Validation Data Gallery
|Positive WB detected in
|A431 cells, A549 cells, C6 cells, HeLa cells, MCF-7 cells
|Positive IHC detected in
|human breast cancer tissue, human prostate cancer tissue, human colon tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in
|Western Blot (WB)
|WB : 1:1000-1:8000
|IHC : 1:50-1:500
|Flow Cytometry (FC)
|FC : 0.20 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
17514-1-AP targets Cytokeratin 8 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, rat, mouse samples.
|human, rat, mouse
|human, mouse, hamster
|Host / Isotype
|Rabbit / IgG
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT8 is often paired with keratin 18 in vivo. This antibody is specifically against KRT8.
The keratin 17/YAP/IL6 axis contributes to E-cadherin loss and aggressiveness of diffuse gastric cancer
Clin Transl Med
Aptamer-SH2 superbinder-based targeted therapy for pancreatic ductal adenocarcinoma.
Conversion of embryonic stem cells into extraembryonic lineages by CRISPR-mediated activators.
Cysteine Dioxygenase Regulates the Epithelial Morphogenesis of Mammary Gland via Cysteine Sulfinic Acid.
Front Med (Lausanne)
An Integrated Proteomics and Metabolomics Strategy for the Mechanism of Calcium Oxalate Crystal-Induced Kidney Injury.
Cancer Gene Ther
Evaluation of apoptogenic adenovirus type 5 oncolytic vectors in a Syrian hamster head and neck cancer model.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Emma (Verified Customer) (11-29-2021)
Works very well by IF on FFPE tissue. Used at 1:50 with a Tris-EDTA antigen retrieval.
Joleen (Verified Customer) (06-12-2019)
This antibody recognizes a band that matched with the expected molecular weight of KRT8.