Desmoplakin Polyclonal antibody

• Featured Product
• KD/KO Validated

Desmoplakin Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

15

Cat no : 25318-1-AP

Synonyms

DSP, DP, DESP

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Validation Data Gallery

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  WB analysis of HepG2 using 25318-1-AP

  WB result of Desmoplakin antibody (25318-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Desmoplakin transfected HepG2 cells.

• 

  WB analysis using 25318-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25318-1-AP (Desmoplakin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 25318-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 25318-1-AP (Desmoplakin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of BxPC-3 using 25318-1-AP

  IP result of anti-Desmoplakin (IP:25318-1-AP, 4ug; Detection:25318-1-AP 1:10000) with BxPC-3 cells lysate 2080 ug.

• 

  IHC staining of mouse heart using 25318-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 25318-1-AP (Desmoplakin antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 25318-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 25318-1-AP (Desmoplakin antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human normal colon using 25318-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25318-1-AP (Desmoplakin antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human bowen disease using 25318-1-AP

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 25318-1-AP (Desmoplakin antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human bowen disease using 25318-1-AP

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 25318-1-AP (Desmoplakin antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A431 using 25318-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Desmoplakin antibody (25318-1-AP) at dilution of 1:1500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), Beta Actin antibody (66009-1-Ig, Clone: 2D4H5, red).

• 

  IF Staining of A431 using 25318-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Desmoplakin antibody (25318-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of A431 using 25318-1-AP

  1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human Desmoplakin (25318-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: A431 cells, HepG2 cells, mouse heart tissue, BxPC-3 cells, HEK-293 cells
• Positive IP detected in: BxPC-3 cells
• Positive IHC detected in: rat heart tissue, human normal colon, human bowen disease, mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells
• Positive FC (Intra) detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:2500-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:750-1:3000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 13 publications below
• IF: See 5 publications below
• IP: See 1 publications below

Product Information

25318-1-AP targets Desmoplakin in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Desmoplakin fusion protein Ag18065
• Full Name: desmoplakin
• Calculated Molecular Weight: 2871 aa, 332 kDa
• Observed Molecular Weight: 240-330 kDa
• GenBank Accession Number: BC140802
• Gene Symbol: DSP
• Gene ID (NCBI): 1832
• RRID: AB_2880028
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The desmoplakin (DP) is the most abundant desmosomal proteins and is located at the cytoplasmic portion of the desmosomes which are specialized cell-cell adhesion structures abundant in tissues such as muscle and epidermis that are subjected to mechanical stress. Desmoplakin 1 and 2 (DP 1 and 2) are two splice variants sharing common C-terminal and N-terminal globular head and tail domains, but differ in the length of the rod domain that links them. This antibody detects both DP1 (280-330 kDa) and DP2 (240-260 kDa). (PMID: 16467215, 11781569)

Publications

Species	Application	Title
mouse	WB	Bone Res; Overexpression of Lrp5 enhanced the anti-breast cancer effects of osteocytes in bone.; Authors - Shengzhi Liu
mouse	WB,IF	Theranostics; Cdc42 Deficiency Leads To Epidermal Barrier Dysfunction by Regulating Intercellular Junctions and Keratinization of Epidermal Cells during Mouse Skin Development.; Authors - Min Zhang
human	WB	Cancer Lett; ETV4 is a theranostic target in clear cell renal cell carcinoma that promotes metastasis by activating the pro-metastatic gene FOSL1 in a PI3K-AKT dependent manner.; Authors - Liang Xu
mouse	WB	Cancers (Basel); Inhibition of the Growth of Breast Cancer-Associated Brain Tumors by the Osteocyte-Derived Conditioned Medium.; Authors - Tomohiko Sano
human	WB	J Cell Mol Med; Wilms' tumour 1-associating protein inhibits endothelial cell angiogenesis by m6A-dependent epigenetic silencing of desmoplakin in brain arteriovenous malformation.; Authors - Lin-Jian Wang ; KD Validated
human	WB	Cell Death Discov; Long noncoding RNA LYPLAL1-AS1 regulates adipogenic differentiation of human mesenchymal stem cells by targeting desmoplakin and inhibiting the Wnt/β-catenin pathway.; Authors - Yanlei Yang ; KD Validated

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