PFKFB3 Polyklonaler Antikörper

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• KD/KO Validated

PFKFB3 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

110

Rezensionen (5)

Kat-Nr. : 13763-1-AP

Synonyme

IPFK2, Fructose-2,6-bisphosphatase, 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3, 6-phosphofructo-2-kinase, 6PF-2-K/Fru-2,6-P2ase brain/placenta-type isozyme

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Galerie der Validierungsdaten

• 

  WB analysis using 13763-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13763-1-AP (PFKFB3-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 13763-1-AP

  WB result of PFKFB3-Specific antibody (13763-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PFKFB3-Specific transfected HEK-293 cells.

• 

  WB analysis of mouse thymus using 13763-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 13763-1-AP (PFKFB3-Specific antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 13763-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 13763-1-AP (PFKFB3-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 13763-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 13763-1-AP (PFKFB3-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse spleen using 13763-1-AP

  IP result of anti-PFKFB3 (IP:13763-1-AP, 4ug; Detection:13763-1-AP 1:500) with mouse spleen tissue lysate 2440 ug.

• 

  IP experiment of mouse spleen using 13763-1-AP

  IP result of anti-PFKFB3 (IP:13763-1-AP, 4ug; Detection:13763-1-AP 1:300) with mouse spleen tissue lysate 3920 ug.

• 

  IP experiment of mouse spleen using 13763-1-AP

  IP result of anti-PFKFB3 (IP:13763-1-AP, 4ug; Detection:13763-1-AP 1:300) with mouse spleen tissue lysate 3920 ug.

• 

  IHC staining of human liver cancer using 13763-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13763-1-AP (PFKFB3-Specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 13763-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13763-1-AP (PFKFB3-Specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 13763-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 13763-1-AP (PFKFB3-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 13763-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 13763-1-AP (PFKFB3-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 13763-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 13763-1-AP (PFKFB3-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 13763-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 13763-1-AP (PFKFB3-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 13763-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PFKFB3-Specific antibody (13763-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, A431-Zellen, HeLa-Zellen, Jurkat-Zellen, Mausherzgewebe, Mausmilzgewebe, Maus-Thymusgewebe
• Erfolgreiche IP: Mausmilzgewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Lebergewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 19 publications below
• WB: See 98 publications below
• IHC: See 23 publications below
• IF: See 16 publications below
• IP: See 4 publications below
• FC: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

13763-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA PFKFB3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PFKFB3 fusion protein Ag4744
• Vollständiger Name: 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3
• Berechnetes Molekulargewicht: 520 aa, 60 kDa
• Beobachtetes Molekulargewicht: 58 kDa
• GenBank-Zugangsnummer: BC040482
• Gene symbol: PFKFB3
• Gene ID (NCBI): 5209
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PFKFB3, also named as NY-REN-56 and iPFK-2, plays a role in glucose metabolism. Its synthesis and degradation of fructose 2,6-bisphosphate. Endogenously generated adenosine cooperates with bacterial components to increase PFKFB3 isozyme activity, resulting in greater fructose 2,6-bisphosphate accumulation. PFKFB3 is required for increased growth, metabolic activity and is regulated through active JAK2 and STAT5.

Publikationen

Species	Application	Title
mouse	WB	Nature; FGF-dependent metabolic control of vascular development.; Authors - Pengchun Yu
human	WB	Cell; Role of PFKFB3-Driven Glycolysis in Vessel Sprouting.; Authors - De Bock Katrien K
human	WB	Cell Metab; CircACC1 Regulates Assembly and Activation of AMPK Complex under Metabolic Stress.; Authors - Qidong Li
human	WB,IP	Cell Metab; NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis.; Authors - Mi Kyung Park
mouse	WB	Circulation; Exercise-Induced Changes in Glucose Metabolism Promote Physiologic Cardiac Growth.; Authors - Andrew A Gibb
mouse	WB	ACS Nano; Dual Inhibition of Endoplasmic Reticulum Stress and Oxidation Stress Manipulates the Polarization of Macrophages under Hypoxia to Sensitize Immunotherapy.; Authors - Mengshi Jiang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Balawant (Verified Customer) (08-24-2022)

this antibody is working good for westerner blot.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: colon tissue and colon cancer cell line

FH

Balawant (Verified Customer) (05-08-2022)

tis antibody is working great at 1:2000 diluton. I have used this antibody from total cells lysate of colonic epithelial cells form human origin.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Colonic epithelial cells

FH

Iram (Verified Customer) (04-13-2022)

Very good signal

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Colon cancer cells

FH

Susmita (Verified Customer) (03-11-2022)

This antibody works great for me.

• Applications: Western Blot
• Primary Antibody Dilution: WB: 1: 2000
• Cell Tissue Type: PC cell line

FH

Hsin-Sheng (Verified Customer) (08-19-2019)

Strong bend intensity at around 60 kD. However, there is a non-specific band near 65-70 kD.

• Applications: Western Blot,
• Primary Antibody Dilution: 1;200
• Cell Tissue Type: H1299

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