PE-conjugated NAT10 Monoclonal antibody

• Featured Product
• KD/KO Validated

NAT10 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

PE Fluorescent Dye

CloneNo.

3E3D8

Cat no : PE-67465

Synonyms

N acetyltransferase 10, KIAA1709, EC:2.3.1.-, ALP, 3E3D8

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Validation Data Gallery

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  FC experiment of NIH/3T3 using PE-67465

  1x10^6 NIH/3T3 cells were intracellularly stained with 0.4 ug PE Nat10 Monoclonal Antibody (PE-67465, Clone:3E3D8)(red), or 0.4 ug PE Mouse IgG2a Isotype Control (C1.18.4) (PE-65208, Clone: C1.18.4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive FC (Intra) detected in: NIH/3T3 cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

PE-67465 targets NAT10 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: NAT10 fusion protein Ag4355
• Full Name: N-acetyltransferase 10 (GCN5-related)
• Calculated Molecular Weight: 1025 aa, 116 kDa
• Observed Molecular Weight: 116 kDa
• GenBank Accession Number: BC035558
• Gene Symbol: NAT10
• Gene ID (NCBI): 55226
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.1% sodium azide and 0.5% BSA.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage.

Background Information

NAT10 (N-acetyltransferase 10) is a nucleolar protein that is involved in regulation of telomerase activity, DNA damage response, and cytokinesis. It also plays a role in maintaining nuclear shape. Inhibition of NAT10 has been reported to rescue the misshapen nuclei in laminopathic cells via microtubule reorganization.