c-MYC Polyclonal antibody

• Featured Product
• KD/KO Validated

c-MYC Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (6)

Applications

WB, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA, EMSA

Conjugate

Unconjugated

Publications

677

Reviews (4)

Cat no : 10828-1-AP

Synonyms

MYC, Myc proto oncogene protein, MRTL, Class E basic helix-loop-helix protein 39, c Myc

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Validation Data Gallery

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  WB analysis of A549 using 10828-1-AP

  WB result of c-MYC antibody (10828-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-c-MYC transfected A549 cells.

• 

  WB analysis using 10828-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10828-1-AP (c-MYC antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10828-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10828-1-AP (c-MYC antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10828-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10828-1-AP (c-MYC antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10828-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10828-1-AP (c-MYC antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 10828-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 10828-1-AP (c-MYC antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 10828-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 10828-1-AP (c-MYC antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 10828-1-AP

  IP result of anti-c-MYC (IP:10828-1-AP, 4ug; Detection:10828-1-AP 1:1000) with MCF-7 cells lysate 1660 ug.

• 

  IHC staining of human ovary cancer using 10828-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 10828-1-AP (c-MYC antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of U2OS using 10828-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using c-MYC antibody (10828-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of Saos-2 using 10828-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Saos-2 cells using c-MYC antibody (10828-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of Saos-2 using 10828-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Saos-2 cells using c-MYC antibody (10828-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of HEK-293 using 10828-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using c-MYC antibody (10828-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  FC experiment of HeLa using 10828-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human c-MYC (10828-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive WB detected in: HT-29 cells, Jurkat cells, HeLa cells, A549 cells, HL-60 cells, human placenta tissue, Raji cells, SH-SY5Y cells, HepG2 cells, BxPC-3 cells, SW 1990 cells, Jurkat cells and Raji cells.
• Positive IP detected in: MCF-7 cells
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Saos-2 cells, HEK-293 cells, U2OS cells
• Positive FC (Intra) detected in: HeLa cells

This antibody is not for the detetion of MYC-tag.

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 25 publications below
• WB: See 615 publications below
• IF: See 41 publications below
• IP: See 24 publications below
• CoIP: See 15 publications below
• ChIP: See 14 publications below

Product Information

10828-1-AP targets c-MYC in WB, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA, EMSA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat, pig, chicken, sheep, p. lilacinum
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: c-MYC fusion protein Ag1263
• Full Name: v-myc myelocytomatosis viral oncogene homolog (avian)
• Calculated Molecular Weight: 49 kDa
• Observed Molecular Weight: 62-65 kDa, 50 kDa
• GenBank Accession Number: BC000141
• Gene Symbol: MYC
• Gene ID (NCBI): 4609
• RRID: AB_2148585
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Function

c-Myc (also known as Myc), together with l-Myc and n-Myc, belongs to the Myc family of transcription factors. c-Myc has a basic helix-loop-helix leucine zipper domain and through heterodimerization can bind and regulate the transcriptional activity of genes, either by repression or activation. It is a key player in the regulation of cell growth and cell cycle progression and acts as a proto-oncogene.

Tissue specificity

c-Myc is ubiquitously expressed in almost all cell types and its expression positively correlates with tissue proliferative capacity. c-Myc is also expressed during embryogenesis and is upregulated in many cancer types.

Involvement in disease

·         Upregulated in many cancer types, especially in aggressive, poorly differentiated tumors.

·         Mutations in the MYC gene and breakpoint translocations within the MYC gene cause Burkitt lymphoma.

Isoforms

There are 3 different isoforms of c-Myc: c-Myc1, c-Myc2, and c-MycS (PMID: 16260605). They differ in molecular size, can be preferentially expressed during cell growth, and are reported to be functionally distinct. The 50kDa band recognized by antibody is the native form of MYC, while the other bands, between 60-70kDa, are the phosphorylated form of MYC (PMID: 12189186).

Post-translational modifications

c-Myc is subject to various post-translational modifications, including phosphorylation, acetylation, and ubiquitinylation (PMID: 16987807), which regulate its activity.

Cellular localization

c-Myc localizes to the nucleus but can also be present in the cytoplasm of certain cancer types.

Publications

Species	Application	Title
human	WB	Nature; RIC-seq for global in situ profiling of RNA-RNA spatial interactions.; Authors - Zhaokui Cai
mouse	WB	Signal Transduct Target Ther; MDIG-mediated H3K9me3 demethylation upregulates Myc by activating OTX2 and facilitates liver regeneration; Authors - Jinpeng Du ; KD Validated
human	WB	Signal Transduct Target Ther; Overexpression of CIP2A is associated with poor prognosis in multiple myeloma.; Authors - Xuewen Liu
human	WB,IF	Gastroenterology; Nucleolar HEATR1 upregulated by mTORC1 signaling promotes hepatocellular carcinoma growth by dominating ribosome biogenesis and proteome homeostasis; Authors - Xiao-Mei Yang
human	WB	Cancer Cell; p53 Is a Master Regulator of Proteostasis in SMARCB1-Deficient Malignant Rhabdoid Tumors.; Authors - Alessandro Carugo ; KD Validated
	WB	Cell Metab; Efferocytosis induces macrophage proliferation to help resolve tissue injury.; Authors - Brennan D Gerlach ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Molly (Verified Customer) (05-02-2023)

The antibody worked well for western blotting following the recommended protocol.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Ewing sarcoma cell lines

FH

Anna-Maria (Verified Customer) (08-13-2019)

This antibody worked as expected for western blotting

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Bladder cancer cell lines

FH

Christine (Verified Customer) (11-13-2018)

The antibody worked better than the Abcam c-Myc antibody. However, I did not get clear nuclear staining but rather a mix of nuclear staining with cytoplasmic staining. This could be due non-optimized conditions.

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1 to 300
• Cell Tissue Type: Colon

FH

Claudio (Verified Customer) (12-14-2017)

We tested the antibody in IHC/IFL on mouse brain tumors that showed c-myc upregulation at the mRNA level.

• Applications: Immunohistochemistry, Immunofluorescence
• Primary Antibody Dilution: 100
• Cell Tissue Type: Mouse brain tumors with c-myc upregulation (determined at the mRNA level)

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