RBX1 Polyclonal antibody

RBX1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

14

Cat no : 14895-1-AP

Synonyms

Protein ZYP, RBX1, Regulator of cullins 1, ring box 1, RING box protein 1, RING finger protein 75, RNF75, ROC1

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Validation Data Gallery

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  WB analysis using 14895-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14895-1-AP (RBX1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14895-1-AP

  IP result of anti-RBX1 (IP:14895-1-AP, 4ug; Detection:14895-1-AP 1:500) with HeLa cells lysate 1520ug.

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  IHC staining of human heart using 14895-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14895-1-AP (RBX1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 14895-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14895-1-AP (RBX1 antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 14 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

14895-1-AP targets RBX1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat, eriocheir sinensis
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RBX1 fusion protein Ag6685
• Full Name: ring-box 1
• Calculated Molecular Weight: 12 kDa
• Observed Molecular Weight: 15 kDa
• GenBank Accession Number: BC001466
• Gene Symbol: RBX1
• Gene ID (NCBI): 9978
• RRID: AB_2179719
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RBX1(RING-box protein 1) is also named as RNF75, ROC1 and is a requisite component of the multi- subunit SCF family E3s36-40.It mediates the ubiquitination and subsequent proteasomal degradation of target proteins, including proteins involved in cell cycle progression, signal transduction, transcription and transcription-coupled nucleotide excision repair.

Publications

Species	Application	Title
human	WB	Cell Death Differ; CRL2-KLHDC3 E3 ubiquitin ligase complex suppresses ferroptosis through promoting p14ARF degradation.; Authors - Pingzhao Zhang
human	WB	Elife; Discovery of a molecular glue promoting CDK12-DDB1 interaction to trigger cyclin K degradation.; Authors - Lu Lv
human	WB	Cell Death Dis; Bcl-3 regulates TGFβ signaling by stabilizing Smad3 during breast cancer pulmonary metastasis.; Authors - Xi Chen
human,mouse,rat	WB	Neurobiol Aging; SQSTM1/ p62 oligomerization contributes to Aβ-induced inhibition of Nrf2 signaling.; Authors - Chenjia Guo
human	WB,IP	Microvasc Res; A positive feedback loop between miR-574-3p and HIF-1α in promoting angiogenesis under hypoxia; Authors - Renwen Zhang
Eriocheir sinensis	WB,IF	Gene; Expression pattern and functional analysis of the two RING box protein RBX in spermatogenesis of Chinese mitten crab Eriocheir sinensis.; Authors - Jia-Shun Zhang