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Multi-rAb Polymer HRP-Goat Anti-Rabbit/Mouse Universal Recombinant Secondary Antibody (H+L)

Multi-rAb Goat Anti-Rabbit/Mouse Universal Recombinant Secondary Antibody is a mixture of precisely engineered recombinant monoclonal antibodies that recognize multiple complementary epitopes on rabbit/mouse IgG heavy and light chain.

Applications

IHC

Conjugate

Polymer HRP

Host

Goat

Reactivity

Rabbit,Mouse

Cat no : RGAU011

Validation Data Gallery

  • Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using commercial IVD grade pan-CK mouse monoclonal antibody. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat Anti-Rabbit/Mouse Universal Recombinant Secondary Antibody (H+L) RGAU011 was used for detection.

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using commercial IVD grade pan-CK mouse monoclonal antibody. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat Anti-Rabbit/Mouse Universal Recombinant Secondary Antibody (H+L) RGAU011 was used for detection.

  • Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using other vendor's BCL2 rabbit recombinant antibody. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat Anti-Rabbit/Mouse Universal Recombinant Secondary Antibody (H+L) RGAU011 was used for detection.

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using other vendor's BCL2 rabbit recombinant antibody. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat Anti-Rabbit/Mouse Universal Recombinant Secondary Antibody (H+L) RGAU011 was used for detection.

  • Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 80232-1-RR (Vimentin rabbit recombinant antibody) at dilution of 1:2500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-Rabbit/Mouse Universal Recombinant Secondary Antibody RGAU011 was used for detection. The staining was performed on Celnovte's CNT360-M1 autostainer.

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 80232-1-RR (Vimentin rabbit recombinant antibody) at dilution of 1:2500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-Rabbit/Mouse Universal Recombinant Secondary Antibody RGAU011 was used for detection. The staining was performed on Celnovte's CNT360-M1 autostainer.

  • Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66379-1-Ig (Beta catennin mouse monoclonal recombinant antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-Rabbit/Mouse Universal Recombinant Secondary Antibody RGAU011 was used for detection. The staining was performed on Celnovte's CNT360-M1 autostainer.

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66379-1-Ig (Beta catennin mouse monoclonal recombinant antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-Rabbit/Mouse Universal Recombinant Secondary Antibody RGAU011 was used for detection. The staining was performed on Celnovte's CNT360-M1 autostainer.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 80001-1-RR (TDP-43 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat Anti-Rabbit/Mouse Universal Recombinant secondary antibody RGAU011 was used for detection.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 80001-1-RR (TDP-43 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat Anti-Rabbit/Mouse Universal Recombinant secondary antibody RGAU011 was used for detection.

  • Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 12715-1-AP (Dystrophin antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat Anti-Rabbit/Mouse Universal Recombinant secondary antibody RGAU011 was used for detection.

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 12715-1-AP (Dystrophin antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat Anti-Rabbit/Mouse Universal Recombinant secondary antibody RGAU011 was used for detection.

  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 81668-1-RR (F4/80 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat Anti-Rabbit/Mouse Universal Recombinant secondary antibody RGAU011 was used for detection.

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 81668-1-RR (F4/80 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat Anti-Rabbit/Mouse Universal Recombinant secondary antibody RGAU011 was used for detection.

  • Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 66379-1-Ig (Beta Catenin antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat Anti-Rabbit/Mouse Universal Recombinant secondary antibody RGAU011 was used for detection.

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 66379-1-Ig (Beta Catenin antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat Anti-Rabbit/Mouse Universal Recombinant secondary antibody RGAU011 was used for detection.

  • Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66836-1-Ig (NeuN antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat Anti-Rabbit/Mouse Universal Recombinant secondary antibody RGAU011 was used for detection.

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66836-1-Ig (NeuN antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat Anti-Rabbit/Mouse Universal Recombinant secondary antibody RGAU011 was used for detection.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66177-1-Ig (MPO antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat Anti-Rabbit/Mouse Universal Recombinant secondary antibody RGAU011 was used for detection.

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66177-1-Ig (MPO antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat Anti-Rabbit/Mouse Universal Recombinant secondary antibody RGAU011 was used for detection.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66786-1-Ig (SOX10 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat Anti-Rabbit/Mouse Universal Recombinant secondary antibody RGAU011 was used for detection.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66786-1-Ig (SOX10 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat Anti-Rabbit/Mouse Universal Recombinant secondary antibody RGAU011 was used for detection.

  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67300-1-Ig (CD107a / LAMP1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat Anti-Rabbit/Mouse Universal Recombinant secondary antibody RGAU011 was used for detection.

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67300-1-Ig (CD107a / LAMP1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat Anti-Rabbit/Mouse Universal Recombinant secondary antibody RGAU011 was used for detection.

  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 67479-1-Ig (SLC22A7 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat Anti-Rabbit/Mouse Universal Recombinant secondary antibody RGAU011 was used for detection.

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 67479-1-Ig (SLC22A7 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat Anti-Rabbit/Mouse Universal Recombinant secondary antibody RGAU011 was used for detection.

Product Information

RGAU011 targets Mouse IgG (H+L) and Rabbit IgG (H+L) in IHC. It is higly specific to mouse IgG and rabbit IgG and shows minimal reactivity for human, pig and bovine IgGs.

Concentration Ready to use
Applications IHC
Host Species Goat
Species Reactivity Rabbit,Mouse
Physical State Liquid
Suggested Dilution Range Ready to use
Purity Protein G purification
Storage Buffer TBST+10 mg/mL BSA+1 mg/mL gelatin + 0.1% proclin300
Storage Instructions Store at 2-8°C. Stable for one year after shipment.
ClonalityMulticlonal recombinant
RRIDAB_3101771
NoteThis product is for research use only, not for diagnostic or therapeutic use.

Background

Multi-rAb Recombinant Secondary Antibodies are mixtures of precisely engineered recombinant monoclonal antibodies that recognize multiple complementary epitopes on the same IgG. Each recombinant clone in the final multiclonal mixture is carefully selected after rigorous characterization and screening to ensure the highest level of performance. ​The selected clones are mixed in various combinations, each of which is then functionally characterized in multiple applications to select the final multiclonal mixture with optimal performance. Each lot of Multi-rAb Secondary Antibodies is produced by mixing the same high performing recombinant clones every time to ensure that they have high batch-to-batch consistency, which in turn results in high reproducibility of experimental data.
Multi-rAb Polymer HRP-Goat Anti-Rabbit/Mouse Universal Recombinant Secondary Antibody (H+L) enables highly sensitive, biotin-free detection of rabbit/mouse primary antibodies in IHC. Unlike traditional avidin-biotin-based signal amplification methods, polymer HRP-based detection offers the advantages of a lower background and faster protocol with fewer steps.
Moreover, Multi-rAb Polymer HRP secondary antibodies are provided in a ready-to-use format, in easy-to-use dropper bottles making it extremely convenient for users to incorporate them into their IHC staining protocols.

Publications

Title

J Chin Med Assoc

WGCNA reveals a biomarker for cancer-associated fibroblasts to predict prognosis in cervical cancer

Authors - Zao-Ling Liu
View Article