CoraLite® Plus 488-conjugated SOX2 Monoclonal antibody

SOX2 Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, mouse

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

4B9B10

Cat no : CL488-66411

Synonyms

ANOP3, MCOPS3, SOX2, Transcription factor SOX 2

---

Validation Data Gallery

• 

  IF Staining of mouse brain using CL488-66411

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CL488-66411 (SOX2 antibody) at dilution of 1:50.

---

Tested Applications

• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66411 targets SOX2 in IF-P applications and shows reactivity with human, pig, mouse samples.

• Tested Reactivity: human, pig, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SOX2 fusion protein Ag13635
• Full Name: SRY (sex determining region Y)-box 2
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 34-40 kDa
• GenBank Accession Number: BC013923
• Gene Symbol: SOX2
• Gene ID (NCBI): 6657
• RRID: AB_2883330
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Sox2, also known as SRY (sex determining region Y)-box 2, is a transcription factor essential for maintaining self-renewal of undifferentiated ES cells and is one of the key transcription factors used to reprogram mouse and human fibroblasts to a pluripotent state. Sox2 expressed in undifferentiated pluripotent stem cells and germ cells during development.. This antibody is raised against an internal region of human SOX2. And the antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.