 11562-1-AP

  IF result from Fogelgren B, PMID:18570229, "Misexpression of Six2 is associated with heritable frontonasal dysplasia and renal hypoplasia in 3H1 Br mice.".

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, L02-Zellen, PC-3-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IF: mouse embryonic kidney, heritable frontonasal dysplasia and renal hypoplasia in 3H1 Br mice

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 30 publications below
• IP: See 3 publications below
• ChIP: See 5 publications below

Produktinformation

11562-1-AP bindet in WB, IP, ChIP, ELISA SIX2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Hausschwein, Hund, Kaninchen, Maus, Ratte, Alligator
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SIX2 fusion protein Ag2124
• Vollständiger Name: SIX homeobox 2
• Berechnetes Molekulargewicht: 291 aa, 32 kDa
• Beobachtetes Molekulargewicht: 32-37 kDa
• GenBank-Zugangsnummer: BC024033
• Gene symbol: SIX2
• Gene ID (NCBI): 10736
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The SIX proteins (sine oculis) are a family of homeodomain transcription factors that share a conserved DNA binding domain. Six members (Six1-Six6) of the Six gene family have been identified in mice and humans. SIX2, containing one homeobox DNA-binding domain, is highly expressed in fetal tissues but expression is limited in adult tissues. SIX2 may be involved in limb tendon and ligament development[PMID:21420949]. It has been previously shown that SIX2 is expressed in developing mesenchymal tissue including head and urogenital system at the time of overt midfacial and renal differentiation[PMID: 22282599]. This antibody is a rabbit polyclonal antibody raised against full length SIX2 of human origin.

Publikationen

Species	Application	Title
human		Nature; Kidney organoids from human iPS cells contain multiple lineages and model human nephrogenesis.; Authors - Minoru Takasato
human		Nat Biotechnol; Tubuloids derived from human adult kidney and urine for personalized disease modeling.; Authors - Frans Schutgens
human		Nat Biotechnol; Revealing the spatio-phenotypic patterning of cells in healthy and tumor tissues with mLSR-3D and STAPL-3D.; Authors - Ravian L van Ineveld
mouse		Cell; In Vivo Target Gene Activation via CRISPR/Cas9-Mediated Trans-epigenetic Modulation.; Authors - Hsin-Kai Liao
human		Cell; Tridimensional Visualization and Analysis of Early Human Development.; Authors - Morgane Belle
mouse		Cell Stem Cell; Redefining the in vivo origin of metanephric nephron progenitors enables generation of complex kidney structures from pluripotent stem cells.; Authors - Atsuhiro Taguchi

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Nicolas (Verified Customer) (09-17-2020)

I performed an antigen retrieval (boil in citrate buffer 10mM, pH6 during 12 minutes)

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/200
• Cell Tissue Type: E18.5 kidney

SIX2 Antibody Immunofluorescence validation (1/200 dilution) in E18.5 kidney (Cat no:11562-1-AP)

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