Mouse IgG2b isotype control Monoclonal antibody

Mouse IgG2b isotype control Monoclonal Antibody for FC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Megathura crenulata

Applications

WB, FC, chIP, ELISA

Conjugate

Unconjugated

CloneNo.

11B8C4

Cat no : 66360-3-Ig

Synonyms

IgG2b isotype, mouse IgG control, Mouse IgG2b control, Mouse IgG2b isotype control


"Mouse IgG2b isotype control Antibodies" Comparison

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Applications:
  • WB analysis using 66360-3-Ig

    WB analysis using 66360-3-Ig

    Various lysates (50 ug/lane) were subjected to SDS PAGE followed by western blot with 66360-3-Ig (Mouse IgG2b isotype control antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • FC experiment of Jurkat using 66360-3-Ig

    FC experiment of Jurkat using 66360-3-Ig

    1X10^6 Jurkat cells were stained with 0.20ug Mouse IgG2b isotype control antibody (66360-3-Ig, red) or control antibody (blue). Fixed with 90% MeOH.


Tested Applications

Positive WB detected in
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Published Applications

ChIPSee 2 publications below

Product Information

66360-3-Ig targets Mouse IgG2b isotype control in WB, FC, chIP, ELISA applications and shows reactivity with Megathura crenulata samples.

Tested Reactivity Megathura crenulata
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Recombinant protein
Full Name Mouse IgG2b isotype control
Gene symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

This antibody is used as the isotype control of mouse IgG2b. It can be used as the isotype control in Flow Cytometry, Immuno-Precipitation and other experiments. The immunogen of this antibody is Keyhole Limpet Hemocyanin (KLH).

Protocols

Product Specific Protocols
WB protocol for Mouse IgG2b isotype control antibody 66360-3-IgDownload protocol
FC protocol for Mouse IgG2b isotype control antibody 66360-3-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
chIP

Cell Death Dis

E2F1 mediated DDX11 transcriptional activation promotes hepatocellular carcinoma progression through PI3K/AKT/mTOR pathway.

Authors - Yan Yu
ChIP

Front Oncol

The COX10-AS1/miR-641/E2F6 Feedback Loop Is Involved in the Progression of Glioma.

Authors - Liang Liu