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HRP-Goat Anti-Mouse IgM-Specific Recombinant Secondary Antibody

HRP-Goat Anti-Mouse IgM-Specific Recombinant Secondary Antibody is a precisely engineered recombinant monoclonal antibody.

Applications

ELISA, WB, Dot blot

Conjugate

HRP

Host

Goat

Reactivity

Mouse

Cat no : RGAM701

Validation Data Gallery

  • Various lysates were subjected to SDS-PAGE followed by western blot with Actin mouse monoclonal antibody (60008-1-Ig, IgM). HRP-Goat Anti-Mouse IgM-Specific Recombinant Secondary Antibody (RGAM701) and Multi-rAb HRP-Goat Anti-Mouse IgG (H+L) Recombinant Secondary Antibody (RGAM001) were used at 1: 20000 for detection. Note that a higher signal is obtained using RGAM001 as it recognizes multiple epitopes.

    Various lysates were subjected to SDS-PAGE followed by western blot with Actin mouse monoclonal antibody (60008-1-Ig, IgM). HRP-Goat Anti-Mouse IgM-Specific Recombinant Secondary Antibody (RGAM701) and Multi-rAb HRP-Goat Anti-Mouse IgG (H+L) Recombinant Secondary Antibody (RGAM001) were used at 1: 20000 for detection. Note that a higher signal is obtained using RGAM001 as it recognizes multiple epitopes.

  • Various lysates were subjected to SDS-PAGE, followed by western blot with Beta-Tubulin mouse monoclonal antibody (66240-1-Ig, IgG2a). HRP-Goat Anti-Mouse IgM-Specific Recombinant Secondary Antibody (RGAM701) and Multi-rAb HRP-Goat Anti-Mouse IgG (H+L) Recombinant Secondary Antibody (RGAM001) were used at 1: 20000 for detection. The result shows that RGAM701 does not react with mouse IgG2a.

    Various lysates were subjected to SDS-PAGE, followed by western blot with Beta-Tubulin mouse monoclonal antibody (66240-1-Ig, IgG2a). HRP-Goat Anti-Mouse IgM-Specific Recombinant Secondary Antibody (RGAM701) and Multi-rAb HRP-Goat Anti-Mouse IgG (H+L) Recombinant Secondary Antibody (RGAM001) were used at 1: 20000 for detection. The result shows that RGAM701 does not react with mouse IgG2a.

  • Various lysates were subjected to SDS-PAGE followed by western blot with FAK mouse monoclonal antibody (66258-1-Ig, IgG2c). HRP-Goat Anti-Mouse IgM-Specific Recombinant Secondary Antibody (RGAM701) and Multi-rAb HRP-Goat Anti-Mouse IgG (H+L) Recombinant Secondary Antibody (RGAM001) were used at 1: 20000 for detection. The result shows that RGAM701 does not react with mouse IgG2c.

    Various lysates were subjected to SDS-PAGE followed by western blot with FAK mouse monoclonal antibody (66258-1-Ig, IgG2c). HRP-Goat Anti-Mouse IgM-Specific Recombinant Secondary Antibody (RGAM701) and Multi-rAb HRP-Goat Anti-Mouse IgG (H+L) Recombinant Secondary Antibody (RGAM001) were used at 1: 20000 for detection. The result shows that RGAM701 does not react with mouse IgG2c.

  • Various lysates were subjected to SDS-PAGE followed by western blot with Gelsolin mouse monoclonal antibody (66280-1-Ig, IgA). HRP-Goat Anti-Mouse IgM-Specific Recombinant Secondary Antibody (RGAM701) and Multi-rAb HRP-Goat Anti-Mouse IgG (H+L) Recombinant Secondary Antibody (RGAM001) were used at 1: 20000 for detection. The result shows that RGAM701 does not react with mouse IgA.

    Various lysates were subjected to SDS-PAGE followed by western blot with Gelsolin mouse monoclonal antibody (66280-1-Ig, IgA). HRP-Goat Anti-Mouse IgM-Specific Recombinant Secondary Antibody (RGAM701) and Multi-rAb HRP-Goat Anti-Mouse IgG (H+L) Recombinant Secondary Antibody (RGAM001) were used at 1: 20000 for detection. The result shows that RGAM701 does not react with mouse IgA.

  • Various lysates were subjected to SDS-PAGE, followed by western blot with NRF1 mouse monoclonal antibody (66832-1-Ig, IgG2b). HRP-Goat Anti-Mouse IgM-Specific Recombinant Secondary Antibody (RGAM701) and Multi-rAb HRP-Goat Anti-Mouse IgG (H+L) Recombinant Secondary Antibody (RGAM001) were used at 1: 20000 for detection. The result shows that RGAM701 does not react with mouse IgG2b.

    Various lysates were subjected to SDS-PAGE, followed by western blot with NRF1 mouse monoclonal antibody (66832-1-Ig, IgG2b). HRP-Goat Anti-Mouse IgM-Specific Recombinant Secondary Antibody (RGAM701) and Multi-rAb HRP-Goat Anti-Mouse IgG (H+L) Recombinant Secondary Antibody (RGAM001) were used at 1: 20000 for detection. The result shows that RGAM701 does not react with mouse IgG2b.

  • Various lysates were subjected to SDS-PAGE followed by western blot with ZNF174 mouse monoclonal antibody (68426-1-Ig, IgG3). HRP-Goat Anti-Mouse IgM-Specific Recombinant Secondary Antibody (RGAM701) and Multi-rAb HRP-Goat Anti-Mouse IgG (H+L) Recombinant Secondary Antibody (RGAM001) were used at 1: 20000 for detection. The result shows that RGAM701 does not react with mouse IgG3.

    Various lysates were subjected to SDS-PAGE followed by western blot with ZNF174 mouse monoclonal antibody (68426-1-Ig, IgG3). HRP-Goat Anti-Mouse IgM-Specific Recombinant Secondary Antibody (RGAM701) and Multi-rAb HRP-Goat Anti-Mouse IgG (H+L) Recombinant Secondary Antibody (RGAM001) were used at 1: 20000 for detection. The result shows that RGAM701 does not react with mouse IgG3.

  • Various lysates were subjected to SDS-PAGE followed by western blot with EIF3E mouse monoclonal antibody (67095-1-Ig, IgG1). HRP-Goat Anti-Mouse IgM-Specific Recombinant Secondary Antibody (RGAM701) and Multi-rAb HRP-Goat Anti-Mouse IgG (H+L) Recombinant Secondary Antibody (RGAM001) were used at 1: 20000 for detection. The result shows that RGAM701 does not react with mouse IgG1.

    Various lysates were subjected to SDS-PAGE followed by western blot with EIF3E mouse monoclonal antibody (67095-1-Ig, IgG1). HRP-Goat Anti-Mouse IgM-Specific Recombinant Secondary Antibody (RGAM701) and Multi-rAb HRP-Goat Anti-Mouse IgG (H+L) Recombinant Secondary Antibody (RGAM001) were used at 1: 20000 for detection. The result shows that RGAM701 does not react with mouse IgG1.

  • Direct ELISA was performed by coating mouse monoclonal antibodies with different isotypes followed by signal development using HRP-Goat Anti-Mouse IgM-Specific Recombinant Secondary Antibody (RGAM701). The result indicates that RGAM701 strongly binds to Mouse IgM and does not react with Mouse IgGs or mouse IgA.

    Direct ELISA was performed by coating mouse monoclonal antibodies with different isotypes followed by signal development using HRP-Goat Anti-Mouse IgM-Specific Recombinant Secondary Antibody (RGAM701). The result indicates that RGAM701 strongly binds to Mouse IgM and does not react with Mouse IgGs or mouse IgA.

Product Information

RGAM701 targets Mouse IgM in ELISA and Western blot. It can bind to Mouse IgM and does not react with Mouse IgG or IgA.

Concentration 1.0 mg/mL
Applications ELISA, WB, Dot blot
Host Species Goat
Species Reactivity Mouse
Physical State Liquid
Suggested Dilution Range 1:10,000-1:100,000 for ELISA
1:10,000-1:200,000 for western blotting with ECL substrates (1:10,000-1:40,000 is suggested for most systems).
Purity The antibody was purified from culture media supernatant by immunoaffinity chromatography using Protein G beads.
Storage Buffer PBS with 50% glycerol, 10 mg/mL BSA, 0.1% Proclin-300, pH 7.4.
Storage Instructions Store at -20°C. Stable for one year after shipment.
RRIDAB_3668657
NoteThis product is for research use only, not for diagnostic or therapeutic use.

Background

This secondary antibody reacts with Mouse IgM but does not react with Mouse IgG or IgA.