CUL3 Polyclonal antibody

• Featured Product
• KD/KO Validated

CUL3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

20

Reviews (1)

Cat no : 11107-1-AP

Synonyms

KIAA0617, Cullin-3, CUL-3

---

Validation Data Gallery

• 

  WB analysis using 11107-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11107-1-AP (CUL3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11107-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11107-1-AP (CUL3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 11107-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11107-1-AP (CUL3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 11107-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11107-1-AP (CUL3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 11107-1-AP

  Immunofluorescent analysis of HEK-293 cells using 11107-1-AP (CUL3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, SH-SY5Y cells, NIH/3T3 cells, Neuro cells, PC-12 cells, mouse testis tissue, rat testis tissue, mouse brain tissue
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 18 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Product Information

11107-1-AP targets CUL3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CUL3 fusion protein Ag1555
• Full Name: cullin 3
• Calculated Molecular Weight: 89 kDa
• Observed Molecular Weight: 80-89 kDa
• GenBank Accession Number: BC039598
• Gene Symbol: CUL3
• Gene ID (NCBI): 8452
• RRID: AB_2086429
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ligase are the largest family of ubiquitin ligases which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. One of seven cullin proteins serves as a scaffold protein for the assembly of the multisubunit ubiquitin ligase complex. CUL3 (Cullin-3) ubiquitin ligase complex targets multiple substrate for ubiquitination including cyclin E and of cyclin D1. Defects in CUL3 are the cause of Pseudohypoaldosteronism type 2E (PHA2E) characterized by severe hypertension, hyperkalemia, hyperchloremia, hyperchloremic metabolic acidosis, and correction of physiologic abnormalities by thiazide diuretics.

Publications

Species	Application	Title
human	WB	Adv Sci (Weinh); DDRGK1 Enhances Osteosarcoma Chemoresistance via Inhibiting KEAP1-Mediated NRF2 Ubiquitination; Authors - Xin Wang
human	WB	Free Radic Biol Med; β-Sitosterol targets ASS1 for Nrf2 ubiquitin-dependent degradation, inducing ROS-mediated apoptosis via the PTEN/PI3K/AKT signaling pathway in ovarian cancer; Authors - Haoyu Wang
human	WB	Sci Total Environ; USP15 participates in DBP-induced testicular oxidative stress injury through regulating the Keap1/Nrf2 signaling pathway.; Authors - Lei Zhang
human	WB	Food Chem; Tangeretin maintains antioxidant activity by reducing CUL3 mediated NRF2 ubiquitination.; Authors - Yue Wang
human	WB	Aging (Albany NY); Tumor suppressor DCAF15 inhibits epithelial-mesenchymal transition by targeting ZEB1 for proteasomal degradation in hepatocellular carcinoma.; Authors - Xiao Dong ; KD Validated
human	WB,IF,IP	FEBS J; CUL3 induces mitochondrial dysfunction via MRPL12 ubiquitination in renal tubular epithelial cells; Authors - Xingzhao Ji

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Brittney (Verified Customer) (11-26-2018)

10ug load of protein. Cells lysed in RIPA buffer then added to 2X Laemmli buffer for denaturing. Heated at 95deg for 10 minutes as well.Multiple bands, but all follow same pattern. Assumed the band at ~75 kD was the correct one.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HK-2 Cells

Related Products

CUL2 Polyclonal antibody

10981-2-AP

CUL1 Polyclonal antibody

12895-1-AP

CUL4A Monoclonal antibody

66038-1-Ig

KEAP1 Polyclonal antibody

10503-2-AP

KLHL3 Polyclonal antibody

16951-1-AP

NEDD8 Polyclonal antibody

16777-1-AP