Tested Applications
| Positive WB detected in | human placenta tissue, mouse lung tissue, rat lung tissue |
| Positive IHC detected in | mouse heart tissue, human lung cancer tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11920-1-AP targets IL-1RL1/ST2 in WB, IHC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2555 Product name: Recombinant human IL-1RL1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 4-328 aa of BC030975 Sequence: WILAILTILMYSTAAKFSKQSWGLENEALIVRCPRQGKPSYTVDWYYSQTNKSIPTQERNRVFASGQLLKFLPAAVADSGIYTCIVRSPTFNRTGYANVTIYKKQSDCNVPDYLMYSTVSGSEKNSKIYCPTIDLYNWTAPLEWFKNCQALQGSRYRAHKSFLVIDNVMTEDAGDYTCKFIHNENGANYSVTATRSFTVKDEQGFSLFPVIGAPAQNEIKEVEIGKNANLTCSACFGKGTQFLAAVLWQLNGTKITDFGEPRIQQEEGQNQSFSNGLACLDMVLRIADVKEEDLLLQYDCLALNLHGLRRHTVRLSRKNPSKECF Predict reactive species |
| Full Name | interleukin 1 receptor-like 1 |
| Calculated Molecular Weight | 63 kDa, 37 kDa, 30 kDa |
| Observed Molecular Weight | 50 kDa |
| GenBank Accession Number | BC030975 |
| Gene Symbol | IL1RL1 |
| Gene ID (NCBI) | 9173 |
| RRID | AB_906359 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q01638 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ST2, also known as IL1RL1, is a member of the IL-1 superfamily and serves as the receptor for IL-33. It plays a major role in immune and inflammatory responses. ST2 is expressed by various types of immune cells including Th2 cells, mast cells, type 2 innate lymphoid cells, eosinophils, basophils, NK cells, and non-immune cells, including cardiomyocytes. Two forms of ST2 exist: a membrane-bound form and a soluble form (sST2). The membrane-bound form activates the MyD88/NF-κB signaling pathway to enhance mast cell, Th2, regulatory T cell (Treg), and innate lymphoid cell type 2 functions. sST2 acts as a decoy receptor. (PMID: 27055914; 23999434; 28484466)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for IL-1RL1/ST2 antibody 11920-1-AP | Download protocol |
| IHC protocol for IL-1RL1/ST2 antibody 11920-1-AP | Download protocol |
| WB protocol for IL-1RL1/ST2 antibody 11920-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-IL-33 antibody (Cat# 11920-1-AP) has accumulated 55 citations in journals including Cell, PNAS, Oncogene, J Neuroinflammation, Int Immunopharmacol, Sci Rep, Am J Pathol, Stem Cells, and Circ Heart Fail. Associated research fields include immunology and inflammation, cardiovascular disease, oncology, neurology, renal disease, respiratory disorders, autoimmune diseases, and hepatic fibrosis.
| Species | Application | Title |
|---|---|---|
Cell Charting the cellular biogeography in colitis reveals fibroblast trajectories and coordinated spatial remodeling | ||
Cell Mol Immunol Topical delivery of a human single-domain antibody targeting IL-33 to inhibit mucosal inflammation | ||
MedComm (2020) Partial reduction of interleukin-33 signaling improves senescence and renal injury in diabetic nephropathy | ||
Cell Death Dis Macrophages originated IL-33/ST2 inhibits ferroptosis in endometriosis via the ATF3/SLC7A11 axis
| ||
Cell Commun Signal IL-33/ST2 antagonizes STING signal transduction via autophagy in response to acetaminophen-mediated toxicological immunity | ||
J Neuroinflammation Red nucleus IL-33 facilitates the early development of mononeuropathic pain in male rats by inducing TNF-α through activating ERK, p38 MAPK, and JAK2/STAT3. |
