CUL4B Polyclonal antibody

• Featured Product
• KD/KO Validated

CUL4B Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Conjugate

Unconjugated

Publications

80

Cat no : 12916-1-AP

Synonyms

KIAA0695, Cullin-4B, cullin 4B

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Validation Data Gallery

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  WB analysis using 12916-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12916-1-AP (CUL4B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa cells using 12916-1-AP

  WB result of CUL4B antibody (12916-1-AP, 1:2,000) with si-Control and si-CUL4B transfected HeLa cells.

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  IP experiment of HeLa using 12916-1-AP

  IP result of anti-CUL4B (IP:12916-1-AP, 3ug; Detection:12916-1-AP 1:1000) with HeLa cells lysate 4650ug.

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  IF Staining of HeLa using 12916-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CUL4B antibody (12916-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 12916-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CUL4B antibody (12916-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 12916-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CUL4B antibody (12916-1-AP) at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells
• Positive IP detected in: HeLa cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 29 publications below
• WB: See 75 publications below
• IHC: See 4 publications below
• IF: See 8 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below
• RIP: See 1 publications below

Product Information

12916-1-AP targets CUL4B in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CUL4B fusion protein Ag3563
• Full Name: cullin 4B
• Calculated Molecular Weight: 913 aa, 104 kDa
• Observed Molecular Weight: 102 kDa
• GenBank Accession Number: BC036216
• Gene Symbol: CUL4B
• Gene ID (NCBI): 8450
• RRID: AB_2086699
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cullin-RING ligases (CRLs) complexes participate in the regulation of diverse cellular processes, including cell cycle progression, transcription, signal transduction and development (PMID: 21816341)(PMID: 21554755). Serving as the scaffold protein, cullins are crucial for the assembly of ligase complexes, which recognize and target various substrates for proteosomal degradation. Two cullin 4 (CUL4) proteins, CUL4A (87 kDa) and CUL4B(104 kDa), are two members in cullin family with 83% of identity. Mutations in human CUL4B are one of the major causes of X-linked mental retardation. Cul4b knockout mice demonstrated that CUL4B is indispensable for embryonic development in the mouse (PMID: 22606329). This antibody may detect both CUL4A (87 kDa) and CUL4B(104 kDa).

Publications

Species	Application	Title
human	WB	Nature; Targeted protein degradation via intramolecular bivalent glues; Authors - Oliver Hsia ; KD Validated
human	CoIP,WB	Cell; FBXO44 promotes DNA replication-coupled repetitive element silencing in cancer cells.; Authors - Jia Z Shen ; KD Validated
human	WB	Cell; Systematic discovery of mutation-directed neo-protein-protein interactions in cancer.; Authors - Xiulei Mo
human	IHC,IF	Science; CRL4 complex regulates mammalian oocyte survival and reprogramming by activation of TET proteins.; Authors - Chao Yu
human	WB	Nat Med; Immunomodulatory drugs disrupt the cereblon-CD147-MCT1 axis to exert antitumor activity and teratogenicity.; Authors - Ruth Eichner ; KD Validated
human	WB	Science; Anticancer sulfonamides target splicing by inducing RBM39 degradation via recruitment to DCAF15.; Authors - Ting Han

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