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SURVIVIN Polyclonal antibody
SURVIVIN Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (3)
Applications
WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA
Conjugate
Unconjugated
150
Cat no : 10508-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, PC-3 cells, Jurkat cells, NIH/3T3 cells, A549 cells, MCF-7 cells, A431 cells, Ramos cells |
| Positive IP detected in | U2OS cells, Jurkat cells |
| Positive IHC detected in | human colon cancer tissue, human urothelial carcinoma tissue, human stomach cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
| Positive FC (Intra) detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 135 publications below |
| IHC | See 2 publications below |
| CoIP | See 1 publications below |
Product Information
10508-1-AP targets SURVIVIN in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, canine, hamster |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SURVIVIN fusion protein Ag0788 |
| Full Name | baculoviral IAP repeat-containing 5 |
| Calculated Molecular Weight | 16 kDa |
| Observed Molecular Weight | 16-18 kDa |
| GenBank Accession Number | BC008718 |
| Gene Symbol | Survivin |
| Gene ID (NCBI) | 332 |
| RRID | AB_2064048 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Survivin, also called BIRC5, is a unique member of the inhibitor of apoptosis (IAP) protein family. Survivin is a 16 kDa anti-apoptotic protein highly expressed during fetal development and cancer cell malignancy, but is completely absent in terminally differentiated cells. The differential expression of survivin in cancer versus normal tissues makes it a useful tool in cancer diagnosis and a promising therapeutic target. Survivin expression is also highly regulated by the cell cycle and is only expressed in the G2-M phase. It is known that survivin localizes to the mitotic spindle by interaction with tubulin during mitosis and may play a contributing role in regulating mitosis. Disruption of survivin-microtubule interactions results in loss of survivin's anti-apoptosis function and increased caspase-3 activity, a mechanism involved in cell death, during mitosis. It also is a direct target gene of the Wnt pathway and is upregulated by beta-catenin.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SURVIVIN antibody 10508-1-AP | Download protocol |
| IHC protocol for SURVIVIN antibody 10508-1-AP | Download protocol |
| IF protocol for SURVIVIN antibody 10508-1-AP | Download protocol |
| IP protocol for SURVIVIN antibody 10508-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Theranostics Histone H3K27 methyltransferase EZH2 regulates apoptotic and inflammatory responses in sepsis-induced AKI | ||
Autophagy Elaiophylin, a novel autophagy inhibitor, exerts antitumor activity as a single agent in ovarian cancer cells. | ||
Circ Res Iron Homeostasis and Pulmonary Hypertension: Iron Deficiency Leads to Pulmonary Vascular Remodeling in the Rat. | ||
J Exp Clin Cancer Res ACTN1 promotes HNSCC tumorigenesis and cisplatin resistance by enhancing MYH9-dependent degradation of GSK-3β and integrin β1-mediated phosphorylation of FAK | ||
Cancer Lett SKP2 promotes the metastasis of pancreatic ductal adenocarcinoma by suppressing TRIM21-mediated PSPC1 degradation |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Susmita (Verified Customer) (08-21-2020) | Giving good result and crisp band in Western Blot.
 |
FH Iram (Verified Customer) (08-14-2020) | We got very sharp and clean band of survivin with this antibody.
|
