CoraLite® Plus 488-conjugated Alpha Tubulin Monoclonal antibody

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• KD/KO Validated

Alpha Tubulin Monoclonal Antibody for WB, IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, canine

Applications

WB, IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Publications

5

CloneNo.

1E4C11

Cat no : CL488-66031

Synonyms

TUBA1B, Tubulin alpha 1B chain, Tubulin alpha, EC:3.6.5.-, Detyrosinated tubulin alpha-1B chain

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Validation Data Gallery

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  WB analysis using CL488-66031

  Various lysates were subjected to SDS PAGE followed by western blot with CL488-66031 (Alpha Tubulin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using CL488-66031

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using the CoraLite® Plus 488-conjugated version of this antibody, CL488-66031 (alpha Tubulin antibody), at dilution of 1:100.

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  IF Staining of mouse embryo using CL488-66031

  E14.5 FFPE mouse embryo section stained for smooth muscle actin (red, Cat. No 80008-1-RR) and alpha tubulin (green, Cat. CL488-66031). The alpha tubulin was conjugated to CoraLite-488 fluorescent dye. In red, you can see muscle tissue clearly stained including the tongue, heart, and arteries. Nerves and neurons are stained green since they are rich in tubulin. Image credit: @Immunofluorescence on Instagram

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  IF Staining of mouse embryo using CL488-66031

  E14.5 FFPE mouse embryo section stained for E-cadherin (red, Cat. No 20874-1-AP) and alpha tubulin (green, Cat. CL488-66031). The alpha tubulin was conjugated to CoraLite-488 fluorescent dye. In red, you can see the epithelial layer of tissue clearly stained, particularly the skin, eyes, and respiratory system. Nerves and neurons are stained green since they are rich in tubulin. Image credit: @Immunofluorescence on Instagram

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  FC experiment of HeLa using CL488-66031

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Alpha Tubulin (CL488-66031, Clone:1E4C11) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells, HEK-293 cells, HSC-T6 cells, NIH/3T3 cells
• Positive IF/ICC detected in: HeLa cells, mouse embryo tissue
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:300-1:600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 4 publications below

Product Information

CL488-66031 targets Alpha Tubulin in WB, IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, canine samples.

• Tested Reactivity: human, mouse, rat, canine
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Alpha Tubulin fusion protein Ag18034
• Full Name: tubulin, alpha 1b
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC009314
• Gene Symbol: Alpha Tubulin
• Gene ID (NCBI): 10376
• RRID: AB_2883232
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha tubulin is useful for scientists across fields as an internal control due to its high, ubiquitous expression pattern. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs. This anitbody is CL488(Ex/Em 488 nm/515 nm) conjugated.

Publications

Species	Application	Title
	IF	Free Radic Biol Med; Iron overload modulates follicular microenvironment via ROS/HIF-1α/FSHR signaling; Authors - Yaoqiu Wu
		Clin Genet; A novel homozygous CFAP65 mutation in humans causes male infertility with multiple morphological abnormalities of the sperm flagella.; Authors - Xueguang Zhang
human	IF	Transl Androl Urol; Novel bi-allelic mutations in DNAH1 cause multiple morphological abnormalities of the sperm flagella resulting in male infertility.; Authors - Chuan Jiang
mouse	IF	Reprod Toxicol; Bisphenol M inhibits mouse oocyte maturation in vitro by disrupting cytoskeleton architecture and cell cycle processes; Authors - Huilei Chen
mouse	IF	Am J Physiol Cell Physiol; Melatonin partially rescues defects induced by tranexamic acid exposure during oocyte maturation in mice; Authors - Fei Chen

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C