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CoraLite®594-conjugated Mouse IgG1 isotype control Monoclonal antibody

Mouse IgG1 isotype control Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1

Reactivity

Megathura crenulata

Applications

FC

Conjugate

CoraLite® Plus 594 Fluorescent Dye

CloneNo.

1F8D3

Cat no : CL594-66360

Synonyms

Kappa light chain, KLH, Mouse IgG1 isotype control

Validation Data Gallery

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  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite® Plus 594-conjugated Mouse IgG1 isotype c (CL594-66360)

    FC experiment of HepG2 using CL594-66360

    1X10^6 HepG2 cells were stained with 0.20 ug CoraLite® Plus 594-conjugated mouse IgG1 isotype control (CL594-66360, Clone:1F8D3) (red) or 0.20 ug CoraLite® Plus 594-conjugated Vimentin antibody (CL594-60330) (green), or not stained (blank) (blue). Cells were fixed with 90% MeOH.

Tested Applications

Positive FC detected in

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66360 targets Mouse IgG1 isotype control in FC applications and shows reactivity with Megathura crenulata samples.

Tested Reactivity Megathura crenulata
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name Mouse IgG1 isotype control
Gene Symbol
Gene ID (NCBI)
RRIDAB_2883567
Conjugate CoraLite® Plus 594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

 This antibody is used as the isotype control of mouse IgG1 (Kappa). It can be used as the isotype control in Flow Cytometry and other experiments. The immunogen of this antibody is Keyhole Limpet Hemocyanin (KLH).

0.05-0.2 ug per test is suggested for FC experiment.

Protocols

Product Specific Protocols
FC protocol for CL Plus 594 Mouse IgG1 isotype control antibody CL594-66360Download protocol
Standard Protocols
Click here to view our Standard Protocols