CoraLite® Plus 488-conjugated SMURF2 Recombinant antibody

SMURF2 Uni-rAb^TM Recombinant Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

241486F3

Cat no : CL488-84445

Synonyms

SMAD-specific E3 ubiquitin-protein ligase 2, hSMURF2, HECT-type E3 ubiquitin transferase SMURF2, EC:2.3.2.26, E3 ubiquitin-protein ligase SMURF2

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Validation Data Gallery

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  IF Staining of U2OS using CL488-84445

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CoraLite® Plus 488 SMURF2 antibody (CL488-84445, Clone: 241486F3 ) at dilution of 1:200, CL594-Phalloidin (red).

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Tested Applications

• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL488-84445 is SMURF2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SMURF2 fusion protein Ag33419
• Full Name: SMAD specific E3 ubiquitin protein ligase 2
• Calculated Molecular Weight: 748 aa, 86 kDa
• GenBank Accession Number: BC093876
• Gene Symbol: SMURF2
• Gene ID (NCBI): 64750
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SMURF2 is a HECT domain E3 ubiquitin ligase involved in degradation of SMADs,TGF-beta receptor,and other substrates.It also functions in regulation of neuronal and planar cell polarity, induction of senescence, and tumor suppression(PMID:22231558).SMURF2 associates constitutively with SMAD7 and it is nuclear, but binding to SMAD7 induces export and recruitment to activated TGFBR, where it causes degradation of receptors and of SMAD7 via proteasomal and lysosomal pathways(PMIF:11163210).