IFNAR2 Polyklonaler Antikörper

IFNAR2 Polyklonal Antikörper für WB, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Rezensionen (1)

Kat-Nr. : 10522-1-AP

Synonyme

IFN-alpha binding protein, IFNABR, IFN R 2, IFN R, IFN alpha/beta receptor 2

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Galerie der Validierungsdaten

• 

  WB analysis using 10522-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10522-1-AP (IFNAR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of MCF-7 using 10522-1-AP

  1x10^6 MCF-7 cells were intracellularly stained with 0.8 ug IFNAR2 Polyclonal antibody (10522-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, K-562-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in FC (Intra): MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IP: See 1 publications below

Produktinformation

10522-1-AP bindet in WB, FC (Intra), IP, ELISA IFNAR2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Hausschwein
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IFNAR2 fusion protein Ag0191
• Vollständiger Name: interferon (alpha, beta and omega) receptor 2
• Berechnetes Molekulargewicht: 331 aa, 37 kDa
• Beobachtetes Molekulargewicht: 58-70 kDa
• GenBank-Zugangsnummer: BC002793
• Gene symbol: IFNAR2
• Gene ID (NCBI): 3455
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	EMBO Rep; Degradation of WTAP blocks antiviral responses by reducing the m 6 A levels of IRF3 and IFNAR1 mRNA; Authors - Yong Ge
human	WB,IP	J Virol; African swine fever virus pH240R enhances viral replication via inhibition of the type I IFN signaling pathway; Authors - Guangqiang Ye
human	WB	J Virol; Herpes simplex virus 1 UL36USP antagonizes type I IFN-mediated antiviral innate immunity.; Authors - Hui Yuan
pig	WB	PLoS Pathog; African swine fever virus pB318L, a trans-geranylgeranyl-diphosphate synthase, negatively regulates cGAS-STING and IFNAR-JAK-STAT signaling pathways; Authors - Xiaohong Liu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Angie (Verified Customer) (04-09-2024)

The antibody detected a band around 70 kD at dilution of 1:2000 incubated at room temperature for 2 hour followed by secondary antibody donkey-anti-rabbit (Alexa Fluor 800) at 1:20000 dilution, incubated for 1 hour at room temperature.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: A549, HEK293

IFNAR2 Antibody Western Blot validation (1:2000 dilution) in A549, HEK293 (Cat no:10522-1-AP)

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