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IFITM1-Specific Monoklonaler Antikörper

IFITM1-Specific Monoklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

CloneNo.

5B5E2

Kat-Nr. : 60074-1-Ig

Synonyme

9 27, CD225, IFI17, IFITM1, IFITM1-Specific, Interferon induced protein 17, Leu 13 antigen, LEU13



Geprüfte Anwendungen

Erfolgreiche Detektion in WBTF-1-Zellen, HepG2-Zellen, HL-60-Zellen, humanes Hodengewebe, K-562-Zellen
Erfolgreiche IPHepG2-Zellen
Erfolgreiche Detektion in IHChumanes Lymphomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IFK-562-Zellen
Erfolgreiche Detektion in FCU2OS-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:20000-1:100000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:200-1:1000
Immunfluoreszenz (IF)IF : 1:200-1:800
Durchflusszytometrie (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

60074-1-Ig bindet in WB, IP, IHC, IF, FC, ELISA IFITM1-Specific und zeigt Reaktivität mit human

Getestete Reaktivität human
In Publikationen genannte Reaktivitäthuman, Maus
Wirt / Isotyp Maus / IgG2a
Klonalität Monoklonal
Typ Antikörper
Immunogen IFITM1-Specific fusion protein Ag2320
Vollständiger Name interferon induced transmembrane protein 1 (9-27)
Berechnetes Molekulargewicht 14 kDa
Beobachtetes Molekulargewicht 14-17 kDa
GenBank-ZugangsnummerBC000897
Gene symbol IFITM1
Gene ID (NCBI) 8519
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IFITM1(interferon induced transmembrane protein), also named DSPA2a and interferon-induced protein 17 (IFI17), belongs to the CD225 family. It has two transmembrane domain and serves as an IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, influenza A H1N1 virus, West Nile virus (WNV), and dengue virus , by inhibiting the early steps of replication. IFITM proteins are recently identified as viral restriction factors that inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. Also they serve as important components of the innate immune system to restrict HIV-1 infection. 60074-1-Ig is a mouse monoclonal antibody which specifically recognizing IFITM1 but not IFITM2 or IFITM3.

Protokolle

Produktspezifische Protokolle
WB protocol for IFITM1-Specific antibody 60074-1-IgProtokoll herunterladen
IHC protocol for IFITM1-Specific antibody 60074-1-IgProtokoll herunterladen
IF protocol for IFITM1-Specific antibody 60074-1-IgProtokoll herunterladen
IP protocol for IFITM1-Specific antibody 60074-1-IgProtokoll herunterladen
FC protocol for IFITM1-Specific antibody 60074-1-IgProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB,FC

Science

IFITM proteins inhibit placental syncytiotrophoblast formation and promote fetal demise.

Authors - Julian Buchrieser
humanWB

Cell

The IFITM proteins mediate cellular resistance to influenza A H1N1 virus, West Nile virus, and dengue virus.

Authors - Abraham L Brass
humanWB

Cell Stem Cell

PMID: 30416070

Authors - Carolina Petrillo
humanWB

Sci Transl Med

Dimethyl fumarate potentiates oncolytic virotherapy through NF-κB inhibition.

Authors - Mohammed Selman
humanWB

Cell Host Microbe

Resistance of Transmitted Founder HIV-1 to IFITM-Mediated Restriction.

Authors - Toshana L Foster
  • KD Validated
humanIF

Gastroenterology

PMID: 23123437

Authors - Daryl T-Y Lau

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Claudia (Verified Customer) (06-20-2022)

The antibody worked very well for the analysis of IFITM1 expression in permanent cell lines.

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1:400 (immunofluorescence), 1:20.000 (Western blot)
  • Cell Tissue Type: Permanent cell lines