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CoraLite®594-conjugated smooth muscle actin specific Monoclonal antibody

smooth muscle actin specific Monoclonal Antibody for IF-P, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

IF-P, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1E9A11

Cat no : CL594-67735

Synonyms

ACTA2, Alpha SMA, a-SMA, smooth muscle actin, actin

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using CoraLite®594-conjugated smooth muscle actin specif (CL594-67735)

    IF Staining of mouse heart using CL594-67735

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite®594 smooth muscle actin specific antibody (CL594-67735, Clone: 1E9A11 ) at dilution of 1:100.

  • Flow cytometry (FC) experiment of C2C12 cells using CoraLite®594-conjugated smooth muscle actin specif (CL594-67735)

    FC experiment of C2C12 using CL594-67735

    1X10^6 C2C12 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human smooth muscle actin specific (CL594-67735, Clone:1E9A11) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL594-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF-P detected inmouse heart tissue
Positive FC (Intra) detected inC2C12 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67735 targets smooth muscle actin specific in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name actin, alpha 2, smooth muscle, aorta
Calculated Molecular Weight 42 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberNM_001613
Gene Symbol Alpha smooth muscle actin
Gene ID (NCBI) 59
RRIDAB_2920177
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells.

Protocols

Product Specific Protocols
IF protocol for CL594 smooth muscle actin specific antibody CL594-67735Download protocol
FC protocol for CL594 smooth muscle actin specific antibody CL594-67735Download protocol
Standard Protocols
Click here to view our Standard Protocols