Cytokeratin 8 Polyclonal antibody

Cytokeratin 8 Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

18

Reviews (2)

Cat no : 17514-1-AP

Synonyms

KRT8, Cytokeratin-8, Cytokeratin,Keratin, CK-8, CK8

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Validation Data Gallery

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  WB analysis using 17514-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17514-1-AP (Cytokeratin 8 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 17514-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 17514-1-AP (Cytokeratin 8 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 17514-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 17514-1-AP (Cytokeratin 8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C6 using 17514-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 17514-1-AP (Cytokeratin 8 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 17514-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17514-1-AP (Cytokeratin 8 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 17514-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17514-1-AP (Cytokeratin 8 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human normal colon using 17514-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 17514-1-AP (Cytokeratin 8 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human normal colon using 17514-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 17514-1-AP (Cytokeratin 8 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach using 17514-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 17514-1-AP (Cytokeratin 8 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach using 17514-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 17514-1-AP (Cytokeratin 8 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 17514-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 17514-1-AP (Cytokeratin 8 antibody at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 17514-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 17514-1-AP (Cytokeratin 8 antibody at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 17514-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 17514-1-AP (Cytokeratin 8 antibody at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 17514-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 17514-1-AP (Cytokeratin 8 antibody at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of HeLa using 17514-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human Cytokeratin 8 (17514-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: A431 cells, A549 cells, C6 cells, HeLa cells, MCF-7 cells
• Positive IHC detected in: human breast cancer tissue, human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 8 publications below
• IHC: See 8 publications below
• IF: See 6 publications below

Product Information

17514-1-AP targets Cytokeratin 8 in WB, IHC, IF, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, hamster
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: keratin 8
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: NM_002273
• Gene Symbol: Cytokeratin 8
• Gene ID (NCBI): 3856
• RRID: AB_2134726
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT8 is often paired with keratin 18 in vivo. This antibody is specifically against KRT8.

Publications

Species	Application	Title
human	WB	Oncogene; The keratin 17/YAP/IL6 axis contributes to E-cadherin loss and aggressiveness of diffuse gastric cancer; Authors - Mengjie Li
human	WB	Clin Transl Med; Aptamer-SH2 superbinder-based targeted therapy for pancreatic ductal adenocarcinoma.; Authors - An-Dong Liu
mouse	IHC	Sci Rep; Conversion of embryonic stem cells into extraembryonic lineages by CRISPR-mediated activators.; Authors - Shu Wei
mouse	WB,IF	iScience; Cysteine Dioxygenase Regulates the Epithelial Morphogenesis of Mammary Gland via Cysteine Sulfinic Acid.; Authors - Jianjun Zhao
mouse	IF	Regen Ther; Dendrobium officinale polysaccharide promotes angiogenesis as well as follicle regeneration and hair growth through activation of the WNT signaling pathway; Authors - Yujin Zhang
mouse	IHC	Front Med (Lausanne); An Integrated Proteomics and Metabolomics Strategy for the Mechanism of Calcium Oxalate Crystal-Induced Kidney Injury.; Authors - Songyan Gao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Emma (Verified Customer) (11-29-2021)

Works very well by IF on FFPE tissue. Used at 1:50 with a Tris-EDTA antigen retrieval.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: Prostate

Cytokeratin 8 Antibody Immunofluorescence validation (1:50 dilution) in Prostate (Cat no:17514-1-AP)

FH

Joleen (Verified Customer) (06-12-2019)

This antibody recognizes a band that matched with the expected molecular weight of KRT8.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1k
• Cell Tissue Type: MDCK GII

Cytokeratin 8 Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:17514-1-AP)

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