SMURF2 Recombinant antibody

SMURF2 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 84445-1-RR
Clone No.241486F3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

SMAD-specific E3 ubiquitin-protein ligase 2, hSMURF2, HECT-type E3 ubiquitin transferase SMURF2, EC:2.3.2.26, E3 ubiquitin-protein ligase SMURF2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inU2OS cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84445-1-RR targets SMURF2 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SMURF2 fusion protein Ag33419 Predict reactive species
Full Name SMAD specific E3 ubiquitin protein ligase 2
Calculated Molecular Weight 748 aa, 86 kDa
GenBank Accession NumberBC093876
Gene Symbol SMURF2
Gene ID (NCBI) 64750
RRIDAB_3671970
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9HAU4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SMURF2 is a HECT domain E3 ubiquitin ligase involved in degradation of SMADs,TGF-beta receptor,and other substrates.It also functions in regulation of neuronal and planar cell polarity, induction of senescence, and tumor suppression(PMID:22231558).SMURF2 associates constitutively with SMAD7 and it is nuclear, but binding to SMAD7 induces export and recruitment to activated TGFBR, where it causes degradation of receptors and of SMAD7 via proteasomal and lysosomal pathways(PMIF:11163210).

Protocols

Product Specific Protocols
IF protocol for SMURF2 antibody 84445-1-RRDownload protocol
FC protocol for SMURF2 antibody 84445-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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