REDD1 specific Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

REDD1 specific Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (5)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

264

Rezensionen (4)

Kat-Nr. : 10638-1-AP

Synonyme

DDIT4, REDD1, RTP801, REDD-1, HIF-1 responsive protein RTP801

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Galerie der Validierungsdaten

• 

  WB analysis using 10638-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10638-1-AP (REDD1 specific antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 cells using 10638-1-AP

  WB result of REDD1 antibody (10638-1-AP, 1:1000) with si-control and si-REDD1 transfected PC-3 cells.

• 

  WB analysis using 10638-1-AP

  Non-treated Hela and Cobalt Chloride treated Hela cells were subjected to SDS PAGE followed by western blot with 10638-1-AP (REDD1 specific antibody) at dilution of 1:600 incubated at room temperature for 6 hours. .

• 

  WB analysis using 10638-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10638-1-AP (REDD1 specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10638-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10638-1-AP (REDD1 specific antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of DU 145 using 10638-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 10638-1-AP (REDD1 specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 10638-1-AP

  IP result of anti-REDD1 specific (IP:10638-1-AP, 3ug; Detection:10638-1-AP 1:500) with MCF-7 cells lysate 2500ug.

• 

  IHC staining of human lung cancer using 10638-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10638-1-AP (REDD1 specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 10638-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10638-1-AP (REDD1 specific antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 10638-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10638-1-AP (REDD1 specific antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 10638-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 10638-1-AP (REDD1 specific antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 10638-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 10638-1-AP (REDD1 specific antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver using 10638-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 10638-1-AP (REDD1 specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 10638-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 10638-1-AP (REDD1 specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of LNCaP using 10638-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed LNCaP cells using REDD1 specific antibody (10638-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, A549-Zellen, DU 145-Zellen, K-562-Zellen, LNCaP-Zellen, mit Cobaltchlorid behandelte HeLa-Zellen, PC-3-Zellen, SH-SY5Y-Zellen
• Erfolgreiche IP: MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Herzgewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: LNCaP-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 38 publications below
• WB: See 246 publications below
• IHC: See 24 publications below
• IF: See 16 publications below
• IP: See 1 publications below
• FC: See 1 publications below
• CoIP: See 2 publications below
• ChIP: See 2 publications below

Produktinformation

10638-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA REDD1 specific und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Eichh?rnchen, Hausschwein, Kaninchen, Maus, Ratte, Mongolische Wüstenrennmaus (Gerbil)
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: REDD1 specific fusion protein Ag0965
• Vollständiger Name: DNA-damage-inducible transcript 4
• Berechnetes Molekulargewicht: 25 kDa
• Beobachtetes Molekulargewicht: 32-35 kDa
• GenBank-Zugangsnummer: BC007714
• Gene symbol: REDD1/DDIT4
• Gene ID (NCBI): 54541
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

REDD1, also named as RTP801 and DDIT4, belongs to the DDIT4 family. REDD1 promotes neuronal cell death. It is a novel transcriptional target of p53 implicated ROS in the p53-dependent DNA damage response. REDD1 controlled cell growth under energy stress, as an essential regulator of TOR activity through the TSC1/2 complex. REDD-1 expression has also been linked to apoptosis, Aβ toxicity and the pathogenesis of ischemic diseases. As an HIF-1-responsive gene, REDD-1 exhibits strong hypoxia-dependent upregulation in ischemic cells of neuronal origin[PMID: 19996311]. In response to stress due to DNA damage and glucocorticoid treatment, REDD-1 is upregulated at the transcriptional level[PMID: 21733849]. REDD-1 negatively regulates the mammalian target of Rapamycin, a serine/threonine kinase often referred to as mTOR[PMID: 22951983]. It is crucial in the coupling of extra- and intracellular cues to mTOR regulation. The absence of REDD-1 is associated with the development of retinopathy, a major cause of blindness[PMID: 22304497]. REDD1 is a new host defense factor, and chemical activation of REDD1 expression represents a potent antiviral intervention strategy[PMID: 21909097]. The calculated molecular weight of REDD1 is 25 kDa. Because of multiple lysines in the proteins, REDD1 offen migrates around 35 kDa on Western blot[PMID: 19221489]. This antibody is a rabbit polyclonal antibody raised against full length human REDD1 antigen. This antibody is specific to the REDD1 from siRNA experiment (PMID:24713927)

Publikationen

Species	Application	Title
mouse	WB	Cell; Plzf regulates germline progenitor self-renewal by opposing mTORC1.; Authors - Hobbs Robin M RM
human	WB	Cell; Acid Suspends the Circadian Clock in Hypoxia through Inhibition of mTOR.; Authors - Zandra E Walton
mouse	WB	Cancer Cell; A UBE2O-AMPKα2 Axis that Promotes Tumor Initiation and Progression Offers Opportunities for Therapy.; Authors - Isabelle K Vila
rat	WB	Nat Med; REDD1 is essential for stress-induced synaptic loss and depressive behavior.; Authors - Kristie T Ota
mouse	chIP	Nat Immunol; The transcription factor Rfx7 limits metabolism of NK cells and promotes their maintenance and immunity.; Authors - Wilson Castro
mouse	WB	Acta Neuropathol; MEF2 impairment underlies skeletal muscle atrophy in polyglutamine disease.; Authors - Samir R Nath

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Yuki (Verified Customer) (11-10-2022)

This product was used for IHC in human tissue.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: kidney

REDD1 specific Antibody Immunohistochemistry validation (1:50 dilution) in kidney (Cat no:10638-1-AP)

FH

Stephane (Verified Customer) (11-26-2020)

The antibody performs great on human cell extract. It detects specific bands with the REDD1 band resolving around 30-35 kD.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: 293T

REDD1 specific Antibody Western Blot validation (1:1000 dilution) in 293T (Cat no:10638-1-AP)

FH

Jamal (Verified Customer) (09-17-2019)

This product was used for Western Blotting in mouse cortex tissue.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse Brain Tissue

FH

Bradley (Verified Customer) (08-19-2019)

I have used this antibody on numerous occasions with success. The antibody performs great on human cell extract, as evidenced by a single immunoreactive band. The antibody performs moderately well on mouse skeletal muscle extract as it detects many non-specific bands with the REDD1 band resolving around 32-35 kD (specificity determined using REDD1-/- samples). The antibody is capable of detecting large changes in REDD1 protein in mouse skeletal muscle (i.e. induction following dexamethasone treatment), but smaller changes are difficult to assess in this tissue. This antibody will detect smaller changes in human cell lines such as HEK 293 or Hela cells.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500 for mouse skeletal muscle. 1:1000 for human cells
• Cell Tissue Type: Mouse skeletal muscle. HEK293 cells

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