Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 80001-1-RR (TDP43 recombinant antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-Rabbit Recombinant secondary antibody RGAR011 was used for detection.

Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 80008-1-RR (smooth muscle actin recombinant antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection.

Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 80008-1-RR (smooth muscle actin recombinant antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection

Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 12715-1-AP (Dystrophin polyclonal antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection.

Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 20874-1-AP (E-cadherin polyclonal antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection.

Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 20874-1-AP (E-cadherin polyclonal antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection.

Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 80165-1-RR (TMEM173/STING antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection.

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using commercial IVD grade KI67 antibody. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection.

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using other vendor's NF-kappa B p65 rabbit recombinant antibody. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection.

Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using other vendor's BCL2 rabbit recombinant antibody. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection.

Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 12255-1-AP (RCAS1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection. The staining was performed on Celnovte's CNT360-M1 autostainer.

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 12255-1-AP (RCAS1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection. The staining was performed on Celnovte's CNT360-M1 autostainer.

Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 12715-1-AP (Dystrophin antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection. The staining was performed on Celnovte's CNT360-M1 autostainer. Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection. The staining was performed on Celnovte's CNT360-M1 autostainer.

Immunohistochemical analysis of paraffin-embedded mouse tongue tissue slide using 12715-1-AP (Dystrophin antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection. The staining was performed on Celnovte's CNT360-M1 autostainer.

Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 12715-1-AP (Dystrophin antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection. The staining was performed on Celnovte's CNT360-M1 autostainer.

Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 20874-1-AP (E-cadherin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection. The staining was performed on Celnovte's CNT360-M1 autostainer.

Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20874-1-AP (E-cadherin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection. The staining was performed on Celnovte's CNT360-M1 autostainer.

Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 80001-1-RR (TDP-43 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection. The staining was performed on Celnovte's CNT360-M1 autostainer.

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 80001-1-RR (TDP-43 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection. The staining was performed on Celnovte's CNT360-M1 autostainer.

Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 80001-1-RR (TDP-43 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection. The staining was performed on Celnovte's CNT360-M1 autostainer.

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 81668-1-RR (F4/80 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection. The staining was performed on Celnovte's CNT360-M1 autostainer.

Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 81668-1-RR (F4/80 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection. The staining was performed on Celnovte's CNT360-M1 autostainer.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using other vendor's ER rabbit recombinant antibody. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection.

Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 21146-1-AP (LARS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection.

Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 21146-1-AP (LARS antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection.

Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66096-1-Ig (Villin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-Mouse Recombinant secondary antibody RGAM011 was used for detection.

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66065-2-Ig (CD31 mouse monoclonal antibody) at dilution of 1:10000 . Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-mouse Recombinant secondary antibody RGAM011 was used for detection.

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66379-1-Ig (Beta Catenin mouse monoclonal antibody) at dilution of 1:20000 . Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-mouse Recombinant secondary antibody RGAM011 was used for detection.

Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using commercial IVD grade pan-CK mouse monoclonal antibody. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-mouse Recombinant secondary antibody RGAM011 was used for detection.

Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using other vendor's NeuN mouse monoclonal antibody. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-mouse Recombinant secondary antibody RGAM011 was used for detection.

Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 67479-1-Ig (SLC22A7 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-Mouse Recombinant secondary antibody RGAM011 was used for detection. The staining was performed on Celnovte's CNT360-M1 autostainer.

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66836-1-Ig (NeuN antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-Mouse Recombinant secondary antibody RGAM011 was used for detection. The staining was performed on Celnovte's CNT360-M1 autostainer.

Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66177-1-Ig (MPO antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-Mouse Recombinant secondary antibody RGAM011 was used for detection. The staining was performed on Celnovte's CNT360-M1 autostainer.

Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using commercial IVD grade pan-CK mouse monoclonal antibody. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat Anti-Rabbit/Mouse Universal Recombinant Secondary Antibody (H+L) RGAU011 was used for detection.

Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using other vendor's BCL2 rabbit recombinant antibody. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat Anti-Rabbit/Mouse Universal Recombinant Secondary Antibody (H+L) RGAU011 was used for detection.

Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 80232-1-RR (Vimentin rabbit recombinant antibody) at dilution of 1:2500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-Rabbit/Mouse Universal Recombinant Secondary Antibody RGAU011 was used for detection. The staining was performed on Celnovte's CNT360-M1 autostainer.

Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66379-1-Ig (Beta catennin mouse monoclonal recombinant antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-Rabbit/Mouse Universal Recombinant Secondary Antibody RGAU011 was used for detection. The staining was performed on Celnovte's CNT360-M1 autostainer.

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 80001-1-RR (TDP-43 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat Anti-Rabbit/Mouse Universal Recombinant secondary antibody RGAU011 was used for detection.

Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 12715-1-AP (Dystrophin antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat Anti-Rabbit/Mouse Universal Recombinant secondary antibody RGAU011 was used for detection.

Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 81668-1-RR (F4/80 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat Anti-Rabbit/Mouse Universal Recombinant secondary antibody RGAU011 was used for detection.

Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 66379-1-Ig (Beta Catenin antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat Anti-Rabbit/Mouse Universal Recombinant secondary antibody RGAU011 was used for detection.

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66836-1-Ig (NeuN antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat Anti-Rabbit/Mouse Universal Recombinant secondary antibody RGAU011 was used for detection.

Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66177-1-Ig (MPO antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat Anti-Rabbit/Mouse Universal Recombinant secondary antibody RGAU011 was used for detection.

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66786-1-Ig (SOX10 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat Anti-Rabbit/Mouse Universal Recombinant secondary antibody RGAU011 was used for detection.

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67300-1-Ig (CD107a / LAMP1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat Anti-Rabbit/Mouse Universal Recombinant secondary antibody RGAU011 was used for detection.

Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 67479-1-Ig (SLC22A7 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat Anti-Rabbit/Mouse Universal Recombinant secondary antibody RGAU011 was used for detection.

Various lysates were subjected to SDS-PAGE followed by western blot with rabbit anti-PSMB1 polyclonal antibody (11749-1-AP) at dilution of 1:10000. Multi-rAb HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR001) was used at 1:20000 for detection.

Various lysates were subjected to SDS-PAGE followed by western blot with rabbit anti-Beta Catenin polyclonal antibody (51067-2-AP) at dilution of 1:50000. Multi-rAb HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR001) was used at 1:20000 for detection.

Various lysates were subjected to SDS-PAGE followed by western blot with rabbit anti-TOM70 polyclonal antibody (14528-1-AP) at dilution of 1:10000. Multi-rAb HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR001) was used at 1:20000 for detection.

Various lysates were subjected to SDS-PAGE followed by western blot with rabbit anti-TDP43 recombinant antibody (80001-1-RR) at dilution of 1:20000. Multi-rAb HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR001) was used at 1:20000 for detection.

Rabbit total IgG, Mouse total IgG, Rat total IgG, Pig total IgG, Human total IgG, Bovine total IgG were coated at 100 ng/well. 0.125 μg/mL Multi-rAb HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR001) was used for detection. The result indicates that RGAM001 is highly specific for rabbit IgG and does not react with other species tested in the experiment.

Various lysates were subjected to SDS-PAGE followed by western blot with U2AF2 mouse monoclonal antibody (68166-1-Ig) at 1:20000. Multi-rAb HRP-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) RGAM001 were used at 1:20000 for detection.

Various lysates were subjected to SDS-PAGE followed by western blot with EIF3E mouse monoclonal antibody (67095-1-Ig) at dilution of 1:50000. Three batches of Multi-rAb HRP-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM001) were used at 1:20000 for detection.

Various lysates were subjected to SDS-PAGE followed by western blot with EFTUD2 mouse monoclonal antibody (67855-1-Ig, isotype IgG1) at dilution of 1:10000. RGAM001 (left) and competitor’s non cross-adsorbed HRP-Goat anti-mouse (H+L) secondary antibody (right) were both used at 0.05μg/mL for detection.

Various lysates were subjected to SDS-PAGE followed by western blot with Flag tag mouse monoclonal antibody (sigma M2, isotype IgG1) at dilution of 1:50000. RGAM001 (left) and competitor’s non cross-adsorbed HRP-Goat anti-mouse (H+L) secondary antibody (right) were both used at 0.05μg/mL for detection.

Various lysates were subjected to SDS-PAGE followed by western blot with Beta actin mouse monoclonal antibody (60008-1-Ig, isotype IgM) at dilution of 1:50000. RGAM001 (left) and competitor’s non cross-adsorbed HRP-Goat anti-mouse (H+L) secondary antibody (right) were both used at 0.05μg/mL for detection.

Mouse total IgG, Mouse IgG1, IgG2a, IgG2b, IgG2c, IgG3, IgM, IgA monoclonal antibodies, Rat total IgG, Pig total IgG, Human total IgG, Bovine total IgG were coated at 100 ng/well. 0.125 μg/mL Multi-rAb HRP-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM001) was used for detection. The result indicates that RGAM001 strongly binds to all Mouse IgGs, Mouse IgM and IgA as well as Rat IgG. It shows weak reactivity for pig IgG and does not react with other species tested in the experiment. 

Immunofluorescence of mouse cerebellum: mouse cerebellum FFPE section was stained with Rabbit anti-NeuN polyclonal antibody (26975-1-AP, 1:200, green) and mouse anti-Calbindin-D28k monoclonal antibody (66394-1-Ig, 1:200, red). Multi-rAb CoraLite® Plus 488 conjugated Recombinant Goat anti-rabbit secondary antibody (RGAR002, 1:500) and Multi-rAb CoraLite® Plus 594 conjugated Goat Anti-Mouse Recombinant Secondary Antibody (H+L) were used for detection (RGAM004, 1:500) .

Immunofluorescence of rat brain: rat brain FFPE section was stained with Rabbit anti-GFAP polyclonal antibody (16825-1-AP, 1:200, green) and mouse anti-NeuN monoclonal antibody (66836-1-Ig, orange). Multi-rAb CoraLite® Plus 488 conjugated Recombinant Goat anti-rabbit secondary antibody (RGAR002, 1:500) and Multi-rAb CoraLite® Plus 555 conjugated Goat Anti-Mouse Recombinant Secondary Antibody (H+L) were used for detection (RGAM003, 1:500) .

Immunofluorescent analysis of (4% PFA) fixed OCT-embedded frozen mouse small intestine tissue using smooth muscle actin antibody (14395-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

Immunofluorescent analysis of (4% PFA) fixed OCT-embedded frozen mouse colon tissue using E-cadherin antibody (20874-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using Myosin Light Chain 2/MLC-2V antibody (10906-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Icam-1 antibody (30324-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

Immunofluorescence of MCF-7 cells: MCF-7 cells were fixed with 4% PFA and stained with Rabbit anti-ZO1 polyclonal antibody (21773-1-AP, 1:2000, green) and mouse anti-Alpha Tubulin monoclonal antibody (66031-1-Ig, 1:1000, red). Multi-rAb CoraLite® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002, 1:500) and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004, 1:500) were used for detection.

Immunofluorescence of MCF-7 cells: MCF-7 cells were fixed with 4% PFA and stained with Rabbit anti-ZO1 polyclonal antibody (21773-1-AP, 1:2000, green) and mouse anti-Alpha Tubulin monoclonal antibody (66031-1-Ig, 1:1000, magenta). Multi-rAb CoraLite® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002, 1:500) and Multi-rAb CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM005, 1:500) were used for detection.

Immunofluorescence of Hela cells: Hela cells were fixed with 4% PFA and stained with Rabbit anti-Alpha Tubulin polyclonal antibody (11224-1-AP, 1:200, green) and mouse anti-NPM1 monoclonal antibody (60096-1-Ig, 1:1000, orange). Multi-rAb CoraLite® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002, 1:500) and Multi-rAb CoraLite® Plus 555-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM003, 1:500) were used for detection.

1X10^6 MOLT4 were surface stained with 0.2 ug Anti-HLA class I rabbit polyclonal antibody (15240-1-AP) and Rabbit IgG Isotype Control 30000-0-AP. Multi-rAb CoraLite® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) RGAR002 was used at 1:500 for detection.

Immunofluorescence of MCF-7 cells: MCF-7 cells were fixed with 4% PFA and stained with Rabbit anti-ZO1 polyclonal antibody (21773-1-AP, 1:2000, orange) and mouse anti-Alpha Tubulin monoclonal antibody (66031-1-Ig, 1:1000, green). Multi-rAb CoraLite® Plus 555-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR003, 1:500) and Multi-rAb CoraLite® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002, 1:500) were used for detection

Immunofluorescence of Hela cells: Hela cells were fixed with 4% PFA and stained with Rabbit anti-Alpha Tubulin polyclonal antibody (11224-1-AP, 1:200, orange) and mouse anti-NPM1 monoclonal antibody (60096-1-Ig, 1:1000, green). Multi-rAb CoraLite® Plus 555-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR003, 1:500) and Multi-rAbCoraLite® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002, 1:500) were used for detection.

Immunofluorescence of Hela cells: Hela cells were fixed with 4% PFA and stained with Rabbit anti-Alpha Tubulin polyclonal antibody (11224-1-AP, 1:200, red) and mouse anti-NPM1 monoclonal antibody (60096-1-Ig, 1:1000, green). Multi-rAb CoraLite® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004, 1:500) and Multi-rAb CoraLite® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002, 1:500) were used for detection.

Immunofluorescence of rat brain: rat brain FFPE section was stained with Rabbit anti-GFAP polyclonal antibody (16825-1-AP, 1:200, orange) and mouse anti-NeuN monoclonal antibody (66836-1-Ig, green). Multi-rAb CoraLite® Plus 555 conjugated Recombinant Goat anti-rabbit secondary antibody (RGAR003, 1:500) and Multi-rAb CoraLite® Plus 488 conjugated Goat Anti-Mouse Recombinant Secondary Antibody (H+L) were used for detection (RGAM002, 1:500) .

Immunofluorescence of rat brain: rat brain FFPE section was stained with Rabbit anti-GFAP polyclonal antibody (16825-1-AP, 1:200, red) and mouse anti-NeuN monoclonal antibody (66836-1-Ig, green). Multi-rAb CoraLite® Plus 594 conjugated Recombinant Goat anti-rabbit secondary antibody (RGAR004, 1:500) and Multi-rAb CoraLite® Plus 488 conjugated Goat Anti-Mouse Recombinant Secondary Antibody (H+L) were used for detection (RGAM002, 1:500) .

Immunofluorescent analysis of (4% PFA) fixed OCT-embedded frozen mouse heart tissue using ACTC1-specific antibody (66125-1-Ig, Clone: 1F2B9 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using KAP1 antibody (66630-1-Ig, Clone: 1B9G12 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-phalloidin (red).

1X10^6 MOLT4 were surface stained with 0.2 ug Anti-Human CD8 (65204-1-Ig, Clone: UCHT4) and Mouse IgG2a Isotype Control 66360-2-Ig. Multi-rAb CoraLite® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) RGAM002 was used at 1:500 for detection.

Immunofluorescence of Hela cells: Hela cells were fixed with 4% PFA and stained with Rabbit anti-Alpha Tubulin polyclonal antibody (11224-1-AP, 1:200, red) and mouse anti-NPM1 monoclonal antibody (60096-1-Ig, 1:1000, green). Multi-rAb CoraLite® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004, 1:500) and Multi-rAb CoraLite® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002, 1:500) were used for detection.

Immunofluorescent analysis of (4% PFA) fixed OCT-embedded frozen mouse colon tissue using E-cadherin antibody (20874-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004).

Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using Band 3 / AE1 antibody (81066-1-RR, Clone: 3K21 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescence of mouse cerebellum: mouse cerebellum FFPE section was stained with Rabbit anti-NeuN polyclonal antibody (26975-1-AP, 1:200, red) and mouse anti-Calbindin-D28k monoclonal antibody (66394-1-Ig, 1:200, green). Multi-rAb CoraLite® Plus 594 conjugated Recombinant Goat anti-rabbit secondary antibody (RGAR004, 1:500) and Multi-rAb CoraLite® Plus 488 conjugated Goat Anti-Mouse Recombinant Secondary Antibody (H+L) were used for detection (RGAM002, 1:500) .

Immunofluorescence of rat brain: rat brain FFPE section was stained with Rabbit anti-GFAP polyclonal antibody (16825-1-AP, 1:200, red) and mouse anti-NeuN monoclonal antibody (66836-1-Ig, green). Multi-rAb CoraLite® Plus 594 conjugated Recombinant Goat anti-rabbit secondary antibody (RGAR004, 1:500) and Multi-rAb CoraLite® Plus 488 conjugated Goat Anti-Mouse Recombinant Secondary Antibody (H+L) were used for detection (RGAM002, 1:500) .

Immunofluorescent analysis of (4% PFA) fixed OCT-embedded frozen mouse small intestine tissue using smooth muscle actin antibody (14395-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004).

Immunofluorescence of MCF-7 cells: MCF-7 cells were fixed with 4% PFA and stained with Rabbit anti-ZO1 polyclonal antibody (21773-1-AP, 1:2000, red) and mouse anti-Alpha Tubulin monoclonal antibody (66031-1-Ig, 1:1000, green). Multi-rAb CoraLite® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004, 1:500) and Multi-rAb CoraLite® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002, 1:500) were used for detection.

1X10^6 MOLT4 were surface stained with 0.2 ug Anti-HLA class I rabbit polyclonal antibody (15240-1-AP) and Rabbit IgG Isotype Control 30000-0-AP. Multi-rAb CoraLite® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) RGAR004 was used at 1:500 for detection.

Immunofluorescent analysis of (4% PFA) fixed OCT-embedded frozen mouse heart tissue using ACTC1-specific antibody (66125-1-Ig, Clone: 1F2B9 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004).

Immunofluorescence of rat brain: rat brain FFPE section was stained with Rabbit anti-GFAP polyclonal antibody (16825-1-AP, 1:200, green) and mouse anti-NeuN monoclonal antibody (66836-1-Ig, red). Multi-rAb CoraLite® Plus 488 conjugated Recombinant Goat anti-rabbit secondary antibody (RGAR002, 1:500) and Multi-rAb CoraLite® Plus 594 conjugated Goat Anti-Mouse Recombinant Secondary Antibody (H+L) were used for detection (RGAM004, 1:500) .

Immunofluorescence of MCF-7 cells: MCF-7 cells were fixed with 4% PFA and stained with Rabbit anti-ZO1 polyclonal antibody (21773-1-AP, 1:2000, green) and mouse anti-Alpha Tubulin monoclonal antibody (66031-1-Ig, 1:1000, red). Multi-rAb CoraLite® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002, 1:500) and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004, 1:500) were used for detection.

Immunofluorescence of Hela cells: Hela cells were fixed with 4% PFA and stained with Rabbit anti-Alpha Tubulin polyclonal antibody (11224-1-AP, 1:200, green) and mouse anti-NPM1 monoclonal antibody (60096-1-Ig, 1:1000, red). Multi-rAb CoraLite® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002, 1:500) and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004, 1:500) were used for detection.

Immunofluorescence of mouse cerebellum: mouse cerebellum FFPE section was stained with Rabbit anti-NeuN polyclonal antibody (26975-1-AP, 1:200, green) and mouse anti-Calbindin-D28k monoclonal antibody (66394-1-Ig, 1:200, red). Multi-rAb CoraLite® Plus 488 conjugated Recombinant Goat anti-rabbit secondary antibody (RGAR002, 1:500) and Multi-rAb CoraLite® Plus 594 conjugated Goat Anti-Mouse Recombinant Secondary Antibody (H+L) were used for detection (RGAM004, 1:500) .

1X10^6 MOLT4 were surface stained with 0.2 ug Anti-Human CD8 (65204-1-Ig, Clone: UCHT4) and Mouse IgG2a Isotype Control 66360-2-Ig. Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) RGAM004 was used at 1:500 for detection.

Immunofluorescence of Hela cells: Hela cells were fixed with 4% PFA and stained with Rabbit anti-Alpha Tubulin polyclonal antibody (11224-1-AP, 1:200, orange) and mouse anti-NPM1 monoclonal antibody (60096-1-Ig, 1:1000, green). Multi-rAb CoraLite® Plus 555-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR003, 1:500) and Multi-rAb CoraLite® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002, 1:500) were used for detection.

Immunofluorescence of mouse cerebellum: mouse cerebellum FFPE section was stained with Rabbit anti-NeuN polyclonal antibody (26975-1-AP, 1:200, orange) and mouse anti-Calbindin-D28k monoclonal antibody (66394-1-Ig, 1:200, green). Multi-rAb CoraLite® Plus 555 conjugated Recombinant Goat anti-rabbit secondary antibody (RGAR003, 1:500) and Multi-rAb CoraLite® Plus 488 conjugated Goat Anti-Mouse Recombinant Secondary Antibody (H+L) were used for detection (RGAM002, 1:500) .

Immunofluorescence of rat brain: rat brain FFPE section was stained with Rabbit anti-GFAP polyclonal antibody (16825-1-AP, 1:200, orange) and mouse anti-NeuN monoclonal antibody (66836-1-Ig, green). Multi-rAb CoraLite® Plus 555 conjugated Recombinant Goat anti-rabbit secondary antibody (RGAR003, 1:500) and Multi-rAb CoraLite® Plus 488 conjugated Goat Anti-Mouse Recombinant Secondary Antibody (H+L) were used for detection (RGAM002, 1:500) .

Immunofluorescence of Hela cells: Hela cells were fixed with 4% PFA and stained with Rabbit anti-TOM70 polyclonal antibody (14528-1-AP, 1:200) Multi-rAb CoraLite® Plus 555 conjugated Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR003, 1:600) was used for detection. Nucleus was stained with DAPI(blue). The experiment was performed in Chromotek’s lab and image was recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescent analysis of (4% PFA) fixed OCT-embedded frozen mouse small intestine tissue using smooth muscle actin antibody (14395-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 555-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR003).

Immunofluorescent analysis of (4% PFA) fixed OCT-embedded frozen mouse colon tissue using E-cadherin antibody (20874-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 555-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR003).

Immunofluorescence of MCF-7 cells: MCF-7 cells were fixed with 4% PFA and stained with Rabbit anti-ZO1 polyclonal antibody (21773-1-AP, 1:2000, orange) and mouse anti-Alpha Tubulin monoclonal antibody (66031-1-Ig, 1:1000, green). Multi-rAb CoraLite® Plus 555-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR003, 1:500) and Multi-rAb CoraLite® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002, 1:500) were used for detection.

1X10^6 MOLT4 were surface stained with 0.2 ug Anti-HLA class I rabbit polyclonal antibody (15240-1-AP) and Rabbit IgG Isotype Control 30000-0-AP. Multi-rAb CoraLite® Plus 550-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) RGAR003 was used at 1:500 for detection.

Immunofluorescence of MCF-7 cells: MCF-7 cells were fixed with 4% PFA and stained with Rabbit anti-ZO1 polyclonal antibody (21773-1-AP, 1:2000, green) and mouse anti-Alpha Tubulin monoclonal antibody (66031-1-Ig, 1:1000, orange). Multi-rAb CoraLite® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002, 1:500) and Multi-rAb CoraLite® Plus 555-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM003, 1:500) were used for detection.

Immunofluorescence of Hela cells: Hela cells were fixed with 4% PFA and stained with Rabbit anti-Alpha Tubulin polyclonal antibody (11224-1-AP, 1:200, green) and mouse anti-NPM1 monoclonal antibody (60096-1-Ig, 1:1000, orange). Multi-rAb CoraLite® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002, 1:500) and Multi-rAb CoraLite® Plus 555-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM003, 1:500) were used for detection.

Immunofluorescence of mouse cerebellum: mouse cerebellum FFPE section was stained with Rabbit anti-NeuN polyclonal antibody (26975-1-AP, 1:200, green) and mouse anti-Calbindin-D28k monoclonal antibody (66394-1-Ig, 1:200, orange). Multi-rAb CoraLite® Plus 488 conjugated Recombinant Goat anti-rabbit secondary antibody (RGAR002, 1:500) and Multi-rAb CoraLite® Plus 555 conjugated Recombinant Goat anti-mouse secondary antibody were used for detection (RGAM003, 1:500) .

Immunofluorescence of rat brain: rat brain FFPE section was stained with Rabbit anti-GFAP polyclonal antibody (16825-1-AP, 1:200, green) and mouse anti-NeuN monoclonal antibody (66836-1-Ig, orange). Multi-rAb CoraLite® Plus 488 conjugated Recombinant Goat anti-rabbit secondary antibody (RGAR002, 1:500) and Multi-rAb CoraLite® Plus 555 conjugated Goat Anti-Mouse Recombinant Secondary Antibody (H+L) were used for detection (RGAM003, 1:500) .

Immunofluorescent analysis of (4% PFA) fixed OCT-embedded frozen mouse heart tissue using ACTC1-specific antibody (66125-1-Ig, Clone: 1F2B9 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 555-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM003).

1X10^6 MOLT4 were surface stained with 0.2 ug Anti-Human CD8 (65204-1-Ig, Clone: UCHT4) and Mouse IgG2a Isotype Control 66360-2-Ig. Multi-rAb CoraLite® Plus 550-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) RGAM003 was used at 1:500 for detection.

Immunofluorescence of Hela cells: Hela cells were fixed with 4% PFA and stained with Rabbit anti-Alpha Tubulin polyclonal antibody (11224-1-AP, 1:200, magenta) and mouse anti-NPM1 monoclonal antibody (60096-1-Ig, 1:1000, green). Multi-rAb CoraLite® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005, 1:500) and Multi-rAb CoraLite® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002, 1:500) were used for detection.

Immunofluorescence of mouse cerebellum: mouse cerebellum FFPE section was stained with Rabbit anti-NeuN polyclonal antibody (26975-1-AP, 1:200, magenta) and mouse anti-Calbindin-D28k monoclonal antibody (66394-1-Ig, 1:200, green). Multi-rAb CoraLite® Plus 647 conjugated Recombinant Goat anti-rabbit secondary antibody (RGAR005, 1:500) and Multi-rAb CoraLite® Plus 488 conjugated Goat Anti-Mouse Recombinant Secondary Antibody (H+L) were used for detection (RGAM002, 1:500) .

Immunofluorescence of rat brain: rat brain FFPE section was stained with Rabbit anti-GFAP polyclonal antibody (16825-1-AP, 1:200, magenta) and mouse anti-NeuN monoclonal antibody (66836-1-Ig, green). Multi-rAb CoraLite® Plus 647 conjugated Recombinant Goat anti-rabbit secondary antibody (RGAR005, 1:500) and Multi-rAb CoraLite® Plus 488 conjugated Goat Anti-Mouse Recombinant Secondary Antibody (H+L) were used for detection (RGAM002, 1:500) .

Immunofluorescence of Hela cells: Hela cells were fixed with 4% PFA and stained with Rabbit anti-GM130 polyclonal antibody (11308-1-AP, 1:200) Multi-rAb CoraLite® Plus 647 conjugated Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005,1:800) was used for detection. Nucleus was stained with DAPI (blue).    The experiment was performed in Chromotek’s lab and image was recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescent analysis of (4% PFA) fixed OCT-embedded frozen mouse small intestine tissue using smooth muscle actin antibody (14395-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005).

Immunofluorescent analysis of (4% PFA) fixed OCT-embedded frozen mouse colon tissue using E-cadherin antibody (20874-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005).

Immunofluorescence of MCF-7 cells: MCF-7 cells were fixed with 4% PFA and stained with Rabbit anti-ZO1 polyclonal antibody (21773-1-AP, 1:2000, magenta) and mouse anti-Alpha Tubulin monoclonal antibody (66031-1-Ig, 1:1000, green). Multi-rAb CoraLite® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005, 1:500) and Multi-rAb CoraLite® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002, 1:500) were used for detection.

1X10^6 MOLT4 were surface stained with 0.2 ug Anti-HLA class I rabbit polyclonal antibody (15240-1-AP) and Rabbit IgG Isotype Control 30000-0-AP. Multi-rAb CoraLite® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) RGAR005 was used at 1:500 for detection.

Various lysates were subjected to SDS-PAGE followed by western blot with mouse anti-Beta Tubulin monoclonal antibody (Cat.NO. 66240-1-Ig, isotype IgG2a). Multi-rAb CoraLite® Plus 750-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) RGAM006 was used at 1:10000 for detection. Different devices were used for imaging for the same membrane.

Various lysates were subjected to SDS-PAGE followed by western blot with mouse anti-ZNF174 monoclonal antibody (Cat.NO. 68426-1-Ig, isotype IgG3). Multi-rAb CoraLite® Plus 750-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) RGAM006 was used at 1:10000 for detection. Different devices were used for imaging for the same membrane.

Various lysates were subjected to SDS-PAGE followed by western blot with mouse anti-EIF3E monoclonal antibody (Cat.NO. 67095-1-Ig, isotype IgG1) at dilution of 1:50000. Multi-rAb CoraLite® Plus 750-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) RGAM006 was used at 1:10000 for detection.

Various lysates were subjected to SDS-PAGE followed by western blot with mouse anti-Catalase monoclonal antibody (Cat.NO. 66765-1-Ig, isotype IgG1) at dilution of 1:10000. Multi-rAb CoraLite® Plus 750-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) RGAM006 was used at 1:10000 for detection.

Various lysates were subjected to SDS-PAGE followed by western blot with mouse anti-Beta Tubulin monoclonal antibody (Cat.NO. 66240-1-Ig, isotype IgG2a) at dilution of 1:100000. Multi-rAb CoraLite® Plus 750-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) RGAM006 was used at 1:10000 for detection.

Various lysates were subjected to SDS-PAGE followed by western blot with mouse anti-FAK monoclonal antibody (Cat.NO. 66258-1-Ig, isotype IgG2c) at dilution of 1:5000. Multi-rAb CoraLite® Plus 750-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) RGAM006 was used at 1:10000 for detection.

Various lysates were subjected to SDS-PAGE followed by western blot with mouse anti-ZNF174 monoclonal antibody (Cat.NO. 68426-1-Ig, isotype IgG3) at dilution of 1:2000. Multi-rAb CoraLite® Plus 750-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) RGAM006 was used at 1:10000 for detection.

Immunofluorescence of A431 cells: PFA-fixed cells were stained with anti-B23 (60096-1-Ig), followed by Multi-rAb CoraLite® Plus 750-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM006, 1:500, red), nuclei stained with DAPI (cyan). Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich. Device setting for target: Laser Ex max 764nm, filter Em max 775nm.

Immunofluorescence of A431 cells: PFA-fixed cells were co-stained with mouse anti-B23 (60096-1-Ig) and rabbit anti-Lamin B1 (12987-1-AP), followed by Multi-rAb CoraLite® Plus 750-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM006, 1:500, red) and Nano-Secondary srb2GCL555-1 (yellow). Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich. Device setting for RGAM006: Laser Ex max 764nm, filter Em max 775nm.

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 750-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM006), CL488-Phalloidin (green).

1X10^6 MOLT4 were surface stained with 0.2 ug Anti-Human CD8 (65204-1-Ig, Clone: UCHT4) and Mouse IgG2a Isotype Control 66360-2-Ig. Multi-rAb CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) RGAM006 was used at 1:500 for detection.

Various lysates were subjected to SDS-PAGE followed by western blot with Gelsolin mouse monoclonal antibody (66280-1-Ig, IgA). HRP-Goat Anti-Mouse IgA-Specific Recombinant Secondary Antibody (RGAM801) and Multi-rAb HRP-Goat Anti-Mouse IgG (H+L) Recombinant Secondary Antibody (RGAM001) were used at 1: 20000 for detection. Note that a higher signal is obtained using RGAM001 as it recognizes multiple epitopes.

Various lysates were subjected to SDS-PAGE followed by western blot with NRF1 mouse monoclonal antibody (66832-1-Ig, IgG2b). HRP-Goat Anti-Mouse IgA-Specific Recombinant Secondary Antibody (RGAM801) and Multi-rAb HRP-Goat Anti-Mouse IgG (H+L) Recombinant Secondary Antibody (RGAM001) were used at 1: 20000 for detection. The result shows that RGAM801 does not react with mouse IgG2b.

Various lysates were subjected to SDS-PAGE followed by western blot with EIF3E mouse monoclonal antibody (67095-1-Ig, IgG1). HRP-Goat Anti-Mouse IgA-Specific Recombinant Secondary Antibody (RGAM801) and Multi-rAb HRP-Goat Anti-Mouse IgG (H+L) Recombinant Secondary Antibody (RGAM001) were used at 1: 20000 for detection. The result shows that RGAM801 does not react with mouse IgG1.

Various lysates were subjected to SDS-PAGE followed by western blot with ZNF174 mouse monoclonal antibody (68426-1-Ig, IgG3). HRP-Goat Anti-Mouse IgA-Specific Recombinant Secondary Antibody (RGAM801) and Multi-rAb HRP-Goat Anti-Mouse IgG (H+L) Recombinant Secondary Antibody (RGAM001) were used at 1: 20000 for detection. The result shows that RGAM801 does not react with mouse IgG3.

Various lysates were subjected to SDS-PAGE followed by western blot with Actin mouse monoclonal antibody (60008-1-Ig, IgM). HRP-Goat Anti-Mouse IgA-Specific Recombinant Secondary Antibody (RGAM801) and Multi-rAb HRP-Goat Anti-Mouse IgG (H+L) Recombinant Secondary Antibody (RGAM001) were used at 1: 20000 for detection. The result shows that RGAM801 does not react with mouse IgM.

Various lysates were subjected to SDS-PAGE followed by western blot with Beta-Tubulin mouse monoclonal antibody (66240-1-Ig, IgG2a). HRP-Goat Anti-Mouse IgA-Specific Recombinant Secondary Antibody (RGAM801) and Multi-rAb HRP-Goat Anti-Mouse IgG (H+L) Recombinant Secondary Antibody (RGAM001) were used at 1: 20000 for detection. The result shows that RGAM801 does not react with mouse IgG2a.

Various lysates were subjected to SDS-PAGE followed by western blot with FAK mouse monoclonal antibody (66258-1-Ig, IgG2c). HRP-Goat Anti-Mouse IgA-Specific Recombinant Secondary Antibody (RGAM801) and Multi-rAb HRP-Goat Anti-Mouse IgG (H+L) Recombinant Secondary Antibody (RGAM001) were used at 1: 20000 for detection. The result shows that RGAM801 does not react with mouse IgG2c.

Direct ELISA was performed by coating mouse monoclonal antibodies with different isotypes followed by signal development using HRP-Goat Anti-Mouse IgA-Specific Recombinant Secondary Antibody (RGAM801). The result indicates that RGAM801 strongly binds to Mouse IgA and does not react with Mouse IgGs or mouse IgM.

Various lysates were subjected to SDS-PAGE followed by western blot with Actin mouse monoclonal antibody (60008-1-Ig, IgM). HRP-Goat Anti-Mouse IgM-Specific Recombinant Secondary Antibody (RGAM701) and Multi-rAb HRP-Goat Anti-Mouse IgG (H+L) Recombinant Secondary Antibody (RGAM001) were used at 1: 20000 for detection. Note that a higher signal is obtained using RGAM001 as it recognizes multiple epitopes.

Various lysates were subjected to SDS-PAGE, followed by western blot with Beta-Tubulin mouse monoclonal antibody (66240-1-Ig, IgG2a). HRP-Goat Anti-Mouse IgM-Specific Recombinant Secondary Antibody (RGAM701) and Multi-rAb HRP-Goat Anti-Mouse IgG (H+L) Recombinant Secondary Antibody (RGAM001) were used at 1: 20000 for detection. The result shows that RGAM701 does not react with mouse IgG2a.

Various lysates were subjected to SDS-PAGE followed by western blot with FAK mouse monoclonal antibody (66258-1-Ig, IgG2c). HRP-Goat Anti-Mouse IgM-Specific Recombinant Secondary Antibody (RGAM701) and Multi-rAb HRP-Goat Anti-Mouse IgG (H+L) Recombinant Secondary Antibody (RGAM001) were used at 1: 20000 for detection. The result shows that RGAM701 does not react with mouse IgG2c.

Various lysates were subjected to SDS-PAGE followed by western blot with Gelsolin mouse monoclonal antibody (66280-1-Ig, IgA). HRP-Goat Anti-Mouse IgM-Specific Recombinant Secondary Antibody (RGAM701) and Multi-rAb HRP-Goat Anti-Mouse IgG (H+L) Recombinant Secondary Antibody (RGAM001) were used at 1: 20000 for detection. The result shows that RGAM701 does not react with mouse IgA.

Various lysates were subjected to SDS-PAGE, followed by western blot with NRF1 mouse monoclonal antibody (66832-1-Ig, IgG2b). HRP-Goat Anti-Mouse IgM-Specific Recombinant Secondary Antibody (RGAM701) and Multi-rAb HRP-Goat Anti-Mouse IgG (H+L) Recombinant Secondary Antibody (RGAM001) were used at 1: 20000 for detection. The result shows that RGAM701 does not react with mouse IgG2b.

Various lysates were subjected to SDS-PAGE followed by western blot with EIF3E mouse monoclonal antibody (67095-1-Ig, IgG1). HRP-Goat Anti-Mouse IgM-Specific Recombinant Secondary Antibody (RGAM701) and Multi-rAb HRP-Goat Anti-Mouse IgG (H+L) Recombinant Secondary Antibody (RGAM001) were used at 1: 20000 for detection. The result shows that RGAM701 does not react with mouse IgG1.

Various lysates were subjected to SDS-PAGE followed by western blot with ZNF174 mouse monoclonal antibody (68426-1-Ig, IgG3). HRP-Goat Anti-Mouse IgM-Specific Recombinant Secondary Antibody (RGAM701) and Multi-rAb HRP-Goat Anti-Mouse IgG (H+L) Recombinant Secondary Antibody (RGAM001) were used at 1: 20000 for detection. The result shows that RGAM701 does not react with mouse IgG3.

Direct ELISA was performed by coating mouse monoclonal antibodies with different isotypes followed by signal development using HRP-Goat Anti-Mouse IgM-Specific Recombinant Secondary Antibody (RGAM701). The result indicates that RGAM701 strongly binds to Mouse IgM and does not react with Mouse IgGs or mouse IgA.

Immunofluorescence of MCF-7 cells: MCF-7 cells were fixed with 4% PFA and stained with Rabbit anti-ZO1 polyclonal antibody (21773-1-AP, 1:2000, green) and mouse anti-Alpha Tubulin monoclonal antibody (66031-1-Ig, 1:1000, magenta). Multi-rAb CoraLite® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002, 1:500) and Multi-rAb CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM005, 1:500) were used for detection.

Immunofluorescent analysis of (4% PFA) fixed OCT-embedded frozen mouse heart tissue using ACTC1-specific antibody (66125-1-Ig, Clone: 1F2B9 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM005).

Immunofluorescence of Hela cells: Hela cells were fixed with 4% PFA and stained with Rabbit anti-Alpha Tubulin polyclonal antibody (11224-1-AP, 1:200, green) and mouse anti-NPM1 monoclonal antibody (60096-1-Ig, 1:1000, magenta). Multi-rAb CoraLite® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002, 1:500) and Multi-rAb CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM005, 1:500) were used for detection.

Immunofluorescence of mouse cerebellum: mouse cerebellum FFPE section was stained with Rabbit anti-NeuN polyclonal antibody (26975-1-AP, 1:200, green) and mouse anti-Calbindin-D28k monoclonal antibody (66394-1-Ig, 1:200, magenta). Multi-rAb CoraLite® Plus 488 conjugated Recombinant Goat anti-rabbit secondary antibody (RGAR002, 1:500) and Multi-rAb CoraLite® Plus 647 conjugated Goat Anti-Mouse Recombinant Secondary Antibody (H+L) were used for detection (RGAM005, 1:500) .

Immunofluorescence of rat brain: rat brain FFPE section was stained with Rabbit anti-GFAP polyclonal antibody (16825-1-AP, 1:200, green) and mouse anti-NeuN monoclonal antibody (66836-1-Ig, magenta). Multi-rAb CoraLite® Plus 488 conjugated Recombinant Goat anti-rabbit secondary antibody (RGAR002, 1:500) and Multi-rAb CoraLite® Plus 647 conjugated Goat Anti-Mouse Recombinant Secondary Antibody (H+L) were used for detection (RGAM005, 1:500) .

1X10^6 MOLT4 were surface stained with 0.2 ug Anti-Human CD8 (65204-1-Ig, Clone: UCHT4) and Mouse IgG2a Isotype Control 66360-2-Ig. Multi-rAb CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) RGAM005 was used at 1:500 for detection.

Various lysates were subjected to SDS-PAGE followed by western blot with EIF3E mouse monoclonal antibody (67095-1-Ig, left panel) and Beta-actin mouse monoclonal antibody (60008-1-Ig, right panel). Multi-rAb HRP-Goat Anti-Mouse IgG1-Specific Recombinant Secondary Antibody were used at 1:20000 for detection. The result shows that the secondary does not react with mouse IgM.

Various lysates were subjected to SDS-PAGE followed by western blot with EIF3E mouse monoclonal antibody (67095-1-Ig, mouse IgG1 subclass, left panel) and Gelsolin mouse monoclonal antibody (66280-1-Ig, mouse IgA class, right panel). Multi-rAb HRP-Goat Anti-Mouse IgG1-Specific Recombinant Secondary Antibody were used at 1:20000 for detection. The result shows that the secondary does not react with mouse IgA.

Indirect ELISA was performed by coating ag26747 (GPNMB fusion protein) at 70 ng/well, ag0297 (beta actin fusion protein), and ag17916 (Gelsolin fusion protein) at 140ng/well. Corresponding mouse monoclonal antibodies 66926-1-Ig (IgG class), 60008-1-Ig (IgM class), and 66280-1-Ig (IgA class) were used as primary antibodies at 0.1μg/mL. 0.125 μg/mL Multi-rAb HRP-Goat Anti-Mouse IgG-Specific Recombinant Secondary Antibody (RGAM601) was used for detection. The result indicates that RGAM601 strongly binds to Mouse IgG and does not react with Mouse IgM or IgA.