CoraLite® Plus 488-conjugated Alpha Tubulin Monoclonal antibody
Alpha Tubulin Monoclonal Antibody for WB, IF/ICC, FC (Intra)
Host / Isotype
Mouse / IgG2b
Reactivity
human, mouse, rat, canine
Applications
WB, IF/ICC, FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1E4C11
Cat no : CL488-66031
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HepG2 cells, HeLa cells, HEK-293 cells, HSC-T6 cells, NIH/3T3 cells |
Positive IF/ICC detected in | HeLa cells, mouse embryo tissue |
Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:300-1:600 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
IF | See 3 publications below |
Product Information
CL488-66031 targets Alpha Tubulin in WB, IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, canine samples.
Tested Reactivity | human, mouse, rat, canine |
Cited Reactivity | human, mouse |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | Alpha Tubulin fusion protein Ag18034 |
Full Name | tubulin, alpha 1b |
Calculated Molecular Weight | 50 kDa |
Observed Molecular Weight | 50-55 kDa |
GenBank Accession Number | BC009314 |
Gene Symbol | Alpha Tubulin |
Gene ID (NCBI) | 10376 |
RRID | AB_2883232 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha tubulin is useful for scientists across fields as an internal control due to its high, ubiquitous expression pattern. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs. This anitbody is CL488(Ex/Em 488 nm/515 nm) conjugated.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for CL Plus 488 Alpha Tubulin antibody CL488-66031 | Download protocol |
IF protocol for CL Plus 488 Alpha Tubulin antibody CL488-66031 | Download protocol |
FC protocol for CL Plus 488 Alpha Tubulin antibody CL488-66031 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Free Radic Biol Med Iron overload modulates follicular microenvironment via ROS/HIF-1α/FSHR signaling | ||
Clin Genet A novel homozygous CFAP65 mutation in humans causes male infertility with multiple morphological abnormalities of the sperm flagella. | ||
Transl Androl Urol Novel bi-allelic mutations in DNAH1 cause multiple morphological abnormalities of the sperm flagella resulting in male infertility. | ||
Reprod Toxicol Bisphenol M inhibits mouse oocyte maturation in vitro by disrupting cytoskeleton architecture and cell cycle processes |