Osteocalcin Polyklonaler Antikörper

Osteocalcin Polyklonal Antikörper für FC, IF, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

151

Rezensionen (4)

Kat-Nr. : 23418-1-AP

Synonyme

BGLAP, Osteocalcin, BGP, Bone Gla protein, OC, OCN, Osteocalcin, PMF1

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Galerie der Validierungsdaten

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  IHC staining of mouse testis using 23418-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 23418-1-AP (Osteocalcin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 23418-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 23418-1-AP (Osteocalcin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human osteosarcoma using 23418-1-AP

  Immunohistochemical analysis of paraffin-embedded human osteosarcoma tissue slide using 23418-1-AP (Osteocalcin Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human osteosarcoma using 23418-1-AP

  Immunohistochemical analysis of paraffin-embedded human osteosarcoma tissue slide using 23418-1-AP (Osteocalcin Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 23418-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 23418-1-AP (Osteocalcin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 23418-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 23418-1-AP (Osteocalcin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 23418-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 23418-1-AP (Osteocalcin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human osteosarcoma using 23418-1-AP

  Immunohistochemical analysis of paraffin-embedded human osteosarcoma slide using 23418-1-AP (Osteocalcin Antibody) at dilution of 1:50.

• 

  IHC staining of human osteosarcoma using 23418-1-AP

  Immunohistochemical analysis of paraffin-embedded human osteosarcoma slide using 23418-1-AP (Osteocalcin Antibody) at dilution of 1:50.

• 

  IHC staining of human osteosarcoma using 23418-1-AP

  Immunohistochemical analysis of paraffin-embedded human osteosarcoma tissue slide using 23418-1-AP (Osteocalcin Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human osteosarcoma using 23418-1-AP

  Immunohistochemical analysis of paraffin-embedded human osteosarcoma tissue slide using 23418-1-AP (Osteocalcin Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of rat testis using 23418-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 23418-1-AP (Osteocalcin Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 23418-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 23418-1-AP (Osteocalcin Antibody) at dilution of 1:50.

• 

  IHC staining of human testis using 23418-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 23418-1-AP (Osteocalcin Antibody) at dilution of 1:50.

• 

  IF Staining of U2OS using 23418-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using Osteocalcin antibody (23418-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of NIH/3T3 using 23418-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using Osteocalcin antibody (23418-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of NIH/3T3 using 23418-1-AP

  1X10^6 NIH/3T3 cells were intracellularly stained with 0.4 ug Anti-Human Osteocalcin (23418-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: Maushodengewebe, humanes Hodengewebe, humanes Osteosarkomgewebe, Mausnierengewebe, Rattenhodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: U2OS-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in FC: NIH/3T3-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 64 publications below
• IF: See 66 publications below

Produktinformation

23418-1-AP bindet in IHC, IF, FC, ELISA Osteocalcin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Hund, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Osteocalcin fusion protein Ag20065
• Vollständiger Name: bone gamma-carboxyglutamate (gla) protein
• Berechnetes Molekulargewicht: 100 aa, 11 kDa
• GenBank-Zugangsnummer: BC113432
• Gene symbol: BGLAP
• Gene ID (NCBI): 632
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Osteocalcin is a small, highly conserved molecule associated with mineralization of bone matrix. Osteocalcin is specifically expressed in osteoblasts and is the most abundant non-collagenous protein in bone. It regulates the dynamics of new bone formation and bone resorption by interaction with vitamin D, and by influencing the differentiation of osteoblasts. Osteocalcin is also involved in the posttranslational targeting of vitamin K-dependent gamma-carboxylation, which controls blood coagulation.

Publikationen

Species	Application	Title
mouse	IHC,IF	ACS Nano; Nanotopography Sequentially Mediates Human Mesenchymal Stem Cell-Derived Small Extracellular Vesicles for Enhancing Osteogenesis.; Authors - Liang Ma
mouse	IF	Biomaterials; A nano-conductive osteogenic hydrogel to locally promote calcium influx for electro-inspired bone defect regeneration; Authors - Congcong Yu
mouse	IF	Sci Adv; Targeting actin-bundling protein L-plastin as an anabolic therapy for bone loss.; Authors - Xiaoqun Li
mouse	IHC	Bone Res; HuR-mediated nucleocytoplasmic translocation of HOTAIR relieves its inhibition of osteogenic differentiation and promotes bone formation; Authors - Yuheng Li
human	IHC	Nat Commun; Small extracellular vesicle-mediated miR-320e transmission promotes osteogenesis in OPLL by targeting TAK1.; Authors - Chen Xu
mouse	IF	J Clin Invest; SIRT6 protects vascular smooth muscle cells from osteogenic transdifferentiation via Runx2 in chronic kidney disease.; Authors - Wenxin Li

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

CHAO (Verified Customer) (03-31-2022)

Weak bands around the correct size

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse Heart

FH

LUNFENG (Verified Customer) (01-27-2020)

GOOD

• Applications: Immunofluorescence,
• Cell Tissue Type: HEART

FH

Jie (Verified Customer) (01-23-2020)

Not working very well in my western blot

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse kidney tissue

FH

Canzhao (Verified Customer) (01-17-2020)

This antibody worked very well on western blot and immunofluorescence, very specific. I recommend this antibody!

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:1000 for WB, 1:100 for IF
• Cell Tissue Type: Mouse heart and cultured cardiomyocytes.

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