Alpha Tubulin Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

Alpha Tubulin Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, canine

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

1E4C11

Cat no : 66031-1-PBS

Synonyms

TUBA1B, Tubulin alpha 1B chain, Tubulin alpha, EC:3.6.5.-, Detyrosinated tubulin alpha-1B chain

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Validation Data Gallery

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  WB analysis using 66031-1-Ig (same clone as 66031-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66031-1-Ig (Alpha Tubulin antibody) at dilution of 1:200000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66031-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66031-1-Ig (same clone as 66031-1-PBS)

  WB result of Alpha Tubulin antibody (66031-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Alpha Tubulin transfected HeLa cells. This data was developed using the same antibody clone with 66031-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 66031-1-Ig (same clone as 66031-1-PBS)

  IP result of anti-Alpha Tubulin (IP:66031-1-Ig, 5ug; Detection:11224-1-AP 1:1000) with HeLa cells lysate 2800ug. This data was developed using the same antibody clone with 66031-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66031-1-Ig (same clone as 66031-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66031-1-Ig (alpha Tubulin antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66031-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66031-1-Ig (same clone as 66031-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66031-1-Ig (Alpha Tubulin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66031-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66031-1-Ig (same clone as 66031-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66031-1-Ig (Alpha Tubulin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66031-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66031-1-Ig (same clone as 66031-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66031-1-Ig (alpha Tubulin antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66031-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66031-1-Ig (same clone as 66031-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 66031-1-Ig(alpha Tubulin antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 66031-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66031-1-Ig (same clone as 66031-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed HepG2 cells using 66031-1-Ig(alpha Tubulin antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66031-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66031-1-Ig (same clone as 66031-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11 ) at dilution of 1:1000 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), Histone H1.2 antibody (19649-1-AP, green). This data was developed using the same antibody clone with 66031-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 66031-1-Ig (same clone as 66031-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11 ) at dilution of 1:1000 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), ZO-1 antibody (21773-1-AP, green). This data was developed using the same antibody clone with 66031-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 66031-1-Ig (same clone as 66031-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), ZO-1 antibody (21773-1-AP, red). This data was developed using the same antibody clone with 66031-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66031-1-Ig (same clone as 66031-1-PBS)

  Immunofluorescent analysis of HepG2 cells using 66031-1-Ig (alpha Tubulin antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L). This data was developed using the same antibody clone with 66031-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 66031-1-Ig (same clone as 66031-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Alpha Tubulin (66031-1-Ig, Clone:1E4C11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66031-1-PBS in a different storage buffer formulation.

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Published Applications

• WB: See 1 publications below

Product Information

66031-1-PBS targets Alpha Tubulin in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.

• Tested Reactivity: human, mouse, rat, canine
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Alpha Tubulin fusion protein Ag18034
• Full Name: tubulin, alpha 1b
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC009314
• Gene Symbol: Alpha Tubulin
• Gene ID (NCBI): 10376
• RRID: AB_11042766
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

What is the function of alpha tubulin?

Alpha-tubulin belongs to a large superfamily of tubulin proteins. There are a number of different subtypes that have a molecular weight of ~50kDa and are able to bind to beta-tubulin, forming a heterodimer that polymerises to microtubules as part of the cytoskeleton. These maintain cell structure, provide platforms for intracellular transport and are also involved in cell division.

 Where is alpha-tubulin expressed?

Alpha tubulin is highly conserved and is present in nearly all eukaryotic cells as one of the building blocks of microtubules. The ubiquitous nature of this protein has led to its common use as a control protein for many tissue types as well as highlighting the structure of the cytoskeleton. 

What are the post-translational modifications of alpha tubulin?

The function and properties of microtubules are drastically affected by the post-translational modifications undergone by tubulin, which may occur to the tubulin dimer directly or to the polymerised mictotubule. For example, the first modification to be identified was detyrosination¹, as most alpha-tubulins have a tyrosine at their terminus. This process affects microtubules more than dimers and leads to patches of detyronisation along the structure, regulating protein interactions and allowing subcellular compartments to be defined.^2,3  Polyglutamylation also occurs on several sites within the carboxy-terminal tails. However, to date, the most-studied alpha tubulin modification is related to acetylation of lysine 40 (K40).

 1. Gundersen, G. G., Khawaja, S. & Bulinski, J. C. Postpolymerization detyrosination of alpha-tubulin: a mechanism for subcellular differentiation of microtubules. J. Cell Biol. 105, 251-64 (1987).

2. Galjart, N. Plus-End-Tracking Proteins and Their Interactions at Microtubule Ends. Curr. Biol. 20, R528-R537 (2010).

3. Jiang, K. & Akhmanova, A. Microtubule tip-interacting proteins: a view from both ends. Curr. Opin. Cell Biol. 23, 94-101 (2011).

Publications

Species	Application	Title
	WB	Front Pharmacol; Liquiritin ameliorates painful diabetic neuropathy in SD rats by inhibiting NLRP3-MMP-9-mediated reversal of aquaporin-4 polarity in the glymphatic system; Authors - Shuai-Ying Jia