IFN Alpha Polyclonal antibody

IFN Alpha Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 18013-1-AP

Synonyms

IFL, IFN, IFN ALPHA, IFN alpha 1/13, IFNA@, IFNA1, IFNA13, IFN-alpha, Interferon alpha 1/13, Interferon alpha D, interferon, alpha 1, Interferon-α1, LeIF D

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Validation Data Gallery

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  WB analysis of Recombinant protein using 18013-1-AP

  Recombinant protein were subjected to SDS PAGE followed by western blot with 18013-1-AP (Interferon alpha antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Recombinant protein

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IHC: See 4 publications below
• IF: See 2 publications below

Product Information

The immunogen of 18013-1-AP is IFN Alpha Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IFN Alpha fusion protein Ag12533
• Full Name: IFNA1
• Calculated Molecular Weight: 189 aa, 22 kDa
• GenBank Accession Number: BC074928
• Gene Symbol: IFN alpha1
• Gene ID (NCBI): 3439
• RRID: AB_2878481
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IFNA1 (IFN-alpha) is a member of the Type I IFN (1) family best known for their antiviral activity. It is a key cytokine regulating the activity of B cells, T-helper cells (Th cells), cDCs and natural killer cells (NK cells). IFN-alpha induces B cell maturation into plasma cells and immunoglobulin production. IFN-alpha plays an important role in the pathogenesis of systemic lupus erythematosus (SLE). IFN-alpha was the first cytokine to show clinical benefit in the treatment of certain types of cancer, including melanoma, chronic myelogenous leukemia, and renal cancer.

Publications

Species	Application	Title
human	IHC	J Immunother Cancer; Helicobacter pylori infection disturbs the tumor immune microenvironment and is associated with a discrepant prognosis in gastric de novo diffuse large B-cell lymphoma.; Authors - Yuwei Deng
human		mBio; Early enterovirus translation deficits extend viral RNA replication and elicit sustained MDA5-directed innate signaling; Authors - Mikhail I Dobrikov
human	IF	Ann Allergy Asthma Immunol; Lower viral loads in subjects with rhinovirus-challenged allergy despite reduced innate immunity.; Authors - Xin Feng
human	WB	Dig Liver Dis; Innate and adaptive immunity in self-reported nonceliac gluten sensitivity versus celiac disease.; Authors - Antonio Di Sabatino
human	IHC,IF	Clin Oral Investig; Hypermethylation of IFN-γ in oral cancer tissues.; Authors - Songbo Tian
human	WB	Lung; Anti-inflammatory Properties of the Alpha-Melanocyte-Stimulating Hormone in Models of Granulomatous Inflammation.; Authors - Abdolrazagh Hashemi Shahraki