CoraLite®594-conjugated SOX2 Monoclonal antibody

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• KD/KO Validated

SOX2 Monoclonal Antibody for IF/ICC, IF-P, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, IF-P, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

4B9B10

Cat no : CL594-66411

Synonyms

Transcription factor SOX 2, SOX 2, MCOPS3, MCOPS 3, ANOP3

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Validation Data Gallery

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  IF Staining of mouse brain using CL594-66411

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CL594-66411 (SOX2 antibody) at dilution of 1:50.

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  IF Staining of rat brain using CL594-66411

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using CoraLite®594 SOX2 antibody (CL594-66411, Clone: 4B9B10 ) at dilution of 1:400.

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  IF Staining of mouse brain using CL594-66411

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 SOX2 antibody (CL594-66411, Clone: 4B9B10 ) at dilution of 1:200. DAPI (blue).

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  IF Staining of U-251 using CL594-66411

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using CoraLite®594 SOX2 antibody (CL594-66411, Clone: 4B9B10 ) at dilution of 1:200.

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  FC experiment of U-251 using CL594-66411

  1X10^6 U-251 cells were intracellularly stained with 0.2 ug CoraLite®594 Anti-Human SOX2 (CL594-66411, Clone:4B9B10) (red), or 0.2 ug Mouse IgG1 Isotype Control (CL594-66360, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive IF-P detected in: mouse brain tissue, rat brain tissue
• Positive IF/ICC detected in: U-251 cells
• Positive FC (Intra) detected in: U-251 cells

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66411 targets SOX2 in IF/ICC, IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SOX2 fusion protein Ag13635
• Full Name: SRY (sex determining region Y)-box 2
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 34-40 kDa
• GenBank Accession Number: BC013923
• Gene Symbol: SOX2
• Gene ID (NCBI): 6657
• RRID: AB_2883578
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Sox2, also known as SRY (sex determining region Y)-box 2, is a transcription factor essential for maintaining self-renewal of undifferentiated ES cells and is one of the key transcription factors used to reprogram mouse and human fibroblasts to a pluripotent state. Sox2 expressed in undifferentiated pluripotent stem cells and germ cells during development. This antibody is raised against an internal region of human SOX2. And the antibody is conjugated with CL594, Ex/Em 593 nm/614 nm.

Related Products

Foxp3 / Transcription Factor Staining Buffer Kit

PF00011