CoraLite® Plus 647-conjugated smooth muscle actin specific Monoclonal antibody

smooth muscle actin specific Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig and More (1)

Applications

IF, FC (Intra), FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Publications

3

CloneNo.

1E9A11

Cat no : CL647-67735

Synonyms

a SMA, AAT6, ACTA2, ACTA2 Smooth muscle Actin, Actin, aortic smooth muscle, ACTSA, ACTVS, Alpha actin 2, Alpha SMA, a-SMA, smooth muscle actin, smooth muscle actin specific

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Validation Data Gallery

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  IF Staining of mouse heart using CL647-67735

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite® Plus 647 smooth muscle actin specific antibody (CL647-67735, Clone: 1E9A11 ) at dilution of 1:100, CoraLite®488 Cardiac Troponin I antibody (CL488-66376, Clone: 1D5D6, green), CoraLite®594 N-cadherin antibody (CL594-22018, red). DAPI (blue).

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  IF Staining of mouse heart using CL647-67735

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite® Plus 647 smooth muscle actin specific antibody (CL647-67735, Clone: 1E9A11 ) at dilution of 1:200.

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  FC experiment of C2C12 using CL647-67735

  1X10^6 C2C12 cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Anti-Human smooth muscle actin specific (CL647-67735, Clone:1E9A11) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF detected in: mouse heart tissue
• Positive FC detected in: C2C12 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 2 publications below
• FC: See 1 publications below

Product Information

CL647-67735 targets smooth muscle actin specific in IF, FC (Intra), FC applications and shows reactivity with Human, mouse, rat, pig samples.

• Tested Reactivity: Human, mouse, rat, pig
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: actin, alpha 2, smooth muscle, aorta
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: NM_001613
• Gene Symbol: ACTA2
• Gene ID (NCBI): 59
• RRID: AB_2920302
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells.

Publications

Species	Application	Title
human	IF	J Med Virol; Molecular and immune signatures, and pathological trajectories of fatal COVID-19 lungs defined by in situ spatial single-cell transcriptome analysis; Authors - Arun Das
human	IF	Lab Invest; HSPB6 deficiency promotes the development of aortic dissection and rupture; Authors - Shiqi Gao
mouse	FC	Nat Commun; FAM3A reshapes VSMC fate specification in abdominal aortic aneurysm by regulating KLF4 ubiquitination; Authors - Chuxiang Lei

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C