BAX Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

BAX Polyklonal Antikörper für FC, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (6)

Anwendung

WB, IP, IHC, FC, CoIP, chIP, ELISA

Konjugation

Unkonjugiert

Publikationen

2848

Rezensionen (4)

Kat-Nr. : 50599-2-Ig

Synonyme

Apoptosis regulator BAX, BAX

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Galerie der Validierungsdaten

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  WB analysis using 50599-2-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 50599-2-Ig (BAX antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 50599-2-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 50599-2-Ig (BAX antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 50599-2-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 50599-2-Ig (BAX antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. Jurkat cell lysate used as a negative control.

• 

  WB analysis of HeLa cells using 50599-2-Ig

  WB result of BAX antibody (50599-2-Ig, 1:5000) with si-Control and si-BAX transfected HeLa cells.

• 

  WB analysis using 50599-2-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 50599-2-Ig (BAX antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 50599-2-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 50599-2-Ig (BAX antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 50599-2-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 50599-2-Ig (BAX antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 50599-2-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 50599-2-Ig (BAX antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 50599-2-Ig

  Various lysate were subjected to SDS PAGE followed by western blot with 50599-2-Ig (BAX antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 50599-2-Ig

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 50599-2-Ig (BAX antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 50599-2-Ig

  PC-12 cells were subjected to SDS PAGE followed by western blot with 50599-2-Ig (BAX antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Raji using 50599-2-Ig

  IP result of anti-BAX (IP:50599-2-Ig, 4ug; Detection:50599-2-Ig 1:1000) with Raji cells lysate 3160 ug.

• 

  IP experiment of mouse heart using 50599-2-Ig

  IP result of anti-BAX(IP:50599-2-Ig, 4ug; Detection:50599-2-Ig 1:6000) with mouse heart tissue lysate 1680 ug.

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  IHC staining of mouse lung using 50599-2-Ig

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 50599-2-Ig (BAX antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 50599-2-Ig

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 50599-2-Ig (BAX antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 50599-2-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 50599-2-Ig (BAX antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 50599-2-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 50599-2-Ig (BAX antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of Ramos using 50599-2-Ig

  1X10^6 Ramos cells were intracellularly stained with 0.4 ug Anti-Human BAX (50599-2-Ig) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, COLO 320-Zellen, HeLa-Zellen, HT-1080.Zellen, Mausherzgewebe, Mausnierengewebe, NIH/3T3-Zellen, PC-12-Zellen, Raji-Zellen, Rattenhirngewebe, Ratten-Kolongewebe, Rattenlungengewebe, Rattennierengewebe, RAW 264.7-Zellen
• Erfolgreiche IP: Raji-Zellen, Mausherzgewebe
• Erfolgreiche Detektion in IHC: Mauslungengewebe, Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: Ramos-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 9 publications below
• WB: See 2747 publications below
• IHC: See 157 publications below
• IP: See 5 publications below
• ELISA: See 1 publications below
• FC: See 1 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Produktinformation

50599-2-Ig bindet in WB, IP, IHC, FC, CoIP, chIP, ELISA BAX und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, hamster, Hausschwein, Huhn, Kaninchen, Maus, Ratte, Zebrafisch, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BAX fusion protein Ag0576
• Vollständiger Name: BCL2-associated X protein
• Berechnetes Molekulargewicht: 21 kDa
• Beobachtetes Molekulargewicht: 21 kDa
• GenBank-Zugangsnummer: BC014175
• Gene symbol: BAX
• Gene ID (NCBI): 581
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BAX (also known as BCL2 Associated X, Bcl-2-Like Protein 4, Bcl2-L-4, BCL2L4) is a member of the BCL2 family of proteins that play a key role in the regulation of apoptosis in higher eukaryotes (https://www.uniprot.org/uniprot/Q07812). BAX comprises 4 Bcl-2 homology domains (BH1-BH4) and a C-terminal transmembrane domain. In healthy mammalian cells, BAX is localized to the cytoplasm through its interaction with the anti-apoptotic BL-2 family members BCL2L1/Bcl-xL (PMIDs: 28755482, 21458670). In response to apoptotic stimuli, however, BAX undergoes a conformational change that causes it to translocate to the outer mitochondrial membrane where it initiates the mitochondrial pathway of apoptosis via two potential mechanisms. Firstly, upon translocation to the outer mitochondrial membrane, BAX interacts with the mitochondrial voltage-dependent anion channel (VDAC) leading to the opening of the channel, loss of membrane potential, and the release of cytochrome c from the mitochondrion (PMID:10766872). The release of cytochrome C into the cytoplasm leads to the activation of Caspase3, initiating apoptosis. Secondly, activated BAX forms homodimers, which then assemble into oligomers on the mitochondrial outer membrane to create pores that permeabilize the mitochondrion leading to the release of cytochrome C (PMID:25458844).

BAX has been shown to be involved in p53-mediated apoptosis. Expression of the human bax gene has been shown to be directly regulated by p53, and the bax promoter contains four motifs with homology to consensus p53-binding sites (PMID:7834749). Furthermore, p53 directly interacts with BAX to promote its activation (PMID:14963330).

What is the molecular weight of BAX?

BAX is a 192 amino acid protein that has a predicted molecular weight of 21.1 kDa.

What is the subcellular localization of BAX?

In healthy mammalian cells, BAX is localized to the cytoplasm. In response to apoptotic stimuli, BAX translocates to the outer mitochondrial membrane.

 What is the tissue specificity of BAX?

BAX is ubiquitously expressed (PMID: 25613900).

What is the connection between BAX and cancer?

BAX appears to play an important role in suppressing cancer development, and decreased BAX levels are associated with chemo- and radioresistance in a number of cancers including lung cancer, chronic lymphocytic leukemia (CLL), and prostate cancer. Loss-of-function mutations of BAX have also been reported in hematopoietic malignancies in humans (PMID:9531611) and in gastrointestinal cancer of the microsatellite mutator phenotype (MMP), where BAX inactivation contributes to tumor progression by providing a survival advantage (PMID:9331106).

A number of anticancer drugs used clinically have been demonstrated to induce BAX activation indirectly to facilitate apoptosis of tumor cells. Recent efforts have demonstrated that BAX itself may be a promising direct target for small-molecule drug discovery for novel anticancer drugs and several direct BAX activators have been identified that may hold promise for cancer therapy, with the potential to overcome chemo- and radioresistance (PMID:25230299).

Publikationen

Species	Application	Title
human	WB	Cell; The Cytoplasmic DNA Sensor cGAS Promotes Mitotic Cell Death.; Authors - Christian Zierhut
mouse	WB	Bioact Mater; Chemo-immunotherapy by dual-enzyme responsive peptide self-assembling abolish melanoma; Authors - Yuhan Wang
mouse	WB	ACS Nano; Nanointegrative In Situ Reprogramming of Tumor-Intrinsic Lipid Droplet Biogenesis for Low-Dose Radiation-Activated Ferroptosis Immunotherapy; Authors - Qiqi Zhang
human	WB	Mol Cell; Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.; Authors - Bin Jiang ; KO Validated
human	WB	Cell Res; Tom20 senses iron-activated ROS signaling to promote melanoma cell pyroptosis.; Authors - Bo Zhou ; KD Validated
human	WB	Acta Pharm Sin B; Miltirone induces cell death in hepatocellular carcinoma cell through GSDME-dependent pyroptosis.; Authors - Xiaowei Zhang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Macarena Lucia (Verified Customer) (10-17-2022)

nice band

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000

FH

ZEE (Verified Customer) (07-13-2020)

VERY GOOD antibody when western

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: MOUSE HEART

FH

Tanusree (Verified Customer) (12-18-2019)

Product worked well in WB at 1:500 dilution

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse brain

FH

Kathryn (Verified Customer) (11-04-2019)

Image shows a post-natal day 2 mouse ovary with germ cells marked by TRA98 (Abcam) in green, and BAX (Proteintech) marked in red.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: mouse ovary

BAX Antibody Immunofluorescence, validation (1:100 dilution) in mouse ovary (Cat no:50599-2-Ig)

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