IFN Alpha Monoclonal antibody

IFN Alpha Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

3

CloneNo.

4A2A11

Cat no : 66162-1-Ig

Synonyms

IFL, IFN, IFN ALPHA, IFN alpha 1/13, IFNA@, IFNA1, IFNA13, IFN-alpha, LeIF D

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Validation Data Gallery

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  WB analysis of Transfected HEK-293 using 66162-1-Ig

  WB result of Interferon alpha antibody (66162-1-Ig; 1:300; room temperature for 1.5 hours) with negative control and Interferon alpha overexpressed Transfected HEK-293 cells.

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  WB analysis of Recombinant protein using 66162-1-Ig

  Recombinant protein were subjected to SDS PAGE followed by western blot with 66162-1-Ig (Interferon alpha antibody at dilution of 1:40000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Recombinant protein, Transfected HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

66162-1-Ig targets IFN Alpha in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: IFN Alpha fusion protein Ag12533
• Full Name: IFNA1
• Calculated Molecular Weight: 189 aa, 22 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC074928
• Gene Symbol: IFN alpha1
• Gene ID (NCBI): 3439
• RRID: AB_2881558
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IFNA1 (IF-alpha) is a member of the Type I IFN (1) family best known for their antiviral activity. It is a key cytokine regulating the activity of B cells, T-helper cells (Th cells), cDCs and natural killer cells (NK cells). IFNA1 induces B cell maturation into plasma cells and immunoglobulin production. IFNA1 plays an important role in the pathogenesis of systemic lupus erythematosus (SLE). IFNA1 was the first cytokine to show clinical benefit in the treatment of certain types of cancer, including melanoma, chronic myelogenous leukemia, and renal cancer.

Publications

Species	Application	Title
human	WB	Amino Acids; Escherichia coli infection activates the production of IFN-α and IFN-β via the JAK1/STAT1/2 signaling pathway in lung cells.; Authors - Yan Jin
human	WB	Brain Res; Serum miR-221-3p as a new potential biomarker for depressed mood in perioperative patients.; Authors - Jianguo Feng
human	WB	Cell Rep; Nuclear translocation of cGAS orchestrates VEGF-A-mediated angiogenesis; Authors - Juanjuan Luo