Cytokeratin 18 Polyklonaler Antikörper

Cytokeratin 18 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (6)

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen

107

Rezensionen (1)

Kat-Nr. : 10830-1-AP

Synonyme

KRT18, keratin 18, Cytokeratin,Keratin, CK-18, CK18

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Galerie der Validierungsdaten

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  WB analysis using 10830-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 10830-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A431 using 10830-1-AP

  IP result of anti-Cytokeratin 18 (IP:10830-1-AP, 3ug; Detection:10830-1-AP 1:2000) with A431 cells lysate 3300ug.

• 

  IHC staining of human breast cancer using 10830-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10830-1-AP (Cytokeratin 18 antibody at dilution of 1:1000 (under 10x lens).

• 

  IHC staining of human breast cancer using 10830-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10830-1-AP (Cytokeratin 18 antibody at dilution of 1:1000 (under 40x lens).

• 

  IHC staining of human colon cancer using 10830-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10830-1-AP (Cytokeratin 18 antibody at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10830-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10830-1-AP (Cytokeratin 18 antibody at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate hyperplasia using 10830-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate hyperplasia using 10830-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 10830-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10830-1-AP (Cytokeratin 18 antibody at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 10830-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10830-1-AP (Cytokeratin 18 antibody at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 10830-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human stomach cancer using 10830-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human endometrial cancer using 10830-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human endometrial cancer using 10830-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung cancer using 10830-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10830-1-AP (Cytokeratin 18 antibody at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10830-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10830-1-AP (Cytokeratin 18 antibody at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 10830-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver cancer using 10830-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human pancreas cancer using 10830-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 10830-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary tumor using 10830-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary tumor using 10830-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 10830-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10830-1-AP (Cytokeratin 18 antibody at dilution of 1:1000 (under 10x lens).

• 

  IHC staining of human kidney using 10830-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10830-1-AP (Cytokeratin 18 antibody at dilution of 1:1000 (under 40x lens).

• 

  IF Staining of HepG2 using 10830-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Cytokeratin 18 antibody (10830-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 10830-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Cytokeratin 18 antibody (10830-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 10830-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.8 ug Cytokeratin 18 Polyclonal antibody (10830-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A431-Zellen, K-562-Zellen
• Erfolgreiche IP: A431-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Endometriumkarzinomgewebe, humanes Kolonkarzinomgewebe, humanes Leberkarzinomgewebe, humanes Lungenkarzinomgewebe, humanes Magenkrebsgewebe, humanes Nierengewebe, humanes Nierenzellkarzinomgewebe, humanes Ovarialkarzinomgewebe, humanes Pankreaskarzinomgewebe, humanes Prostatahyperplasie-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen, HeLa-Zellen, Maus-Riechepithelgewebe
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 36 publications below
• IHC: See 24 publications below
• IF: See 49 publications below
• FC: See 2 publications below

Produktinformation

10830-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA Cytokeratin 18 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Affe, Ente, Hausschwein, Maus, Ratte, Rind, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Cytokeratin 18 fusion protein Ag1260
• Vollständiger Name: keratin 18
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC000180
• Gene symbol: Cytokeratin 18
• Gene ID (NCBI): 3875
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT18, also named as CYK18, PIG46 and K18, is the most commonly found members of the intermediate filament gene family. KRT18 is a type I keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas.

Publikationen

Species	Application	Title
mouse	IHC	Cell Stem Cell; Injury Induces Endogenous Reprogramming and Dedifferentiation of Neuronal Progenitors to Multipotency.; Authors - Brian Lin
mouse	IF	Hepatology; Fat-associated Lymphoid Clusters as Expandable Niches for Ectopic Liver Development.; Authors - Bing Han
rat	IHC	J Control Release; Mesenchymal stem cell exosomes as nanotherapeutics for dry age-related macular degeneration; Authors - Yue Tang
rat	IF	Acta Biomater; A KPV-binding double-network hydrogel restores gut mucosal barrier in an inflamed colon.; Authors - Yingzheng Zhao
mouse	IHC,IF	Proc Natl Acad Sci U S A; Role of estrogen receptor beta in uterine stroma and epithelium: Insights from estrogen receptor beta-/- mice.; Authors - Wada-Hiraike Osamu O
mouse	IF	Proc Natl Acad Sci U S A; Basal epithelial stem cells are efficient targets for prostate cancer initiation.; Authors - Lawson Devon A DA

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Parmveer (Verified Customer) (12-02-2019)

Works well for both IHC and IF, but does come with a bit of background

• Applications: Immunohistochemistry, Immunofluorescence,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Prostate

Cytokeratin 18 Antibody Immunohistochemistry,Immunofluorescence, validation (1:1000 dilution) in Prostate (Cat no:10830-1-AP)

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