COMMD1 Polyclonal antibody

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  WB analysis using 11938-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11938-1-AP (COMMD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 11938-1-AP

  IP result of anti-COMMD1 (IP:11938-1-AP, 3ug; Detection:11938-1-AP 1:500) with mouse brain tissue lysate 5200ug.

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  IHC staining of human liver cancer using 11938-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11938-1-AP (COMMD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 11938-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11938-1-AP (COMMD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 11938-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using COMMD1 antibody (11938-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

COMMD1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 11938-1-AP

Publications(24)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

Protein Murr1, MURR1, COMM domain-containing protein 1, C2orf5

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 6 publications below
• WB: See 20 publications below
• IHC: See 4 publications below
• IF: See 5 publications below
• CoIP: See 3 publications below

Product Information

11938-1-AP targets COMMD1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2535

  Product name: Recombinant human COMMD1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-190 aa of BC022046

  Sequence: MAAGELEGGKPLSGLLNALAQDTFHGYPGITEELLRSQLYPEVPPEEFRPFLAKMRGILKSIASADMDFNQLEAFLTAQTKKQGGITSDQAAVISKFWKSHKTKIRESLMNQSRWNSGLRGLSWRVDGKSQSRHSAQIHTPVAIIELELGKYGQESEFLCLEFDEVKVNQILKTLSEVEESISTLISQPN

• Full Name: copper metabolism (Murr1) domain containing 1
• Calculated Molecular Weight: 190 aa, 21 kDa
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: BC022046
• Gene Symbol: COMMD1
• Gene ID (NCBI): 150684
• RRID: AB_2083542
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8N668
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

COMMD1(COMM domain-containing protein 1), also named MURR1, is implicated in copper homeostasis by binding one copper ion per monomer. COMMD1 was reported to accelerate the ubiquitination and degradation of NF-kappa-B subunits, its deficiency exerts enhanced NF-kappa-B mediated celluar response. Ubiquitinated by XIAP (X-linked inhibitor of apoptosis), COMMD1 undergoes proteasomal degradation. SOD1 activity can be downregulated by COMMD1.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human,mouse	WB,IF	Circ Res; COMMD Family Regulates Plasma LDL Levels and Attenuates Atherosclerosis Through Stabilizing the CCC Complex in Endosomal LDLR Trafficking.; Authors - Alina Fedoseienko ; KD Validated
human	WB	Nat Commun; A genome-wide CRISPR screen identifies host factors that regulate SARS-CoV-2 entry.; Authors - Yunkai Zhu
canine	WB,IF	Nat Commun; CCC- and WASH-mediated endosomal sorting of LDLR is required for normal clearance of circulating LDL.; Authors - Paulina Bartuzi ; KO Validated
human		J Cell Biol; HRS-WASH axis governs actin-mediated endosomal recycling and cell invasion.; Authors - Ewan MacDonald
human	WB,IF,CoIP	Cell Rep; COMMD5/HCaRG Hooks Endosomes on Cytoskeleton and Coordinates EGFR Trafficking.; Authors - Carole G Campion ; KD Validated
human	WB	Theranostics; SPTBN1 inhibits inflammatory responses and hepatocarcinogenesis via the stabilization of SOCS1 and downregulation of p65 in hepatocellular carcinoma.; Authors - Ling Lin

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Emilia (Verified Customer) (11-30-2018)

works well for WB

• Applications: Western Blot,
• Primary Antibody Dilution: 1;1000
• Cell Tissue Type: 293, Cos1