Alpha smooth muscle actin specific Monoclonal antibody

Alpha smooth muscle actin specific Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit and More (1)

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

129

CloneNo.

1E9A11

Cat no : 67735-1-Ig

Synonyms

ACTA2, Alpha SMA, a-SMA, smooth muscle actin, Actin, aortic smooth muscle, intermediate form

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Validation Data Gallery

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  WB analysis using 67735-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67735-1-Ig (a-SMA specific antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67735-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67735-1-Ig (smooth muscle actin specific antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67735-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67735-1-Ig (a-SMA specific antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig vein using 67735-1-Ig

  pig vein tissue were subjected to SDS PAGE followed by western blot with 67735-1-Ig (a-SMA specific antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 67735-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67735-1-Ig (a-SMA specific antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat colon using 67735-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 67735-1-Ig (a-SMA specific antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 67735-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67735-1-Ig (smooth muscle actin specific antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human appendicitis using 67735-1-Ig

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 67735-1-Ig (smooth muscle actin specific antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67735-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67735-1-Ig (smooth muscle actin specific antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 67735-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67735-1-Ig (smooth muscle actin specific antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of rat heart using 67735-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed rat heart tissue using a-SMA specific antibody (67735-1-Ig, Clone: 1E9A11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of C2C12 using 67735-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using smooth muscle actin specific antibody (67735-1-Ig, Clone: 1E9A11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of C2C12 using 67735-1-Ig

  1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human smooth muscle actin specific (67735-1-Ig, Clone:1E9A11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: rat stomach tissue, rabbit stomach tissue, pig vein tissue, pig stomach tissue, rabbit large intestine tissue, pig colon tissue, rat colon tissue, mouse colon tissue
• Positive IHC detected in: human colon tissue, human appendicitis tissue, human liver tissue, human liver cancer tissue, rat colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: rat heart tissue
• Positive IF/ICC detected in: C2C12 cells
• Positive FC (Intra) detected in: C2C12 cells

Recommended dilution

• Western Blot (WB): WB : 1:20000-1:100000
• Immunohistochemistry (IHC): IHC : 1:2500-1:10000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 61 publications below
• IHC: See 32 publications below
• IF: See 79 publications below

Product Information

67735-1-Ig targets Alpha smooth muscle actin specific in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: human, mouse, rat, pig, rabbit
• Cited Reactivity: human, mouse, rat, pig, zebrafish
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: actin, alpha 2, smooth muscle, aorta
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: NM_001613
• Gene Symbol: Alpha smooth muscle actin
• Gene ID (NCBI): 59
• RRID: AB_2918504
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells.

Publications

Species	Application	Title
mouse	IF	Adv Mater; A "Bridge-Building" Glycan Scaffold Mimicking Microbial Invasion for In Situ Endothelialization.; Authors - Ruoyu Mu
mouse	IF	Nat Commun; ALKBH5-mediated m6A modification of IL-11 drives macrophage-to-myofibroblast transition and pathological cardiac fibrosis in mice; Authors - Tao Zhuang
human	IHC	Mol Cancer; circFARP1 enables cancer-associated fibroblasts to promote gemcitabine resistance in pancreatic cancer via the LIF/STAT3 axis.; Authors - Chonghui Hu
human	IF	Adv Sci (Weinh); Single-Cell Transcriptomic Analysis of Primary and Metastatic Tumor Ecosystems in Esophageal Squamous Cell Carcinoma; Authors - Yongxu Jia
mouse	IF	Acta Pharm Sin B; ADAR1 regulates vascular remodeling in hypoxic pulmonary hypertension through N1-methyladenosine modification of circCDK17; Authors - Junting Zhang
rat	WB	Biomaterials; Extended-release of therapeutic microRNA via a host-guest supramolecular hydrogel to locally alleviate renal interstitial fibrosis.; Authors - Yingying Xu

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C