Alpha Tubulin Monoclonal antibody

Alpha Tubulin Monoclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, Canine and More (7)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1E4C11

Cat no : 66031-1-Ig

Synonyms

alpha Tubulin, Alpha tubulin ubiquitous, TUBA1B, tubulin, Tubulin alpha, tubulin, alpha 1b, α-tubulin


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Applications:
  • WB analysis using 66031-1-Ig

    WB analysis using 66031-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66031-1-Ig (Alpha Tubulin antibody) at dilution of 1:200000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 66031-1-Ig

    IP experiment of HeLa using 66031-1-Ig

    IP Result of anti-alpha Tubulin (IP:66031-1-Ig, 5ug; Detection:11224-1-AP 1:1000) with HeLa cells lysate 2800ug.

  • IHC staining of human tonsillitis using 66031-1-Ig

    IHC staining of human tonsillitis using 66031-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66031-1-Ig (alpha Tubulin antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 66031-1-Ig

    IHC staining of human colon cancer using 66031-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66031-1-Ig (Alpha Tubulin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 66031-1-Ig

    IHC staining of human colon cancer using 66031-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66031-1-Ig (Alpha Tubulin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 66031-1-Ig

    IHC staining of human liver cancer using 66031-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 66031-1-Ig(alpha Tubulin antibody) at dilution of 1:100 (under 40x lens).

  • IHC staining of human tonsillitis using 66031-1-Ig

    IHC staining of human tonsillitis using 66031-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66031-1-Ig (alpha Tubulin antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 66031-1-Ig

    IHC staining of human liver cancer using 66031-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 66031-1-Ig(alpha Tubulin antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human testis using 66031-1-Ig

    IHC staining of human testis using 66031-1-Ig

    Immunohistochemical analysis of paraffin-embedded human testis slide using 66031-1-Ig (alpha Tubulin antibody at dilution of 1:50.

  • IHC staining of human testis using 66031-1-Ig

    IHC staining of human testis using 66031-1-Ig

    Immunohistochemical analysis of paraffin-embedded human testis slide using 66031-1-Ig (alpha Tubulin antibody at dilution of 1:50.

  • IF Staining of HepG2 using 66031-1-Ig

    IF Staining of HepG2 using 66031-1-Ig

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed HepG2 cells using 66031-1-Ig(alpha Tubulin antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of rat brain using 66031-1-Ig

    IF Staining of rat brain using 66031-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using 66031-1-Ig (alpha Tubulin antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of HepG2 using 66031-1-Ig

    IF Staining of HepG2 using 66031-1-Ig

    Immunofluorescent analysis of HepG2 cells using 66031-1-Ig (alpha Tubulin antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

  • FC experiment of HeLa using 66031-1-Ig

    FC experiment of HeLa using 66031-1-Ig

    1X10^6 HeLa cells were stained with 0.05 ug Anti-Human Alpha Tubulin (66031-1-Ig, Clone:1E4C11) and FITC-Goat anti-mouse IgG at dilution 1:100 (red), or stained with 0.05 ug isotype control and FITC-Goat anti-mouse IgG at dilution 1:100 (blue). Cells were fixed with 90% MeOH and permeabilized with 0.1% TritonX-100.


Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman tonsillitis tissue, human testis tissue, human liver cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, rat brain tissue
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:200-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 66031-1-Ig is Alpha Tubulin Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat, Canine
Cited Reactivityhuman, mouse, rat, Bovine, canine, Drosophila, monkey, pig, rice, silkworm
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Alpha Tubulin fusion protein Ag18034
Full Name tubulin, alpha 1b
Calculated molecular weight 50 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC009314
Gene symbol TUBA1B
Gene ID (NCBI) 10376
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

What is the function of alpha tubulin?

Alpha-tubulin belongs to a large superfamily of tubulin proteins. There are a number of different subtypes that have a molecular weight of ~50kDa and are able to bind to beta-tubulin, forming a heterodimer that polymerises to microtubules as part of the cytoskeleton. These maintain cell structure, provide platforms for intracellular transport and are also involved in cell division.

 Where is alpha-tubulin expressed?

Alpha tubulin is highly conserved and is present in nearly all eukaryotic cells as one of the building blocks of microtubules. The ubiquitous nature of this protein has led to its common use as a control protein for many tissue types as well as highlighting the structure of the cytoskeleton. 

What are the post-translational modifications of alpha tubulin?

The function and properties of microtubules are drastically affected by the post-translational modifications undergone by tubulin, which may occur to the tubulin dimer directly or to the polymerised mictotubule. For example, the first modification to be identified was detyrosination1, as most alpha-tubulins have a tyrosine at their terminus. This process affects microtubules more than dimers and leads to patches of detyronisation along the structure, regulating protein interactions and allowing subcellular compartments to be defined.2,3  Polyglutamylation also occurs on several sites within the carboxy-terminal tails. However, to date, the most-studied alpha tubulin modification is related to acetylation of lysine 40 (K40).

 1. Gundersen, G. G., Khawaja, S. & Bulinski, J. C. Postpolymerization detyrosination of alpha-tubulin: a mechanism for subcellular differentiation of microtubules. J. Cell Biol. 105, 251-64 (1987).

2. Galjart, N. Plus-End-Tracking Proteins and Their Interactions at Microtubule Ends. Curr. Biol. 20, R528-R537 (2010).

3. Jiang, K. & Akhmanova, A. Microtubule tip-interacting proteins: a view from both ends. Curr. Opin. Cell Biol. 23, 94-101 (2011).


Protocols

Product Specific Protocols
WB protocol for Alpha Tubulin antibody 66031-1-IgDownload protocol
IHC protocol for Alpha Tubulin antibody 66031-1-IgDownload protocol
IF protocol for Alpha Tubulin antibody 66031-1-IgDownload protocol
IP protocol for Alpha Tubulin antibody 66031-1-IgDownload protocol
FC protocol for Alpha Tubulin antibody 66031-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Immunol

β-TrCP Restricts Lipopolysaccharide (LPS)-Induced Activation of TRAF6-IKK Pathway Upstream of IκBα Signaling.

Authors - Jin Liu
mouseIF

Hum Mol Genet

Nearly complete deletion of BubR1 causes microcephaly through shortened mitosis and massive cell death.

Authors - Ambrosia J Simmons
mouseWB

Dev Cell

Atoh1 Controls Primary Cilia Formation to Allow for SHH-Triggered Granule Neuron Progenitor Proliferation.

Authors - Chia-Hsiang Chang
humanWB

J Biol Chem

Legionella effector RavD binds phosphatidylinositol-3-phosphate and helps suppress endolysosomal maturation of the Legionella-containing vacuole.

Authors - Colleen M Pike
ratWB

Neurol Res

Neuroprotective effects of different frequency preconditioning exercise on neuronal apoptosis after focal brain ischemia in rats.

Authors - Takuto Terashi
humanIF

Int J Biochem Cell Biol

Glutamate affects the CYP1B1- and CYP2U1-mediated hydroxylation of arachidonic acid metabolism via astrocytic mGlu5 receptor.

Authors - Xuming Yu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Silvia (Verified Customer) (02-18-2022)

This antibody worked quite well in our western blotting and it comes at a great price.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: HeLa
FH

Azita (Verified Customer) (06-02-2021)

Immunohistochemistry labelling of (4% PFA) fixed mice spinal cord tissues using Alpha Tubulin Monoclonal Antibody at dilution of 1:50 showed strong labelling.

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:50
  • Cell Tissue Type: mice spinal cord tissues
FH

Scooby (Verified Customer) (10-05-2020)

happy

  • Applications: Immunofluorescence
  • Cell Tissue Type: n/a
FH

Lauren (Verified Customer) (10-05-2020)

thanks

  • Applications: Western Blot
FH

Chun (Verified Customer) (12-05-2019)

An excellent antibody

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:2,000
  • Cell Tissue Type: HeLa cells