CoraLite® Plus 488 Mouse IgG2b isotype control (11B8C4)

IgG2b isotype control Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2b

Reactivity

Megathura crenulata

Applications

FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

11B8C4

Cat no : CL488-66360-3

Synonyms

Mouse IgG2b, Mouse IgG2b isotype control

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Validation Data Gallery

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  FC experiment of HeLa using CL488-66360-3

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Mouse IgG2b isotype control (CL488-66360-3, Clone:11B8C4) (red), or 0.4 ug CoraLite® Plus 488-conjugated Beta Actin Monoclonal antibody (green) or unstained (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive FC (Intra) detected in
Positive FC detected in

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66360-3 targets IgG2b isotype control in FC applications and shows reactivity with Megathura crenulata samples.

• Tested Reactivity: Megathura crenulata
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: IgG2b isotype control
• RRID: AB_2883318
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.1% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

This antibody is used as the isotype control of mouse IgG2b. It can be used as the isotype control in Flow Cytometry and other experiments. The immunogen of this antibody is Keyhole Limpet Hemocyanin (KLH).

0.05-0.2 ug per test is suggested for FC experiment.