JAK1 Monoclonal antibody

• Featured Product
• KD/KO Validated

JAK1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, rat, mouse

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

54

CloneNo.

3H7A8

Reviews (1)

Cat no : 66466-1-Ig

Synonyms

JAK 1, JAK1, JAK1A, JAK1B, Janus kinase 1, JTK3, Tyrosine protein kinase JAK1

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Validation Data Gallery

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  WB analysis using 66466-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66466-1-Ig (JAK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

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  WB analysis using 66466-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66466-1-Ig (JAK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat spleen using 66466-1-Ig

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 66466-1-Ig (JAK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 66466-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66466-1-Ig (JAK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66466-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66466-1-Ig (JAK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 66466-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66466-1-Ig (JAK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 66466-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66466-1-Ig (JAK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: K-562 cells, Jurkat cells, rat spleen tissue, PC-12 cells, NIH/3T3 cells, RAW 264.7 cells, Ramos cells, Daudi cells
• Positive IHC detected in: human breast cancer tissue, human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 50 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

66466-1-Ig targets JAK1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, rat, mouse samples.

• Tested Reactivity: human, rat, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: JAK1 fusion protein Ag17940
• Full Name: Janus kinase 1 (a protein tyrosine kinase)
• Calculated Molecular Weight: 1154 aa, 133 kDa
• Observed Molecular Weight: 133 kDa
• GenBank Accession Number: BC132729
• Gene Symbol: JAK1
• Gene ID (NCBI): 3716
• RRID: AB_2881834
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Janus kinase 1(JAK1), is a member of a class of protein-tyrosine kinases (PTK) characterized by the presence of a second phosphotransferase-related domain immediately N-terminal to the PTK domain. JAK1 is essential for signaling for certain type I and type II cytokines. JAK1 plays a critical role in initiating responses to multiple major cytokine receptor families. Expression of JAK1 in cancer cells enables individual cells to contract, potentially allowing them to escape their tumor and metastasize to other parts of the body.

Publications

Species	Application	Title
human	IF	Adv Sci (Weinh); EHBP1L1 Drives Immune Evasion in Renal Cell Carcinoma through Binding and Stabilizing JAK1; Authors - Yihui Pan
mouse	WB	Cell Rep; Macrophage autophagy deficiency-induced CEBPB accumulation alleviates atopic dermatitis via impairing M2 polarization; Authors - Yongcheng Zhu
mouse	WB	Cell Chem Biol; Small-molecule MHC-II inducers promote immune detection and anti-cancer immunity via editing cancer metabolism; Authors - Ling Huang
mouse	WB	Antioxidants (Basel); Fibroblast Upregulation of Vitamin D Receptor Represents a Self-Protective Response to Limit Fibroblast Proliferation and Activation during Pulmonary Fibrosis; Authors - Juan Wei
human	WB	J Cell Mol Med; IGFBP7 regulates cell proliferation and migration through JAK/STAT pathway in gastric cancer and is regulated by DNA and RNA methylation; Authors - Weilie Mo
mouse	WB	Antioxidants (Basel); Periplaneta americana Extract Pretreatment Alleviates Oxidative Stress and Inflammation and Increases the Abundance of Gut Akkermansia muciniphila in Diquat-Induced Mice; Authors - Shiyi Lu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Christine (Verified Customer) (07-12-2022)

Signal is at the expected molecular weight, very low aspecific staining on the membrane, signal is proportional to the lysate quantity loaded. Need quite a large quantity of lysates.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: RPE1 cells and Huh7 cells (human)

JAK1 Antibody Western Blot validation (1:1000 dilution) in RPE1 cells and Huh7 cells (human) (Cat no:66466-1-Ig)