STAT2 Polyclonal antibody

STAT2 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

17

Cat no : 16674-1-AP

Synonyms

ISGF 3, P113, STAT113, STAT2

---

Validation Data Gallery

• 

  WB analysis using 16674-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16674-1-AP (STAT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 16674-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 16674-1-AP (STAT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 16674-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 16674-1-AP (STAT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 16674-1-AP

  IP result of anti-STAT2 (IP:16674-1-AP, 4ug; Detection:16674-1-AP 1:500) with HeLa cells lysate 2800ug.

• 

  IHC staining of human breast cancer using 16674-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 16674-1-AP (STAT2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 16674-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 16674-1-AP (STAT2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung cancer using 16674-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 16674-1-AP (STAT2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 16674-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 16674-1-AP (STAT2 antibody) at dilution of 1:50 (under 40x lens).

---

Tested Applications

• Positive WB detected in: A431 cells, K-562 cells, HT-1080 cells, HeLa cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human breast cancer tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 16 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Product Information

16674-1-AP targets STAT2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: STAT2 fusion protein Ag10168
• Full Name: signal transducer and activator of transcription 2, 113kDa
• Calculated Molecular Weight: 851 aa, 98 kDa
• Observed Molecular Weight: 113 kDa
• GenBank Accession Number: BC051284
• Gene Symbol: STAT2
• Gene ID (NCBI): 6773
• RRID: AB_10644445
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

STAT2, also named as p113, belongs to the transcription factor STAT family. It is a signal transducer and activator of transcription that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN stimulated genes, which drive the cell in an antiviral state. It also interacts with CRSP2, CRSP6, Simian virus 5 protein V, rabies virus phosphoprotein, IFNAR1 and IFNAR2. Its interaction with dengue virus NS5 inhibits the phosphorylation of STAT2, and, when all viral proteins are present (polyprotein), STAT2 is targeted for degradation. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human STAT2. The calculated molecular weight of STAT2 is 98 kDa, but phosphorylated STAT2 is about 100-113 kDa.

Publications

Species	Application	Title
human	WB	Int J Mol Sci; A 2-Benzylmalonate Derivative as STAT3 Inhibitor Suppresses Tumor Growth in Hepatocellular Carcinoma by Upregulating β-TrCP E3 Ubiquitin Ligase.; Authors - Ting Peng
human	WB	Oncotarget; Dietary quercetin potentiates the antiproliferative effect of interferon-α in hepatocellular carcinoma cells through activation of JAK/STAT pathway signaling by inhibition of SHP2 phosphatase.; Authors - Ighodaro Igbe
pig	WB	Int J Biol Macromol; Atractylodis macrocephalae polysaccharides protect against DSS-induced intestinal injury through a novel lncRNA ITSN1-OT1.; Authors - Xin Zong
human	WB, IP, IF	J Immunol; Herpes Simplex Virus Type 2 Inhibits Type I IFN Signaling Mediated by the Novel E3 Ubiquitin Protein Ligase Activity of Viral Protein ICP22.; Authors - Mudan Zhang
human	WB	Toxicol Appl Pharmacol; PMID: 29031523; Authors - Orawan Wonganan
human	WB	Mol Cancer Res; STAT1 and NF-κB Inhibitors Diminish Basal Interferon-Stimulated Gene Expression and Improve the Productive Infection of Oncolytic HSV in MPNST Cells.; Authors - Joshua D Jackson