Multi-rAb Polymer HRP-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)

Multi-rAb Goat Anti-mouse recombinant secondary antibody is a mixture of precisely engineered recombinant monoclonal antibodies that recognize multiple complementary epitopes on mouse IgG heavy and light chain.

Applications

IHC

Conjugate

Polymer HRP

Host

Goat

Reactivity

Mouse

Publications

4

Cat no : RGAM011

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Validation Data Gallery

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  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66096-1-Ig (Villin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-Mouse Recombinant secondary antibody RGAM011 was used for detection.

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  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66065-2-Ig (CD31 mouse monoclonal antibody) at dilution of 1:10000 . Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-mouse Recombinant secondary antibody RGAM011 was used for detection.

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  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66379-1-Ig (Beta Catenin mouse monoclonal antibody) at dilution of 1:20000 . Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-mouse Recombinant secondary antibody RGAM011 was used for detection.

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  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using commercial IVD grade pan-CK mouse monoclonal antibody. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-mouse Recombinant secondary antibody RGAM011 was used for detection.

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  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using other vendor's NeuN mouse monoclonal antibody. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-mouse Recombinant secondary antibody RGAM011 was used for detection.

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  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 67479-1-Ig (SLC22A7 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-Mouse Recombinant secondary antibody RGAM011 was used for detection. The staining was performed on Celnovte's CNT360-M1 autostainer.

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  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66836-1-Ig (NeuN antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-Mouse Recombinant secondary antibody RGAM011 was used for detection. The staining was performed on Celnovte's CNT360-M1 autostainer.

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  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66177-1-Ig (MPO antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-Mouse Recombinant secondary antibody RGAM011 was used for detection. The staining was performed on Celnovte's CNT360-M1 autostainer.

Product Information

RGAM011 targets Mouse IgG (H+L) in IHC. It can bind to all mouse IgG isotypes, mouse IgM and IgA as well as rat IgG. It shows minimal reactivity for rabbit, human, pig and bovine IgGs.

• Concentration: Ready to use
• Applications: IHC
• Host Species: Goat
• Species Reactivity: Mouse
• Physical State: Liquid
• Suggested Dilution Range: Ready to use
• Purity: Protein G purification
• Storage Buffer: TBST+10 mg/mL BSA+1 mg/mL gelatin + 0.1% proclin300
• Storage Instructions: Store at 2-8°C. Stable for one year after shipment.
• Clonality: Multiclonal recombinant
• RRID: AB_3094533
• Note: This product is for research use only,not for diagnostic or therapeutic use.

Background

Multi-rAb Recombinant Secondary Antibodies are mixtures of precisely engineered recombinant monoclonal antibodies that recognize multiple complementary epitopes on the same IgG. Each recombinant clone in the final multiclonal mixture is carefully selected after rigorous characterization and screening to ensure the highest level of performance. ​The selected clones are mixed in various combinations, each of which is then functionally characterized in multiple applications to select the final multiclonal mixture with optimal performance. Each lot of Multi-rAb Secondary Antibodies is produced by mixing the same high performing recombinant clones every time to ensure that they have high batch-to-batch consistency, which in turn results in high reproducibility of experimental data.
Multi-rAb Polymer HRP-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) enables highly sensitive, biotin-free detection of mouse primary antibodies in IHC. Unlike traditional avidin-biotin-based signal amplification methods, polymer HRP-based detection offers the advantages of a lower background and faster protocol with fewer steps.Moreover, Multi-rAb Polymer HRP secondary antibodies are provided in a ready-to-use format, in easy-to-use dropper bottles making it extremely convenient for users to incorporate them into their IHC staining protocols.

Publications

Title
Biomed Pharmacother; Host-microbiota interactions in collagen-induced arthritis rats treated with human umbilical cord mesenchymal stem cell exosome and ginsenoside Rh2; Authors - Zhongsheng Zhou
Gene; miR-744-5p promotes T-cell differentiation via inhibiting STK11; Authors - Jiayi Han
Oncogene; CircVPS8 promotes the malignant phenotype and inhibits ferroptosis of glioma stem cells by acting as a scaffold for MKRN1, SOX15 and HNF4A; Authors - Jinpeng Hu
J Appl Microbiol; Forsythoside B, the active component of Frosythiae fructuse water extract, alleviates Streptococcus pneumoniae virulence by targeting pneumolysin; Authors - Zhongtian Wang

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