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  • KD/KO Validated

HRP-conjugated Alpha Tubulin Monoclonal antibody

Alpha Tubulin Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, CoIP

Conjugate

HRP

CloneNo.

1E4C11

Cat no : HRP-66031

Synonyms

Alpha Tubulin, Alpha tubulin ubiquitous, alpha tubulin,a tubulin, HRP Tubulin Alpha, K ALPHA 1, TUBA1B, tubulin, Tubulin Alpha, Tubulin alpha 1B chain, Tubulin alpha ubiquitous chain, Tubulin K alpha 1, tubulin, alpha 1b, α-tubulin



Tested Applications

Positive WB detected inHeLa cells, Recombinant protein

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

HRP-66031 targets Alpha Tubulin in WB, CoIP applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Recombinant Protein
Full Name tubulin, alpha 1b
Calculated Molecular Weight 50 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC009314
Gene Symbol Alpha Tubulin
Gene ID (NCBI) 10376
RRIDAB_2687491
Conjugate HRP
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

What is the function of alpha-tubulin?

Alpha-tubulin belongs to a large superfamily of tubulin proteins. There are a number of different subtypes that have a molecular weight of ~50kDa and are able to bind to beta-tubulin, forming a heterodimer that polymerizes to microtubules as part of the cytoskeleton. These maintain cell structure, provide platforms for intracellular transport, and are also involved in cell division.

 

Where is alpha-tubulin expressed?

Alpha-tubulin is highly conserved and is present in nearly all eukaryotic cells as one of the building blocks of microtubules. The ubiquitous nature of this protein has led to its common use as a control protein for many tissue types as well as highlighting the structure of the cytoskeleton.

 

What are the post-translational modifications of alpha-tubulin?

The function and properties of microtubules are drastically affected by the post-translational modifications undergone by tubulin, which may occur to the tubulin dimer directly or to the polymerized microtubule. For example, the first modification to be identified was detyrosination,1 as most alpha-tubulins have a tyrosine at their terminus. This process affects microtubules more than dimers and leads to patches of detyrosination along the structure, regulating protein interactions and allowing subcellular compartments to be defined.2,3 Polyglutamylation also occurs at several sites within the carboxy-terminal tails. However, to date, the most-studied alpha tubulin modification is related to acetylation of lysine 40 (K40).

 

  1. Gundersen, G. G., Khawaja, S. &  Bulinski, J. C. Postpolymerization detyrosination of alpha-tubulin: a  mechanism for subcellular differentiation of microtubules. J. Cell  Biol. 105, 251-64 (1987).

  2. Galjart, N. Plus-End-Tracking Proteins  and Their Interactions at Microtubule Ends. Curr. Biol. 20,  R528-R537 (2010).

  3. Jiang, K. & Akhmanova, A.  Microtubule tip-interacting proteins: a view from both ends. Curr.  Opin. Cell Biol. 23, 94-101 (2011).


Publications

SpeciesApplicationTitle
humanWB

Cell

Human T-bet Governs Innate and Innate-like Adaptive IFN-γ Immunity against Mycobacteria.

Authors - Rui Yang
humanWB

Mol Cancer

Cell surface CD55 traffics to the nucleus leading to cisplatin resistance and stemness by inducing PRC2 and H3K27 trimethylation on chromatin in ovarian cancer

Authors - Rashmi Bharti
humanWB

Cell

Immune Checkpoint Inhibition Overcomes ADCP-Induced Immunosuppression by Macrophages.

Authors - Shicheng Su
ratWB

Cell Stem Cell

A small-molecule cocktail promotes mammalian cardiomyocyte proliferation and heart regeneration.

Authors - Jianyong Du
humanWB

Immunity

Human Virus-Derived Small RNAs Can Confer Antiviral Immunity in Mammals.

Authors - Yang Qiu
mouseWB

Immunity

Very-low-density lipoprotein receptor-enhanced lipid metabolism in pancreatic stellate cells promotes pancreatic fibrosis.

Authors - Xuguang Yang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Sara (Verified Customer) (10-05-2024)

I love Proteintech HRP-conjugated antibodies. These are def my favourites for WB controls (housekeeping proteins), I have got actin, tubulin and GAPDH. Very good bands, super reliable.

  • Applications: Western Blot
  • Cell Tissue Type: Mouse