P21 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

P21 Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (4)

Anwendung

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

755

Rezensionen (5)

Kat-Nr. : 10355-1-AP

Synonyme

CDKN1A, P21;CDKN1A, CDK-interacting protein 1, MDA-6, Cyclin-dependent kinase inhibitor 1

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Galerie der Validierungsdaten

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  WB analysis using 10355-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10355-1-AP (P21 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10355-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10355-1-AP (P21 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10355-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10355-1-AP (P21 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10355-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10355-1-AP (P21 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10355-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10355-1-AP (P21 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10355-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10355-1-AP (P21 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 10355-1-AP

  IP result of anti-P21 (IP:10355-1-AP, 4ug; Detection:10355-1-AP 1:1000) with MCF-7 cells lysate 1085 ug.

• 

  IHC staining of human cervical cancer using 10355-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10355-1-AP (P21;CDKN1A Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 10355-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10355-1-AP (P21;CDKN1A Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10355-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10355-1-AP (P21;CDKN1A antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10355-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10355-1-AP (P21;CDKN1A antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 10355-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10355-1-AP (P21;CDKN1A antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 10355-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10355-1-AP (P21;CDKN1A antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 10355-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10355-1-AP (P21;CDKN1A antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 10355-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10355-1-AP (P21;CDKN1A antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10355-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10355-1-AP (P21;CDKN1A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10355-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10355-1-AP (P21;CDKN1A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 10355-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10355-1-AP (P21 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 10355-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10355-1-AP (P21 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of myotome of a somite at E11.5 tissue using 10355-1-AP

  IF result of anti-p21 (CDKN1A, 10355-1-AP) in the myotome of a somite at E11.5 by Dr. Zalc A. and Dr. Relaix F. (Red = p21; Green=Pax3 from a GFP reporter; Blue=DAPI).

• 

  IF Staining of MCF-7 using 10355-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using P21 antibody (10355-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of MCF-7 using 10355-1-AP

  Immunofluorescent analysis of MCF-7 cells using 10355-1-AP (P21;CDKN1A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of MCF-7 using 10355-1-AP

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human P21 (10355-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HUVEC-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, LNCaP-Zellen, MCF-7-Zellen, NCCIT-Zellen, THP-1-Zellen, U2OS-Zellen
• Erfolgreiche IP: MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe, humanes Kolonkarzinomgewebe, humanes Magenkrebsgewebe, humanes Mammakarzinomgewebe, humanes Nierengewebe, humanes Schilddrüsenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Myotom eines Somiten bei E11.5-Gewebe
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen, Myotom eines Somiten bei E11.5-Gewebe
• Erfolgreiche Detektion in FC (Intra): MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:600
• Immunfluoreszenz (IF)-P: IF-P : 1:10-1:100
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 12 publications below
• WB: See 701 publications below
• IHC: See 62 publications below
• IF: See 49 publications below
• IP: See 2 publications below
• FC: See 1 publications below
• CoIP: See 2 publications below

Produktinformation

10355-1-AP bindet in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA P21 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Hausschwein, Hund, Kaninchen, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: P21 fusion protein Ag0368
• Vollständiger Name: cyclin-dependent kinase inhibitor 1A (p21, Cip1)
• Berechnetes Molekulargewicht: 18 kDa
• Beobachtetes Molekulargewicht: 21 kDa
• GenBank-Zugangsnummer: BC000275
• Gene symbol: p21
• Gene ID (NCBI): 1026
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CDKN1A (p21, CIP1, WAF1) is a cyclin-dependent kinase inhibitor. CDKN1A binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at the G1 phase. The expression of CDKN1A is induced by wild-type but not mutant p53 protein, through which CDKN1A mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. CDKN1A can interact with proliferating cell nuclear antigen (PCNA), and plays a regulatory role in S phase DNA replication and DNA damage repair. CDKN1A was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported.

Publikationen

Species	Application	Title
human	WB,IHC	Gastroenterology; Intestinal PPARα Protects Against Colon Carcinogenesis via Regulation of Methyltransferases DNMT1 and PRMT6.; Authors - Yuhong Luo
human	WB	Sci Transl Med; Guanosine diphosphate-mannose suppresses homologous recombination repair and potentiates antitumor immunity in triple-negative breast cancer; Authors - Jia-Han Ding
human	WB	Nat Commun; Senescence-associated 13-HODE production promotes age-related liver steatosis by directly inhibiting catalase activity; Authors - Jinjie Duan
human	WB	Nat Commun; URI alleviates tyrosine kinase inhibitors-induced ferroptosis by reprogramming lipid metabolism in p53 wild-type liver cancers; Authors - Zhiwen Ding
human	WB	Nat Commun; Phosphoglycerate dehydrogenase activates PKM2 to phosphorylate histone H3T11 and attenuate cellular senescence; Authors - Yinsheng Wu
human	WB	Mol Cancer; LncRNA TROJAN promotes proliferation and resistance to CDK4/6 inhibitor via CDK2 transcriptional activation in ER+ breast cancer.; Authors - Xi Jin

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Vikas (Verified Customer) (04-23-2024)

Used p21 antibody at 1:1000 dilution for western blotting worked great. Thank You. Highly recommended.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: SH-SY5Y cell line

FH

Hadil (Verified Customer) (05-24-2023)

I prepared this antibody in odyssey blocker at 1:1000 and incubated at 4 C overnight and seemed to get a good signal for p21. There were some non-specific bands properly caused by long incubation periods. So, i would recommend using it at this concentration for 1.5-2 hr at RT.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mammalian cell lines

FH

Malak (Verified Customer) (04-22-2021)

The best incubation period for this antibody is 1.5 hr at room temperature, longer incubations (especially overnight at 4°C) may result in aspecific bands.

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Hepatic cancer cell extract

FH

Susan (Verified Customer) (12-03-2019)

We had been having issues with our old P21 antibody and this one worked great.

• Applications: Western Blot,
• Cell Tissue Type: keratinocytes, dog

FH

Béatrice (Verified Customer) (09-04-2019)

This is an excellent and specific antibody, without background.When prepared in 3%BSA, it can be reused several times and kept at-20°C

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HCT116

P21 Antibody Western Blot, validation (1/1000 dilution) in HCT116 (Cat no:10355-1-AP)

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