CUL2 Polyklonaler Antikörper

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• KD/KO Validated

CUL2 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, IHC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Kat-Nr. : 10981-2-AP

Synonyme

CUL 2, CUL2, cullin 2

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Galerie der Validierungsdaten

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  WB analysis using 10981-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10981-2-AP (CUL2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 10981-2-AP

  WB result of CUL2 antibody (10981-2-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CUL2 transfected PC-3 cells.

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  IP experiment of HeLa using 10981-2-AP

  IP result of anti-CUL2 (IP:10981-2-AP, 4ug; Detection:10981-2-AP 1:2000) with HeLa cells lysate 1360 ug.

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  IHC staining of human prostate cancer using 10981-2-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10981-2-AP (CUL2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 10981-2-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10981-2-AP (CUL2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, PC-3-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 11 publications below
• CoIP: See 1 publications below

Produktinformation

10981-2-AP bindet in WB, IP, IHC, CoIP, ELISA CUL2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CUL2 fusion protein Ag1434
• Vollständiger Name: cullin 2
• Berechnetes Molekulargewicht: 87 kDa
• Beobachtetes Molekulargewicht: 87 kDa
• GenBank-Zugangsnummer: BC009591
• Gene symbol: CUL2
• Gene ID (NCBI): 8453
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The cullin family proteins are scaffold proteins for the Ring finger type E3 ligases, participating in the proteolysis through the ubiquitin-proteasome pathway. Humans express seven cullin proteins: CUL1-3, CUL4A, CUL4B, CUL5, and CUL7. Each cullin protein can form an E3 ligase similar to the prototype Ring-type E3 ligase Skp1-CUL1-F-box complex. The Cullin-RING-finger type E3 ligases are important regulators in early embryonic development, as highlighted by genetic studies demonstrating that knock-out of CUL1, CUL3, or CUL4A in mice results in early embryonic lethality.

Publikationen

Species	Application	Title
human	WB	Dev Cell; AMBRA1 interplay with cullin E3 ubiquitin ligases regulates autophagy dynamics.; Authors - Manuela Antonioli ; KD Validated
human	WB	Cell Death Differ; CRL2-KLHDC3 E3 ubiquitin ligase complex suppresses ferroptosis through promoting p14ARF degradation.; Authors - Pingzhao Zhang
human	WB	Oncogene; iASPP is essential for HIF-1α stabilization to promote angiogenesis and glycolysis via attenuating VHL-mediated protein degradation.; Authors - Dong Zhao
human	WB	Aging (Albany NY); Tumor suppressor DCAF15 inhibits epithelial-mesenchymal transition by targeting ZEB1 for proteasomal degradation in hepatocellular carcinoma.; Authors - Xiao Dong ; KD Validated
human	WB	Mol Cell Biol; DNA damage regulates UHRF1 stability via the SCF(β-TrCP) E3 ligase.; Authors - Chen Hao H ; KD Validated
human	WB	J Cell Sci; Cullin-3-KCTD10-mediated CEP97 degradation promotes primary cilium formation.; Authors - Tomoaki Nagai ; KD Validated

Verwandte Produkte

TCEB1 Polyclonal antibody

12450-1-AP

CUL4B Polyclonal antibody

12916-1-AP

HIF-1 alpha Polyclonal antibody

20960-1-AP

RBX1 Polyclonal antibody

14895-1-AP

COMMD1 Polyclonal antibody

11938-1-AP

CUL3 Polyclonal antibody

11107-1-AP