ChromoTek Myc-Trap® Agarose, kit

Myc-Trap® Agarose is an affinity reagent for IP and purification of Myc-tagged proteins. It consists of a Myc Nanobody/ VHH coupled to agarose beads.

Specificity

Myc-Tag

Applications

IP, CoIP, ChIP, RIP

Conjugate

Agarose

Type

Nanobody

Publications

7

Cat no : ytak

Synonyms

Myc tag, Myc, Myc-trap, EQKLISEEDL

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Validation Data Gallery

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  Myc-Trap Agarose for immunoprecipitation of Myc-tagged proteins.

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Product Information

Myc-Trap® Agarose is an affinity reagent for IP and purification of Myc-tagged proteins. It consists of a Myc Nanobody/ VHH coupled to agarose beads.

• Description: The Myc-Trap Agarose Kit contains Myc-Trap Agarose, lysis, wash, and elution buffers for efficient immunoprecipitation of Myc-tagged proteins and their interacting factors.

  • Fast, reliable & efficient one-step immunoprecipitation

  • Ready-to-use

  • No heavy & light antibody chains on the SDS-PAGE

  • Stable under harsh washing conditions

  • Elution with peptide

• Applications: IP, CoIP, ChIP, RIP
• Specificity/Target: Myc-tag sequence motif EQKLISEEDL at the N-terminus, C-terminus, or internal site of the fusion protein. Endogenous c-myc is NOT bound.
• Binding capacity: 17.5 μg of recombinant Myc-tagged protein (~42 kDa) per 25 μL bead slurry
• Conjugate: Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)
• Elution buffer: 2x Myc peptide.; SDS sample buffer; 0.2 M glycine pH 2.5
• Wash buffer compatibility: 2 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 1 % Triton X-100
• Type: Nanobody
• Class: Recombinant
• Host: Alpaca
• Affinity (KD): 1x Myc-tag: Dissociation constant K_D of 500 nM; 2x Myc-tag: Dissociation constant K_D of 0.5 nM
• Compatibility with mass spectrometry: The Myc-Trap® is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
• RRID: AB_2631369
• Storage Buffer: 20% ethanol
• Storage Condition: Shipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze!

Kit components

Component	Description
Myc-Trap® Agarose	20 reactions (500 µL)
Lysis buffer	Optimized for cytoplasmatic proteins and mammalian cell lysis
RIPA buffer	Optimized for nuclear/chromatin proteins and mammalian cell lysis
Wash buffer	Removal of unwanted proteins, peptides, etc.
Dilution buffer	Dilution of cell lysate
Elution buffer	For acidic elution

Other Formats

Myc-Trap Agarose

Myc-Trap Magnetic Agarose kit

Myc-Trap Magnetic Agarose

Publications

Application	Title
IP	Proc Natl Acad Sci U S A; Molecular determinants of homo- and heteromeric interactions of Junctophilin-1 at triads in adult skeletal muscle fibers.; Authors - Daniela Rossi
IP	PLoS Pathog; HEXIM1-Tat chimera inhibits HIV-1 replication.; Authors - Marie Leoz
IP	Int J Mol Sci; Yil102c-A is a Functional Homologue of the DPMII Subunit of Dolichyl Phosphate Mannose Synthase in Saccharomyces cerevisiae.; Authors - Sebastian Piłsyk
IP	J Mol Biol; RAN Translation of the Expanded CAG Repeats in the SCA3 Disease Context.; Authors - Magdalena Jazurek-Ciesiolka
	J Pharmacol Exp Ther; Inhibiting Sialidase-Induced TGF-β1 Activation Attenuates Pulmonary Fibrosis in Mice.; Authors - Tejas R Karhadkar
CoIP	PLoS One; The interplay of Rac1 activity, ubiquitination and GDI binding and its consequences for endothelial cell spreading.; Authors - Jisca Majolée