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  • KD/KO Validated

c-MYC Polyclonal antibody

c-MYC Polyclonal Antibody for WB, IP, IF, FC, ELISA

Host / Isotype

Rabbit / IgG


human and More (6)





Cat no : 10828-1-AP


bHLHe39, c Myc, c-Myc, MRTL, MYC, Myc proto oncogene protein, Proto oncogene c Myc, Transcription factor p64

Tested Applications

Positive WB detected inHT-29 cells, Jurkat cells, HeLa cells, A549 cells, HL-60 cells, human placenta tissue, Raji cells, SH-SY5Y cells, HepG2 cells, BxPC-3 cells, SW 1990 cells, Jurkat cells and Raji cells.
Positive IP detected inMCF-7 cells
Positive IF detected inSaos-2 cells, HEK-293 cells, U2OS cells
Positive FC detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10828-1-AP targets c-MYC in WB, IP, IF, FC, EMSA, CoIP, chIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat, chicken, sheep, P. lilacinum, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen c-MYC fusion protein Ag1263
Full Name v-myc myelocytomatosis viral oncogene homolog (avian)
Calculated Molecular Weight 49 kDa
Observed Molecular Weight 62-65 kDa, 50 kDa
GenBank Accession NumberBC000141
Gene Symbol MYC
Gene ID (NCBI) 4609
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information


c-Myc (also known as Myc), together with l-Myc and n-Myc, belongs to the Myc family of transcription factors. c-Myc has a basic helix-loop-helix leucine zipper domain and through heterodimerization can bind and regulate the transcriptional activity of genes, either by repression or activation. It is a key player in the regulation of cell growth and cell cycle progression and acts as a proto-oncogene.

Tissue specificity

c-Myc is ubiquitously expressed in almost all cell types and its expression positively correlates with tissue proliferative capacity. c-Myc is also expressed during embryogenesis and is upregulated in many cancer types.

Involvement in disease

·         Upregulated in many cancer types, especially in aggressive, poorly differentiated tumors.

·         Mutations in the MYC gene and breakpoint translocations within the MYC gene cause Burkitt lymphoma.


There are 3 different isoforms of c-Myc: c-Myc1, c-Myc2, and c-MycS (PMID: 16260605). They differ in molecular size, can be preferentially expressed during cell growth, and are reported to be functionally distinct.

Post-translational modifications

c-Myc is subject to various post-translational modifications, including phosphorylation, acetylation, and ubiquitinylation (PMID: 16987807), which regulate its activity.

Cellular localization

c-Myc localizes to the nucleus but can also be present in the cytoplasm of certain cancer types.


Product Specific Protocols
WB protocol for c-MYC antibody 10828-1-APDownload protocol
IF protocol for c-MYC antibody 10828-1-APDownload protocol
IP protocol for c-MYC antibody 10828-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




RIC-seq for global in situ profiling of RNA-RNA spatial interactions.

Authors - Zhaokui Cai

Signal Transduct Target Ther

MDIG-mediated H3K9me3 demethylation upregulates Myc by activating OTX2 and facilitates liver regeneration

Authors - Jinpeng Du
  • KD Validated

Signal Transduct Target Ther

Overexpression of CIP2A is associated with poor prognosis in multiple myeloma.

Authors - Xuewen Liu


Nucleolar HEATR1 upregulated by mTORC1 signaling promotes hepatocellular carcinoma growth by dominating ribosome biogenesis and proteome homeostasis

Authors - Xiao-Mei Yang

Cancer Cell

p53 Is a Master Regulator of Proteostasis in SMARCB1-Deficient Malignant Rhabdoid Tumors.

Authors - Alessandro Carugo
  • KD Validated

Cell Metab

Efferocytosis induces macrophage proliferation to help resolve tissue injury.

Authors - Brennan D Gerlach
  • KD Validated


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Molly (Verified Customer) (05-02-2023)

The antibody worked well for western blotting following the recommended protocol.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Ewing sarcoma cell lines

Anna-Maria (Verified Customer) (08-13-2019)

This antibody worked as expected for western blotting

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Bladder cancer cell lines

Christine (Verified Customer) (11-13-2018)

The antibody worked better than the Abcam c-Myc antibody. However, I did not get clear nuclear staining but rather a mix of nuclear staining with cytoplasmic staining. This could be due non-optimized conditions.

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1 to 300
  • Cell Tissue Type: Colon

Claudio (Verified Customer) (12-14-2017)

We tested the antibody in IHC/IFL on mouse brain tumors that showed c-myc upregulation at the mRNA level.

  • Applications: Immunohistochemistry, Immunofluorescence
  • Primary Antibody Dilution: 100
  • Cell Tissue Type: Mouse brain tumors with c-myc upregulation (determined at the mRNA level)