IFIT2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

IFIT2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

42

Kat-Nr. : 12604-1-AP

Synonyme

IFI 54K, IFI 54, GARG 39, G10P2, CIG-42

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Galerie der Validierungsdaten

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  WB analysis of A431 cells using 12604-1-AP

  WB result of IFIT2 antibody (12604-1-AP, 1:300) with si-IFIT2 and si-Control transfected A431 cells.

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  WB analysis using 12604-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12604-1-AP (IFIT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW 264.7 using 12604-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 12604-1-AP (IFIT2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of A431 using 12604-1-AP

  IP result of anti-IFIT2 (IP:12604-1-AP, 4ug; Detection:12604-1-AP 1:2000) with A431 cells lysate 1200 ug.

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  IHC staining of human kidney using 12604-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12604-1-AP (IFIT2 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin cancer using 12604-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 12604-1-AP (IFIT2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 12604-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 12604-1-AP (IFIT2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 12604-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12604-1-AP (IFIT2 Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HepG2 using 12604-1-AP

  Immunofluorescent analysis of HepG2 cells using 12604-1-AP (IFIT2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, RAW 264.7-Zellen, THP-1-Zellen
• Erfolgreiche IP: A431-Zellen
• Erfolgreiche Detektion in IHC: humanes Hautkrebsgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 40 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Produktinformation

12604-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA IFIT2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IFIT2 fusion protein Ag3283
• Vollständiger Name: IFIT 2
• Berechnetes Molekulargewicht: 484 aa, 56 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa
• GenBank-Zugangsnummer: BC032839
• Gene symbol: IFIT2
• Gene ID (NCBI): 3433
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IFIT2 belongs to the IFIT family. It contains 6 TPR repeats. IFIT genes comprise a large family with three (Ifit1, Ifit2, and Ifit3) and four (IFIT1, IFIT2, IFIT3, and IFIT5) members in mice and humans, respectively. IFIT proteins are produced in human body that are supposed to confer immunity against viral infection. These proteins are generally produced during viral infection, IFN treatment, and during pathogen recognition (Pathogen associated molecular pattern recognition) by immune system during infections.

Publikationen

Species	Application	Title
human	WB	Signal Transduct Target Ther; TRAF3 activates STING-mediated suppression of EV-A71 and target of viral evasion; Authors - Wenwen Zheng
human	WB	Nat Immunol; A phosphomimetic-based mechanism of dengue virus to antagonize innate immunity.; Authors - Ying Kai Chan
human	WB	Mol Cell; Translation stress and collided ribosomes are co-activators of cGAS.; Authors - Li Wan
human	WB	Nat Microbiol; ISG15-dependent activation of the sensor MDA5 is antagonized by the SARS-CoV-2 papain-like protease to evade host innate immunity.; Authors - GuanQun Liu
human	WB	Nat Microbiol; Influenza virus repurposes the antiviral protein IFIT2 to promote translation of viral mRNAs.; Authors - Vy Tran
		Nucleic Acids Res; IFIT3 and IFIT2/3 promote IFIT1-mediated translation inhibition by enhancing binding to non-self RNA.; Authors - Renata C Fleith

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