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IFITM1-Specific Monoklonaler Antikörper
IFITM1-Specific Monoklonal Antikörper für FC, IF, IHC, IP, WB, ELISA
Wirt / Isotyp
Maus / IgG2a
Getestete Reaktivität
human und mehr (1)
Anwendung
WB, IP, IHC, IF, FC, ELISA
Konjugation
Unkonjugiert
CloneNo.
5B5E2
Kat-Nr. : 60074-1-Ig
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | TF-1-Zellen, HepG2-Zellen, HL-60-Zellen, humanes Hodengewebe, K-562-Zellen |
Erfolgreiche IP | HepG2-Zellen |
Erfolgreiche Detektion in IHC | humanes Lymphomgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Erfolgreiche Detektion in IF | K-562-Zellen |
Erfolgreiche Detektion in FC | U2OS-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:20000-1:100000 |
Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunhistochemie (IHC) | IHC : 1:200-1:1000 |
Immunfluoreszenz (IF) | IF : 1:200-1:800 |
Durchflusszytometrie (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
KD/KO | See 13 publications below |
WB | See 59 publications below |
IHC | See 3 publications below |
IF | See 11 publications below |
IP | See 1 publications below |
FC | See 3 publications below |
Produktinformation
60074-1-Ig bindet in WB, IP, IHC, IF, FC, ELISA IFITM1-Specific und zeigt Reaktivität mit human
Getestete Reaktivität | human |
In Publikationen genannte Reaktivität | human, Maus |
Wirt / Isotyp | Maus / IgG2a |
Klonalität | Monoklonal |
Typ | Antikörper |
Immunogen | IFITM1-Specific fusion protein Ag2320 |
Vollständiger Name | interferon induced transmembrane protein 1 (9-27) |
Berechnetes Molekulargewicht | 14 kDa |
Beobachtetes Molekulargewicht | 14-17 kDa |
GenBank-Zugangsnummer | BC000897 |
Gene symbol | IFITM1 |
Gene ID (NCBI) | 8519 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Protein-A-Reinigung |
Lagerungspuffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
IFITM1(interferon induced transmembrane protein), also named DSPA2a and interferon-induced protein 17 (IFI17), belongs to the CD225 family. It has two transmembrane domain and serves as an IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, influenza A H1N1 virus, West Nile virus (WNV), and dengue virus , by inhibiting the early steps of replication. IFITM proteins are recently identified as viral restriction factors that inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. Also they serve as important components of the innate immune system to restrict HIV-1 infection. 60074-1-Ig is a mouse monoclonal antibody which specifically recognizing IFITM1 but not IFITM2 or IFITM3.
Protokolle
Produktspezifische Protokolle | |
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WB protocol for IFITM1-Specific antibody 60074-1-Ig | Protokoll herunterladen |
IHC protocol for IFITM1-Specific antibody 60074-1-Ig | Protokoll herunterladen |
IF protocol for IFITM1-Specific antibody 60074-1-Ig | Protokoll herunterladen |
IP protocol for IFITM1-Specific antibody 60074-1-Ig | Protokoll herunterladen |
FC protocol for IFITM1-Specific antibody 60074-1-Ig | Protokoll herunterladen |
Standard-Protokolle | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Science IFITM proteins inhibit placental syncytiotrophoblast formation and promote fetal demise. | ||
Cell The IFITM proteins mediate cellular resistance to influenza A H1N1 virus, West Nile virus, and dengue virus. | ||
Sci Transl Med Dimethyl fumarate potentiates oncolytic virotherapy through NF-κB inhibition. | ||
Cell Host Microbe Resistance of Transmitted Founder HIV-1 to IFITM-Mediated Restriction.
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Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Claudia (Verified Customer) (06-20-2022) | The antibody worked very well for the analysis of IFITM1 expression in permanent cell lines.
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