Mouse IgG2b isotype control Monoclonal antibody

Mouse IgG2b isotype control Monoclonal Antibody for WB, IHC, FC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

megathura crenulata

Applications

WB, IHC, FC , chIP, ELISA

Conjugate

Unconjugated

Publications

5

CloneNo.

11B8C4

Cat no : 66360-3-Ig

Synonyms

Mouse IgG2b

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• Validation Data Gallery
• Proteintech vs. Other Vendors

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  WB analysis using 66360-3-Ig

  Various lysates (50 ug/lane) were subjected to SDS PAGE followed by western blot with 66360-3-Ig (Mouse IgG2b isotype control antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 66360-3-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66360-3-Ig (Mouse IgG2b isotype control antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 66360-3-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66360-3-Ig (Mouse IgG2b isotype control antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of human PBMCs using 66360-3-Ig

  1x10^6 human PBMCs were surface stained with 0.5 ug Mouse IgG2b isotype control Monoclonal antibody (66360-3-Ig, Clone: 11B8C4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1) (red), or 0.5 ug Anti-Human CD20 (65085-1-Ig) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1) (green), or unstained (blue). Cells were not fixed.

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Tested Applications

• Positive IHC detected in: Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: human PBMCs

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:1500-1:6000
• Flow Cytometry (FC): FC : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• ChIP: See 2 publications below

Product Information

66360-3-Ig targets Mouse IgG2b isotype control in WB, IHC, FC , chIP, ELISA applications and shows reactivity with megathura crenulata samples.

• Tested Reactivity: megathura crenulata
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: Mouse IgG2b isotype control
• RRID: AB_2881740
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

This antibody is used as the isotype control of mouse IgG2b. It can be used as the isotype control in Flow Cytometry, Immuno-Precipitation and other experiments. The immunogen of this antibody is Keyhole Limpet Hemocyanin (KLH).

Publications

Species	Application	Title
	ChIP	Front Oncol; The COX10-AS1/miR-641/E2F6 Feedback Loop Is Involved in the Progression of Glioma.; Authors - Liang Liu
	chIP	Cell Death Dis; E2F1 mediated DDX11 transcriptional activation promotes hepatocellular carcinoma progression through PI3K/AKT/mTOR pathway.; Authors - Yan Yu
	WB	Aging Dis; Age-related Loss of miR-124 Causes Cognitive Deficits via Derepressing RyR3 Expression; Authors - Kai Liu
		Immunity; Ribosome-rescuer PELO catalyzes the oligomeric assembly of NOD-like receptor family proteins via activating their ATPase enzymatic activity; Authors - Xiurong Wu
		Nat Commun; Generation of nanobodies from transgenic 'LamaMice' lacking an endogenous immunoglobulin repertoire; Authors - Thomas Eden