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c-MYC Polyclonal antibody
c-MYC Polyclonal Antibody for FC, IF, IP, WB, ELISA
Cat no : 10828-1-AP
Validation Data Gallery
|Positive WB detected in||HeLa cells, HT-29 cells, HL-60 cells, human placenta tissue, A549 cells, Jurkat cells, Raji cells, SH-SY5Y cells, HepG2 cells, Jurkat cells and Raji cells.|
|Positive IP detected in||MCF-7 cells|
|Positive IF detected in||Saos-2 cells, HEK-293 cells, U2OS cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:2000-1:12000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
10828-1-AP targets c-MYC in WB, IP, IF, FC, EMSA, CoIP, ChIP, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, mouse, rat, chicken, sheep, P. lilacinum, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||c-MYC fusion protein Ag1263|
|Full Name||v-myc myelocytomatosis viral oncogene homolog (avian)|
|Calculated molecular weight||49 kDa|
|Observed molecular weight||62-65 kDa, 50 kDa|
|GenBank accession number||BC000141|
|Gene ID (NCBI)||4609|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
c-Myc (also known as Myc), together with l-Myc and n-Myc, belongs to the Myc family of transcription factors. c-Myc has a basic helix-loop-helix leucine zipper domain and through heterodimerization can bind and regulate the transcriptional activity of genes, either by repression or activation. It is a key player in the regulation of cell growth and cell cycle progression and acts as a proto-oncogene.
c-Myc is ubiquitously expressed in almost all cell types and its expression positively correlates with tissue proliferative capacity. c-Myc is also expressed during embryogenesis and is upregulated in many cancer types.
Involvement in disease
· Upregulated in many cancer types, especially in aggressive, poorly differentiated tumors.
· Mutations in the MYC gene and breakpoint translocations within the MYC gene cause Burkitt lymphoma.
There are 3 different isoforms of c-Myc: c-Myc1, c-Myc2, and c-MycS (PMID: 16260605). They differ in molecular size, can be preferentially expressed during cell growth, and are reported to be functionally distinct.
c-Myc is subject to various post-translational modifications, including phosphorylation, acetylation, and ubiquitinylation (PMID: 16987807), which regulate its activity.
c-Myc localizes to the nucleus but can also be present in the cytoplasm of certain cancer types.
RIC-seq for global in situ profiling of RNA-RNA spatial interactions.
Signal Transduct Target Ther
Overexpression of CIP2A is associated with poor prognosis in multiple myeloma.
Nucleolar HEATR1 upregulated by mTORC1 signaling promotes hepatocellular carcinoma growth by dominating ribosome biogenesis and proteome homeostasis
p53 Is a Master Regulator of Proteostasis in SMARCB1-Deficient Malignant Rhabdoid Tumors.
Efferocytosis induces macrophage proliferation to help resolve tissue injury.
Nat Cell Biol
Myc and Dnmt1 impede the pluripotent to totipotent state transition in embryonic stem cells.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Molly (Verified Customer) (05-02-2023)
The antibody worked well for western blotting following the recommended protocol.
Anna-Maria (Verified Customer) (08-13-2019)
This antibody worked as expected for western blotting
Christine (Verified Customer) (11-13-2018)
The antibody worked better than the Abcam c-Myc antibody. However, I did not get clear nuclear staining but rather a mix of nuclear staining with cytoplasmic staining. This could be due non-optimized conditions.
Claudio (Verified Customer) (12-14-2017)
We tested the antibody in IHC/IFL on mouse brain tumors that showed c-myc upregulation at the mRNA level.