ChromoTek Myc-Trap® Magnetic Agarose, kit

The ChromoTek Myc-Trap® Magnetic Agarose is an affinity resin for IP of Myc-fusion proteins. It consists of a Myc Nanobody/VHH coupled to magnetic agarose beads.

Specificity

Myc-Tag

Applications

IP, CoIP, ChIP, RIP

Conjugate

Magnetic agarose

Type

Nanobody

Cat no : ytmak

Synonyms

Myc tag, Myc, Myc-trap, EQKLISEEDL



Product Information

The ChromoTek Myc-Trap® Magnetic Agarose is an affinity resin for IP of Myc-fusion proteins. It consists of a Myc Nanobody/VHH coupled to magnetic agarose beads.

DescriptionThe Myc-Trap Magnetic Agarose Kit contains Myc-Trap Magnetic Agarose, lysis, wash, and elution buffers for efficient immunoprecipitation of Myc-tagged proteins and their interacting factors.

• Fast, reliable & efficient one-step immunoprecipitation

• Ready-to-use

• No heavy & light antibody chains on the SDS-PAGE

• Stable under harsh washing conditions

• Suitable for downstream mass spec analysis

ApplicationsIP, CoIP, ChIP, RIP
Specificity/TargetMyc-tag sequence motif EQKLISEEDL at the N-terminus, C-terminus, or internal site of the fusion protein. Endogenous c-myc is NOT bound.
Binding capacity17.5 μg of recombinant Myc-tagged protein (~42 kDa) per 25 μL bead slurry
ConjugateMagnetic agarose beads; bead size: ~40 µm (cross-linked 6 % magnetic agarose beads)
Elution buffer2x Myc peptide.
SDS sample buffer
0.2 M glycine pH 2.5
Wash buffer compatibility2 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 1 % Triton X-100
Type Nanobody
ClassRecombinant
HostAlpaca
Affinity (KD) 1x Myc-tag: Dissociation constant KD of 500 nM
2x Myc-tag: Dissociation constant KD of 0.5 nM
Compatibility with mass spectrometryThe Myc-Trap® is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
RRIDAB_2631370
Storage Buffer20% ethanol
Storage ConditionShipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze!

Kit components

ComponentDescription
Myc-Trap® Magnetic Agarose20 reactions (500 µL)
Lysis bufferOptimized for cytoplasmatic proteins and mammalian cell lysis
RIPA bufferOptimized for nuclear/chromatin proteins and mammalian cell lysis
Wash buffer Removal of unwanted proteins, peptides, etc.
Dilution bufferDilution of cell lysate
Elution bufferFor acidic elution

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Mona (Verified Customer) (08-27-2022)

I could pull down a Myc tagged protein with this kit. The efficiency was 100%. Highly recommended.

  • Applications: Immunoprecipitation
  • Cell Tissue Type: HeLa cell