NAT10 Monoclonal antibody

• Featured Product
• KD/KO Validated

NAT10 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3E3D8

Cat no : 67465-1-Ig

Synonyms

N acetyltransferase 10, KIAA1709, EC:2.3.1.-, ALP, 3E3D8

---

Validation Data Gallery

• 

  WB analysis using 67465-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67465-1-Ig (NAT10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 67465-1-Ig

  WB result of NAT10 antibody (67465-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NAT10 transfected A549 cells.

• 

  WB analysis of HeLa using 67465-1-Ig

  WB result of NAT10 antibody (67465-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NAT10 transfected HeLa cells.

---

Tested Applications

• Positive WB detected in: LNCaP cells, A549 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, Neuro-2a cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67465-1-Ig targets NAT10 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: NAT10 fusion protein Ag4355
• Full Name: N-acetyltransferase 10 (GCN5-related)
• Calculated Molecular Weight: 1025 aa, 116 kDa
• Observed Molecular Weight: 116 kDa
• GenBank Accession Number: BC035558
• Gene Symbol: NAT10
• Gene ID (NCBI): 55226
• RRID: AB_2882694
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NAT10 (N-acetyltransferase 10) is a nucleolar protein that is involved in regulation of telomerase activity, DNA damage response, and cytokinesis. It also plays a role in maintaining nuclear shape. Inhibition of NAT10 has been reported to rescue the misshapen nuclei in laminopathic cells via microtubule reorganization.