Cytokeratin 8 Polyclonal antibody
Cytokeratin 8 Polyclonal Antibody for WB, IHC, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, IF, FC (Intra), ELISA
Conjugate
Unconjugated
Cat no : 17514-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A431 cells, A549 cells, C6 cells, HeLa cells, MCF-7 cells |
Positive IHC detected in | human stomach tissue, human bowen disease, human colon tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 8 publications below |
IHC | See 8 publications below |
IF | See 6 publications below |
Product Information
17514-1-AP targets Cytokeratin 8 in WB, IHC, IF, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, hamster |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide |
Full Name | keratin 8 |
Calculated Molecular Weight | 54 kDa |
Observed Molecular Weight | 54 kDa |
GenBank Accession Number | NM_002273 |
Gene Symbol | Cytokeratin 8 |
Gene ID (NCBI) | 3856 |
RRID | AB_2134726 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT8 is often paired with keratin 18 in vivo. This antibody is specifically against KRT8.
Protocols
Product Specific Protocols | |
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WB protocol for Cytokeratin 8 antibody 17514-1-AP | Download protocol |
IHC protocol for Cytokeratin 8 antibody 17514-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Oncogene The keratin 17/YAP/IL6 axis contributes to E-cadherin loss and aggressiveness of diffuse gastric cancer | ||
Clin Transl Med Aptamer-SH2 superbinder-based targeted therapy for pancreatic ductal adenocarcinoma. | ||
Sci Rep Conversion of embryonic stem cells into extraembryonic lineages by CRISPR-mediated activators. | ||
iScience Cysteine Dioxygenase Regulates the Epithelial Morphogenesis of Mammary Gland via Cysteine Sulfinic Acid. | ||
Regen Ther Dendrobium officinale polysaccharide promotes angiogenesis as well as follicle regeneration and hair growth through activation of the WNT signaling pathway | ||
Front Med (Lausanne) An Integrated Proteomics and Metabolomics Strategy for the Mechanism of Calcium Oxalate Crystal-Induced Kidney Injury. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Emma (Verified Customer) (11-29-2021) | Works very well by IF on FFPE tissue. Used at 1:50 with a Tris-EDTA antigen retrieval.
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FH Joleen (Verified Customer) (06-12-2019) | This antibody recognizes a band that matched with the expected molecular weight of KRT8.
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