1X10^6 human PBMCs were surface stained with 0.2 ug CoraLite® Plus 750 Anti-Mouse IgG2b isotype control (CL750-66360-3, Clone:11B8C4) (red), or CoraLite® Plus 750 Anti-Human CD4 (OKT4) (green), or unstained (blue). Cells were not fixed. Lymphocytes were gated.
1X10^6 human PBMCs were surface stained with 0.2 ug CoraLite® Plus 750 Anti-Mouse IgG2b isotype control (CL750-66360-3, Clone:11B8C4) (red), or CoraLite® Plus 750 Anti-Human CD4 (OKT4) (green), or unstained (blue). Cells were not fixed. Lymphocytes were gated.
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This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
CL750-66360-3 targets IgG2b isotype control in FC applications and shows reactivity with Megathura crenulata samples.
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
Background Information
This antibody is used as the isotype control of mouse IgG2b. It can be used as the isotype control in Flow Cytometry, Immuno-Precipitation and other experiments. The immunogen of this antibody is Keyhole Limpet Hemocyanin (KLH).
Protocols
Product Specific Protocols
FC protocol for CL Plus 750 IgG2b isotype control antibody CL750-66360-3
1X10^6 human PBMCs were surface stained with 0.2 ug CoraLite® Plus 750 Anti-Mouse IgG2b isotype control (CL750-66360-3, Clone:11B8C4) (red), or CoraLite® Plus 750 Anti-Human CD4 (OKT4) (green), or unstained (blue). Cells were not fixed. Lymphocytes were gated.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
