CoraLite®555 Mouse IgG2b isotype control (11B8C4)

IgG2b isotype control Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2b

Reactivity

Megathura crenulata

Applications

FC

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

11B8C4

Cat no : CL555-66360-3

Synonyms

Mouse IgG2b, Mouse IgG2b isotype control

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Validation Data Gallery

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  FC experiment of human PBMCs using CL555-66360-3

  1X10^6 human PBMCs were surface stained with 0.2 ug CoraLite®555 Anti-Mouse IgG2b isotype control (CL555-66360-3, Clone:11B8C4) (red), or CoraLite®555 Anti-Human CD4 (OKT4) (green), or unstained (blue). Cells were not fixed. Lymphocytes were gated.

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Tested Applications

Positive FC detected in

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-66360-3 targets IgG2b isotype control in FC applications and shows reactivity with Megathura crenulata samples.

• Tested Reactivity: Megathura crenulata
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: IgG2b isotype control
• Conjugate: CoraLite®555 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 557 nm / 570 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

This antibody is used as the isotype control of mouse IgG2b. It can be used as the isotype control in Flow Cytometry, Immuno-Precipitation and other experiments. The immunogen of this antibody is Keyhole Limpet Hemocyanin (KLH).