Biotin-conjugated Alpha Tubulin Monoclonal antibody

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• KD/KO Validated

Alpha Tubulin Monoclonal Antibody for IHC

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, Canine

Applications

WB, IHC

Conjugate

Biotin

Publications

1

CloneNo.

1E4C11

Cat no : Biotin-66031

Synonyms

Alpha Tubulin, Alpha tubulin ubiquitous, alpha tubulin,a tubulin, K ALPHA 1, TUBA1B, Tubulin alpha 1B chain, Tubulin alpha ubiquitous chain, Tubulin K alpha 1, tubulin, alpha 1b, α-tubulin

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Validation Data Gallery

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  IHC staining of human liver cancer using Biotin-66031

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using Biotin-66031 (alpha Tubulin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using Biotin-66031

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using Biotin-66031 (alpha Tubulin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using Biotin-66031

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using Biotin-66031 (alpha Tubulin antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

The immunogen of Biotin-66031 is Alpha Tubulin Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat, Canine
• Cited Reactivity: mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Alpha Tubulin fusion protein Ag18034
• Full Name: tubulin, alpha 1b
• Calculated Molecular Weight: 50 kDa
• GenBank Accession Number: BC009314
• Gene Symbol: Alpha Tubulin
• Gene ID (NCBI): 10376
• RRID: AB_2883068
• Conjugate: Biotin
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

What is the function of alpha tubulin?

Alpha-tubulin belongs to a large superfamily of tubulin proteins. There are a number of different subtypes that have a molecular weight of ~50kDa and are able to bind to beta-tubulin, forming a heterodimer that polymerises to microtubules as part of the cytoskeleton. These maintain cell structure, provide platforms for intracellular transport and are also involved in cell division.

 Where is alpha-tubulin expressed?

Alpha tubulin is highly conserved and is present in nearly all eukaryotic cells as one of the building blocks of microtubules. The ubiquitous nature of this protein has led to its common use as a control protein for many tissue types as well as highlighting the structure of the cytoskeleton. 

What are the post-translational modifications of alpha tubulin?

The function and properties of microtubules are drastically affected by the post-translational modifications undergone by tubulin, which may occur to the tubulin dimer directly or to the polymerised mictotubule. For example, the first modification to be identified was detyrosination¹, as most alpha-tubulins have a tyrosine at their terminus. This process affects microtubules more than dimers and leads to patches of detyronisation along the structure, regulating protein interactions and allowing subcellular compartments to be defined.^2,3  Polyglutamylation also occurs on several sites within the carboxy-terminal tails. However, to date, the most-studied alpha tubulin modification is related to acetylation of lysine 40 (K40).

 1. Gundersen, G. G., Khawaja, S. & Bulinski, J. C. Postpolymerization detyrosination of alpha-tubulin: a mechanism for subcellular differentiation of microtubules. J. Cell Biol. 105, 251-64 (1987).

2. Galjart, N. Plus-End-Tracking Proteins and Their Interactions at Microtubule Ends. Curr. Biol. 20, R528-R537 (2010).

3. Jiang, K. & Akhmanova, A. Microtubule tip-interacting proteins: a view from both ends. Curr. Opin. Cell Biol. 23, 94-101 (2011).

Publications

Species	Application	Title
mouse	WB	Biochem Pharmacol; Bim transfer between Bcl-2-like protein and Hsp70 underlines Bcl-2/Hsp70 crosstalk to regulate apoptosis.; Authors - Hong Zhang