XIAP Polyclonal antibody

• 

  WB analysis using 10037-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 10037-1-Ig (XIAP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10037-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10037-1-Ig (XIAP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 10037-1-Ig

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 10037-1-Ig (XIAP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10037-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 10037-1-Ig (XIAP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10037-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10037-1-Ig (XIAP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 10037-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10037-1-Ig(XIAP antibody) at dilution of 1:200 (under 40x lens).

• Featured Product
• KD/KO Validated

XIAP Polyclonal Antibody for WB, IHC, ELISA

Cat No. 10037-1-Ig

Publications(51)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, CoIP, ELISA

Synonyms

API3, Baculoviral IAP repeat-containing protein 4, E3 ubiquitin-protein ligase XIAP, EC:2.3.2.27, hIAP-3

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Tested Applications

• Positive WB detected in: RAW 264.7 cells, Jurkat cells, HepG2 cells, COLO 320 cells, HeLa cells, C6 cells, NIH/3T3 cells, Raji cells
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 45 publications below
• IHC: See 11 publications below
• CoIP: See 1 publications below

Product Information

10037-1-Ig targets XIAP in WB, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag0022

  Product name: Recombinant human XIAP protein

  Source: e coli.-derived, Unknow

  Tag: N/A

  Domain: XIAP of BC032729

  Sequence:

• Full Name: X-linked inhibitor of apoptosis
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 45-57 kDa
• GenBank Accession Number: BC032729
• Gene Symbol: XIAP
• Gene ID (NCBI): 331
• RRID: AB_2215009
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P98170
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

XIAP, also named as API3, BIRC4 and IAP3, belongs to the IAP family. It has E3 ubiquitin-protein ligase activity. It mediates the proteasomal degradation of target proteins, such as caspase-3, SMAC or AIFM1. XIAP is an inhibitor of caspase-3, -7 and -9. It mediates activation of MAP3K7/TAK1, leading to the activation of NF-kappa-B. XIAP is an apoptotic suppressor. It is ubiquitinated and degraded by the proteasome in apoptotic cells. The MW of XIAP is 45-56 kDa.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nat Commun; Targeting dual-specificity tyrosine phosphorylation-regulated kinase 2 with a highly selective inhibitor for the treatment of prostate cancer.; Authors - Kai Yuan
human	WB	Cancer Res; DAB2IP is a bifunctional tumor suppressor that regulates wildtype RAS and inflammatory cascades in KRAS mutant colon cancer; Authors - Abigail L Miller
human	IHC	Cancer Res; An ATM/TRIM37/NEMO axis counteracts genotoxicity by activating nuclear-to-cytoplasmic NF-κB signaling.; Authors - Geyan Wu
human	WB	EMBO Rep; XIAP-mediated degradation of IFT88 disrupts HSC cilia to stimulate HSC activation and liver fibrosis; Authors - Renjie Hong ; KD Validated
human	WB	Acta Pharmacol Sin; Aspirin modulates succinylation of PGAM1K99 to restrict the glycolysis through NF-κB/HAT1/PGAM1 signaling in liver cancer.; Authors - Yu-Fei Wang
human	WB	Int J Nanomedicine; Synergism of cisplatin-oleanolic acid co-loaded calcium carbonate nanoparticles on hepatocellular carcinoma cells for enhanced apoptosis and reduced hepatotoxicity.; Authors - Muhammad Waseem Khan