Tested Applications
| Positive WB detected in | rat stomach tissue, rabbit stomach tissue, pig vein tissue, pig stomach tissue, rabbit large intestine tissue, pig colon tissue, rat colon tissue, mouse colon tissue |
| Positive IHC detected in | human colon tissue, human appendicitis tissue, human liver tissue, human liver cancer tissue, rat colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | rat heart tissue |
| Positive IF-Fro detected in | mouse breast cancer |
| Positive IF/ICC detected in | C2C12 cells |
| Positive FC (Intra) detected in | C2C12 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 |
| Immunohistochemistry (IHC) | IHC : 1:2500-1:10000 |
| Immunofluorescence (IF)-P | IF-P : 1:1000-1:4000 |
| Immunofluorescence (IF)-FRO | IF-FRO : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67735-1-Ig targets Alpha smooth muscle actin specific in WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.
| Tested Reactivity | human, mouse, rat, pig, rabbit |
| Cited Reactivity | human, mouse, rat, pig, rabbit, zebrafish |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | actin, alpha 2, smooth muscle, aorta |
| Calculated Molecular Weight | 42 kDa |
| Observed Molecular Weight | 42 kDa |
| GenBank Accession Number | NM_001613 |
| Gene Symbol | Alpha smooth muscle actin |
| Gene ID (NCBI) | 59 |
| RRID | AB_2918504 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P62736 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for Alpha smooth muscle actin specific antibody 67735-1-Ig | Download protocol |
| IF protocol for Alpha smooth muscle actin specific antibody 67735-1-Ig | Download protocol |
| IHC protocol for Alpha smooth muscle actin specific antibody 67735-1-Ig | Download protocol |
| WB protocol for Alpha smooth muscle actin specific antibody 67735-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-SPARC antibody (Cat# 67735-1-Ig) has accumulated 421 citations published in high-impact journals including Nature Communications, Advanced Materials, ACS Nano, Autophagy, Hepatology, Journal of Nanobiotechnology, Acta Biomaterialia, Cell Reports, Phytomedicine, and Journal of Translational Medicine. Key research fields include fibrosis (pulmonary, renal, hepatic, cardiac), wound healing, cardiovascular diseases (atherosclerosis, myocardial infarction, aortic aneurysm), cancer biology, ferroptosis and pyroptosis, tissue engineering, vascular remodeling, and immunology.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Overexpression of STX11 alleviates pulmonary fibrosis by inhibiting fibroblast activation via the PI3K/AKT/mTOR pathway | ||
Mol Cancer circFARP1 enables cancer-associated fibroblasts to promote gemcitabine resistance in pancreatic cancer via the LIF/STAT3 axis. | ||
Adv Mater A "Bridge-Building" Glycan Scaffold Mimicking Microbial Invasion for In Situ Endothelialization. | ||
Adv Mater Photothermal and Photocatalytic Glycol Chitosan and Polydopamine-Grafted Oxygen Vacancy Bismuth Oxyiodide (BiO1-x I) Nanoparticles for the Diagnosis and Targeted Therapy of Diabetic Wounds | ||
Adv Mater In Situ Triggered Self-Contraction Bioactive Microgel Assembly Accelerates Diabetic Skin Wound Healing by Activating Mechanotransduction and Biochemical Pathway | ||
Cancer Commun (Lond) Targeting SPHK1 in macrophages remodels the tumor microenvironment and enhances anti-PD-1 immunotherapy efficacy in colorectal cancer liver metastasis. |
Reviews
What customers say
All three reviews rate this antibody 5 stars. Users report successful performance across multiple applications: immunohistochemistry on FFPE mouse organs (liver, kidney, spleen, heart, lungs) and free-floating brains using Tris-EDTA antigen retrieval at 1:500; immunofluorescence via confocal microscopy at 1:200; and Western blot on mouse liver tissue at 1:20000 in 1X TBST. Overall, customers find it reliable and effective for detecting smooth muscle actin in various formats.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Mary (Verified Customer) (02-02-2026) | FFPE organs (liver, kidney, spleen, heart, lungs) work well with Tris-EDTA HIAR. Fluorescent immunohistochemistry.
|
Sai Sindhura (Verified Customer) (01-08-2026) | smooth muscle actin antibody showed good through confocal
|
Kamal (Verified Customer) (10-16-2025) | Antibody works when diluted in 1X TBST.
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