1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Mouse IgG2b isotype control (CL488-66360-3, Clone:11B8C4) (red), or 0.4 ug CoraLite® Plus 488-conjugated Beta Actin Monoclonal antibody (green) or unstained (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Mouse IgG2b isotype control (CL488-66360-3, Clone:11B8C4) (red), or 0.4 ug CoraLite® Plus 488-conjugated Beta Actin Monoclonal antibody (green) or unstained (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
CL488-66360-3 targets IgG2b isotype control in FC applications and shows reactivity with Megathura crenulata samples.
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
Background Information
This antibody is used as the isotype control of mouse IgG2b. It can be used as the isotype control in Flow Cytometry and other experiments. The immunogen of this antibody is Keyhole Limpet Hemocyanin (KLH).
0.05-0.2 ug per test is suggested for FC experiment.
Protocols
Product Specific Protocols
FC protocol for CL Plus 488 IgG2b isotype control antibody CL488-66360-3
1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Mouse IgG2b isotype control (CL488-66360-3, Clone:11B8C4) (red), or 0.4 ug CoraLite® Plus 488-conjugated Beta Actin Monoclonal antibody (green) or unstained (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
IgG2b isotype control Monoclonal antibody
Catalog Number
CL488-66360-3
Citations
-
Dilutions
FC (INTRA) :
Applications
FC
Reactivity
megathura crenulata
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
